The Ki-67 protein interacts with members of the heterochromatin protein I (HPI) family: A potential role in the regulation of higher-order chromatin structure

Journal of Pathology

Volumn 196, Issue 2, 2002, Pages 135-144

  Scholzen, Thomas ^a,b   Endl, Elmar ^a   Wohlenberg, Claudia ^a   Van Sar, Sjaak Der ^a   Cowell, Ian G ^a   Gerdes, Johannes ^a   Singh, Prim B ^a  

^a RESEARCH CENTER BORSTEL   (Germany)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Heterochromatin; HP1; Ki 67; Mitosis; Nucleus

Indexed keywords

BIOCHEMICAL MARKER; GREEN FLUORESCENT PROTEIN; HETEROCHROMATIN PROTEIN 1; KI 67 ANTIGEN; MONOCLONAL ANTIBODY KI 67; NONHISTONE PROTEIN; NUCLEAR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CANCER GROWTH; CANCER RECURRENCE; CANCER SURVIVAL; CARBOXY TERMINAL SEQUENCE; CELL FUNCTION; CELL PROLIFERATION; CHROMATIN STRUCTURE; CONTROLLED STUDY; CORRELATION ANALYSIS; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; PRIORITY JOURNAL; PROGNOSIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; REGULATORY MECHANISM;

ANIMALS; CELL DIVISION; CELL NUCLEUS; CHROMATIN; CHROMOSOMAL PROTEINS, NON-HISTONE; FLUORESCENT ANTIBODY TECHNIQUE; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; INTERPHASE; KI-67 ANTIGEN; LUMINESCENT PROTEINS; PROTEIN ISOFORMS; RECOMBINANT FUSION PROTEINS; TRANSFECTION; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 0036153717     PISSN: 00223417     EISSN: None     Source Type: Journal    
DOI: 10.1002/path.1016     Document Type: Article

References (42)

1. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation
2. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67
3. The Ki-67 protein: From the known and the unknown
4. The cell proliferation-associated antigen of antibody Ki-67: A very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins
5. The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression
6. Zellbiologische Untersuchungen zu intrazellulärem Transport, Funktion und Regulation des proliferations-assoziierten Antigens Ki-67
7. The FHA domain: A putative nuclear signalling domain found in protein kinases and transcription factors
8. Localisation of the Ki-67 antigen within the nucleolus. Evidence for a fibrillarin-deficient region of the dense fibrillar component
9. 1 cells
10. Chmadrin: A novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure
11. The biochemical characterization of the DNA binding activity of pKi67
12. Mammalian chromodomain proteins: Their role in genome organisation and expression
13. KAP-1 corepressor protein interacts and colocalizes with heterochromatic and euchromatic HP1 proteins: A potential role for Kruppel-associated box-zinc finger proteins in heterochromatin-mediated gene silencing
14. Interaction with members of the heterochromatin protein 1 (HP1) family and histone deacetylation are differentially involved in transcriptional silencing by members of the TIF1 family
15. Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins
16. Heterochromatin protein 1 modifies mammalian PEV in a dose- and chromosomal-context-dependent manner
17. M31, a murine homolog of Drosophila HP1, is concentrated in the XY body during spermatogenesis
18. The murine heterochromatin protein M31 is associated with the chromocenter in round spermatids and is a component of mature spermatozoa
19. INCENP centromere and spindle targeting: Identification of essential conserved motifs and involvement of heterochromatin protein HP1
20. Dynamic associations of heterochromatin protein 1 with the nuclear envelope
21. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections
22. A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin
23. M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1), localises to euchromatin within interphase nuclei and is largely excluded from constitutive heterochromatin
24. The putative nuclear receptor mediator TIF1alpha is tightly associated with euchromatin
25. Posttranslational modifications of the Ki-67 protein coincide with two major checkpoints during mitosis
26. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
27. New Ki-67-equivalent murine monoclonal antibodies (MIB 1-3) generated against bacterially expressed parts of the Ki-67 cDNA containing three 62 base pair repetitive elements encoding for the Ki-67 epitope
28. Proliferation-associated Ki-67 protein is a target for autoantibodies in the human autoimmune disease systemic lupus erythematosus
29. Construction of an improved host strain for two hybrid screening
30. Yeast two-hybrid library screening
31. Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells
32. Structure and functions of nucleolin
33. Ki67 - Structure, function, and new antibodies
34. Interaction of SP100 with HP1 proteins: A link between the promyelocytic leukemia-associated nuclear bodies and the chromatin compartment
35. Ki-67 detects a nuclear matrix-associated proliferation-related antigen. I. Intracellular localization during interphase
36. Ki-67, detects a nuclear matrix-associated proliferation-related antigen. II. Localization in mitotic cells and association with chromosomes
37. Biochemical characterization of pKi67 with the identification of a mitotic-specific form associated with hyperphosphorylation and altered DNA binding
38. Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus
39. Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53
40. Identification and partial characterization of a Mr 40,000 nucleolar antigen associated with cell proliferation
41. A nucleolar auto-antigen is part of a major chromosomal surface component
42. The forkhead-associated domain of Ki-67 antigen interacts with the novel kinesin-like protein Hk1p2