The effects of cell density, attachment substratum and dexamethasone on spontaneous apoptosis of rat hepatocytes in primary culture

In Vitro Cellular and Developmental Biology - Animal

Volumn 35, Issue 7, 1999, Pages 417-424

  Qiao, Liang ^a   Farrell, Geofrrey C ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

Cell death; Cell density; Cell matrix; Cultured rat hepatocytes; DNA fragmentation

Indexed keywords

DEXAMETHASONE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL ADHESION; CELL DENSITY; DNA DEGRADATION; LIVER CELL; MALE; NONHUMAN; RAT;

ANIMALIA;

EID: 0032813842     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11626-999-0117-2     Document Type: Article

References (39)

1. Alison, M. R.; Sarraf, C. E. Liver cell death: patterns and mechanisms. Gut 35:577-581; 1994.
2. Berry, M. N.; Edwards, A. M.; Barritt, G. J. Monolayer culture of hepatocytes. Isolated hepatocytes preparation, properties, and applications. In: Burdon, R. H.; Van Knippenberg, P. H., ed. Laboratory techniques in biochemistry and molecular biology. Vol. 21. Amsterdam: Elsevier; 1990:265-354.
3. Bissell, D. M.; Guzelian, P. S. Phenotypic stability of adult rat hepatocytes in primary monolayer culture. Ann. N.Y. Acad. Sci. 349:85-98; 1980.
4. Boudreau, N.; Sympson, C. J.; Werb, Z.; Bissell, M. J. Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix. Science. (Wash DC) 267:891-893; 1995.
5. Bursch, W.; Oberhammer, F.; Schulte-Hermann, R. Cell death by apoptosis and its protective role against disease. Trends Pharmacol Sci. 13:245-251; 1992.
6. Chaya, D.; Fougere-Deschatrelte, C.; Weiss, M. C. Spontaneous apoptosis in a rat hepatoma cell line and its inhibition by dexamethasone. Cell Death Differ. 3:97-104; 1996.
7. Collins, M. K. L; Lopez-Rivas, A. The control of apoptosis in mammalian cells. Trends Biol. Sci. 18:307-309; 1993.
8. Duke, R. C.; Cohen, J. J.; Boehme, S. A.; Lenardo, M. J.; Surh, C. D.; Kishimoto, H.; Sprent, J. Morphological, biological, and flow cytometric assays of apoptosis. In: Coligan, J. E.; Kruisbeek, A. M.; Margulies, D. H.; Shevach, E. M.; Strober, W., ed. Current protocols in immunology. Vol 1. New York: John Wiley & Sons; 1995:3.17.1-3.17.33.
9. Fladmark, K. E.; Gjertsen, B. T.; Doøskeland, S. O.; Vintermyr, O. K. Fas/ APO-1 (CD 95)-induced apoptosis of primary hepatocytes is inhibited by cAMP. Biochem. Biophys. Res. Commun. 232:20-25; 1997.
10. Fukuda, K.; Kojiro, M.; Chiu, J. F. Induction of apoptosis by transforming growth factor-β1 in the rat hepatoma cell line McA-RH7777: a possible association with tissue transglutaminase expression. Hepatology 18:945-953; 1993.
11. Garner, M. J.; Fletcher, K.; Pogson, C. I.; Strain, A. J. The mitogenic response to EGF of rat hepatocytes cultured on laminin-rich gels (EHS) is blocked downstream of receptor tyrosine-phosphorylation. Biochem. Biophys. Res. Commun. 228:238-245; 1996.
12. Goldworthy, T. L.; Fransson-Steen, R.; Maronpot, R. R. Importance of and approaches to quantification of hepatocyte apoptosis. Toxicol. Pathol. 24:24-35; 1996.
13. Gressner, A. M.; Lahme, B.; Mannherz, H. G.; Polzar, B. TGF-β-mediated hepatocellular apoplosis by rat and human hepatoma cells and primary rat hepatocytes. J. Hepatol. 26:1079-1092; 1997.
14. Guguen-Guillouzo, C.; Corlu, A. Recent progress on long-term hepatocyte primary cultures: importance of cell microenvironments. Cytotechnology 11:S3-S5; 1993.
15. Hayashi, N.; Mila, E. Fas system and apoptosis in viral hepatitis. J. Gastroenterol. Hepatol. 12:S223-S226; 1997.
16. Higaki, K.; Yano, H.; Kojiro, M. Fas antigen expression and its relationship with apoptosis in human hepatocellular carcinoma and noncancerous tissues. Am. J. Pathol. 149:429-137; 1996.
17. Higuchi, H.; Kurose, I.; Kato, S.; Miura, S.; Ishii, H. Ethanol-induced apoptosis and oxidative stress in hepatocytes. Alcohol Clin. Exp. Res. 20:340A-346A; 1996.
18. Jones, R. A.; Johnson, V. L.; Buck, N. R.; Dobrota, M.; Hinton, R. H.; Chow, S. C.; Kass, G. E. Fas-mediated apoptosis in mouse hepatocytes involves the processing and activation of caspases. Hepatology 27:1632-1642; 1998.
19. Kleinman, H. K.; McGarvey, M. L.; Hassell, J. L.; Star, V. L.; Cannon, F. B.; Laurie, G. W.; Martin, G. R. Basement membrane complexes with biological activity. Biochemistry. 25:312-318; 1986.
20. Kreamer, B. L.; Staecker, J. L.; Sawada, N.; Sattler, G. L.; Hsia, M. T. S.; Pitot, H. C. Use of low-speed, iso-density Percoll centrifugation method to increase the viability of isolated rat hepatocyte preparations. In Vitro Cell. Dev. Biol. 22:201-211; 1986.
21. Maeda, S.; Kimura, H.; Koya, N.; Lin, K. H.; Saito, T. Cell density-dependent DNA fragmentation and its suppression by heparin in primary culture of rat hepatocytes. Biochem. Biophys. Res. Commun. 195:270-275; 1993.
22. Maeda, S.; Suzuki, A.; Lin, K. H.; Inagaki, H.; Saito, T. DNA fragmentation induced in high-cell-density culture of primary rat hepatocytes is an active process dependent on energy availability, gene expression, and calmodulin. J. Biochem. 118:1161-1165; 1995.
23. Margolis, B.; Rhee, S. G.; Felder, S.; Mervic, M.; Lyall, R.; Levitzki, A.; Ullrich, A.; Zilberstein, A.; Schlessinger, J. EGF induces tyrosine phosphorylation of phospholipase C-II: a potential mechanism for EGF receptor signaling. Cell 57:1101-1107; 1989.
24. McClain, D. E.; Kalinich, J. F; Ramakrishnan, N. Trolox inhibits apoptosis in irradiated MOLT-4 lymphocytes. FASEB J. 9:1345-1354; 1995.
25. McConkey, D. J.; Hartzell, P.; Nicotera, P.; Wyllie, A. H.; Orrenius, S. Stimulation of endogenous endonuclease activity in hepatocytes exposed to oxidative stress. Toxicol. Lett. 42:123-130; 1988.
26. Meredith, J. E.; Fazeli, B.; Schwartz, M. A. The extracellular matrix as a cell survival factor. Mol. Biol. Cell 4:953-961; 1993.
27. Migliorati, G.; Nicoletti, I.; Nocentini, G.; Pagliacci, M. C.; Riccardi, C. Dexamethasone and interleukins modulate apoptosis of murine thymocytes and peripheral T-lymphocytes. Pharmacol. Res. 30:43-52; 1994.
28. Oberhammer, F. A.; Pavelka, M.; Sharma, S.; Tiefebacher, R.; Purchio, A. F.; Bursch, W.; Schulte-Hermann, R. Induction of apoptosis in cultured hepatocytes and in regressing liver by transforming growth factor β1. Proc. Natl. Acad. Sci. 89:5408-5412; 1992.
29. Ohno, K.; Ammann, P.; Fasciati, R.; Maier, P. Transforming growth factor β1 preferentially induces apoptotic cell death in rat hepatocytes cultured under pericentral-equivalenl conditions. Toxicol. Appl. Pharmacol. 132:227-236; 1995.
30. Patel, T.; Gores, G. J. Apoptosis and hepatobiliary disease. Hepatology 21:1725-1741; 1995.
31. Pilot, H. C. Hepatocyte death in hepatocarcinogenesis. Hepatology 28:1-5; 1998.
32. Haff, M. C.; Barres, B. A.; Burne, J. F.; Coles, H. S.; Ishizaki, Y.; Jacobson, M. D. Programmed cell death and control of cell survival: lessons from the nervous system. Science (Wash DC) 262:695-700; 1993.
33. Ramakrishnan, N.; Catravas, G. N. N-(2-mercaptoethyl)-1.3-propanediamine (WR-1605) protects thymocytes from programmed cell death. J. Immunol. 148:1817-1821; 1992.
34. Sanchez, V.; Lucas, M.; Sanz, A.; Goberna, R. Decreased protein kinase C activity is associated with programmed cell death (apoptosis) in freshly isolated rat hepatocytes. Biosci. Rep. 12:199-206; 1992.
35. Searle, J.; Harmon, B. V.; Bishop, C. J.; Kerr, J. F. R. The significance of cell death by apoptosis in hepatobiliary disease. J. Gastrol. Hepatol. 2:77-96; 1987.
36. Seglen, P. O. Preparation of isolated rat liver cells. Methods Cell Biol. 13:29-83; 1976.
37. Williams, G. T.; Smith, C. A.; McCarthy, N. J.; Grimes, E. A. Apoptosis: final control point in cell biology. Trends Cell Biol. 2:263-267; 1992.
38. Yamamoto, M.; Fukuda, K.; Miura, N.; Suzuki, R.; Kido, T.; Komatsu, Y. Inhibition by dexamethasone of transforming growth factor beta1-induced apoptosis in rat hepatoma cells: a possible association with Bcl-xL. Hepatology 27:959-966; 1998.
39. Yokoyama, I.; Hayakawa, A.; Hayashi, S.; Kobayashi, T.; Negita, M.; Katayama, A.; Nagasaka, R.; Namii, Y.; Kojima, T.; Koike, C.; Uchida, K.; Takagi, H. Fas antigen expression and apoptosis induction of in vitro cultured hepatocytes with high concentrations of cyclosporine A. Transplant. Proc. 28:1383-1384; 1996.