Post-isolation inducible nitric oxide synthase gene expression due to collagenase buffer perfusion and characterization of the gene regulation in primary cultured murine hepatocytes

Journal of Biochemistry

Volumn 124, Issue 5, 1998, Pages 892-899

  Wang, Haoran ^a,b   Gao, Xiangrong ^a   Fukumoto, Soji ^a   Tademoto, Sayuri ^a   Sato, Kenzo ^a   Hirai, Kazumitsu ^a  

^a TOTTORI UNIVERSITY   (Japan)

^b HEBEI MEDICAL UNIVERSITY   (China)

Author keywords

Collagenase buffer perfusion; Gene expression; Inducible nitric oxide synthase; Murine primary hepatocyte; Nuclear factor B

Indexed keywords

BUFFER; COLLAGENASE; CYCLOHEXIMIDE; DACTINOMYCIN; EGTAZIC ACID; LIPOPOLYSACCHARIDE; MESSENGER RNA; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; PYRROLIDINE DITHIOCARBAMATE;

ANIMAL CELL; ARTICLE; CELL CULTURE; DRUG EFFECT; GENE CONTROL; GENE EXPRESSION; GENETIC TRANSCRIPTION; LIVER CELL; MALE; MOUSE; NONHUMAN; NORTHERN BLOTTING; PROTEIN SYNTHESIS;

ANIMALIA; MURINAE;

EID: 0031752907     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022204     Document Type: Article

References (41)

1. Moncada, S. and Higgs, A. (1993) The L-arginine-nitric oxide pathway. N. Engl. J. Med. 329, 2002-2012
2. Nathan, C. and Xie, Q-W. (1994) Nitric oxide synthases: roles, tolls, and controls. Cell 78, 915-918
3. Griffith, O.W. and Stuehr, D.J. (1995) Nitric oxide synthase: properties and catalytic mechanism. Annu. Rev. Physiol. 57, 707-736
4. Crane, B.R., Arvai, A.S., Gachhui, R., Wu, C., Ghosh, D.K., Getzoff, E.D., Stuehr, D.J., and Tainer, J.A. (1997) The structure of nitric oxide synthase oxygenase domain and inhibitor complexes. Science 278, 425-431
5. Schmidt, H.H.H.W. and Walter, U. (1994) NO at work. Cell 78, 919-925
6. Beckman, J.S., Beckman, T.W., Chen, J., Marshall, P.A., and Freeman, B.A. (1990) Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and Superoxide. Proc. Natl. Acad. Sci. USA 87, 1620-1624
7. Moro, M.A., Darley-Usmar, V.M., Goodwin, D.A., Read, N.G., Zamora-Pino, R., Feelisch, M., Radomski, M.W., and Moncada, S. (1994) Paradoxial fate and biological action of peroxynitrite on human platelets. Proc. Natl. Acad. Sci. USA 91, 6702-6706
8. Beckman, J.S. (1991) The double-edged role of nitric oxide in brain function and superoxide-mediated injury. J. Dev. Physiol. 15, 53-59
9. Stuehr, D.J. and Marletta, M.A. (1985) Mammalian nitrate biosynthesis: mouse macrophages produce nitrite and nitrate in response to Escherichia coli lipopolysaccharide. Proc. Natl. Acad. Sci. USA 82, 7738-7742
10. Palmer, R.M.J., Ashton, D.S., and Moncada, S. (1988) Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature 333, 664-666
11. Geller, D.A., Nussler, A.K., Silvio, M.D., Lowenstein, C.J., Shapiro, R.A., Wang, S.C., Simmons, R.L., and Billiar, T.R. (1993) Cytokines, endotoxin, and glucocorticoids regulate the expression of inducible nitric oxide synthase in hepatocytes. Proc. Natl. Acad. Sci. USA 90, 522-526
12. Tachado, S.D., Gerold, P., McConville, M.J., Baldwin, T., Quilinci, D., Schwarz, R.T., and Schofield, L. (1996) Glycosylphosphatidylinositol toxin of Plasmodium induces nitric oxide synthase expression in macrophages and vascular endothelial cells by a protein tyrosine kinase-dependent signaling pathway. J. Immunol. 156, 1897-1907
13. Wang, H., Tanihata, T., Fukumoto, S., and Hirai, K. (1997) Excretory/secretory products of plerocercoids of Spirometra erinaceieuropaei induce the expression of inducible nitric oxide synthase mRNA in murine hepatocytes. Int. J. Parasitol. 27, 367-375
14. Saad, B., Frei, K., Scholl, F.A., Fontana, A., and Maier, P. (1995) Hepatocyte-derived interleukin-6 and tumor-necrosis factor a mediate the lipopolysaccharide-induced actute-phase response and nitric oxide release by cultured rat hepatocytes. Eur. J. Biochem. 229, 349-355
15. Lee, B-S., Rang, H-S., Pyun, K-H., and Choi, I. (1997) Roles of tyrosine kinases in the regulation of nitric oxide synthesis in murine liver cells: modulation of NF-(Greek small letter script kappa)B activity by tyrosine kinases. Hepatology 25, 913-919
16. Kurose, I., Kato, S., Ishii, H., Fukumura, D., Miura, S., Suematsu, M., and Tsuchiya, M. (1993) Nitric oxide mediates lipopolysaccharide-induced alteration of mitochondrial function in cultured hepatocytes and isolated perfused liver. Hepatology 18, 380-388
17. Uematsu, M., Ohara, Y., Navas, J.P., Nishida, K., Murphy, Y.J., Alexander, R.W., Nerem, R.M., and Harrison, D.G. (1995) Regulation of endothelial cell nitric oxide synthase mRNA expression by shear stress. Am. J. Physiol. 269, C1371-C1378
18. Takahashi, M., Ishida, T., Traub, O., Corson, M.A., and Berk, B.C. (1997) Mechanotransduction in endothelial cells: temporal signaling events in response to shear stress. J. Vasc. Res. 34, 212-219
19. Milligan, S.A., Owens, M.W., and Grisham, M.B. (1996) Inhibition of IkappaB-alpha and IkappaB-beta proteolysis by calpain inhibitor I blocks nitric oxide synthesis. Arch. Biochem. Biophys. 335, 388-395
20. Hill, G.E., Taylor, J.A., and Robbins, R.A. (1997) Differing effect of aprotinin and epsilon-aminocaproic acid on cytokine-induced inducible nitric oxide synthase expression. Ann. Thorac. Surg. 63, 74-77
21. Seglen, P.O. (1976) Preparation of isolated liver cells. Methods Cell. Biol. 13, 29-83
22. Klauning, J.E., Goldblatt, P.J., Hinton, D.E., Lipsky, M.M., Chacko, J., and Trump, B.F. (1981) Mouse liver cell culture. 1. Hepatocyte isolation. In Vitro 17, 913-925
23. Deng, W., Thiel, B., Tannenbaum, C.S., Hamilton, T.A., and Stuehr, D. (1993) Synergistic cooperation between T cell lymphokines for induction of the nitric oxide synthase gene in murine peritoneal macrophages. J. Immunol. 151, 322-329
24. Financsek, I., Mizumoto, K., and Muramatsu, M. (1982) Nucleotide sequence of the transcription initiation region of a rat ribosomal RNA gene. Gene 18, 115-122
25. Maizels, R.M., Blaxter, M.L., Robertson, B.D., and Selkirk, M.E. (1991) Parasite Antigens, Parasite Genes. A Laboratory Manual for Molecular Parasitology (Maizels, R.M., Blaxter, M.L., Robertson, B.D., and Selkirk, M.E., eds.) pp. 131-143, Cambridge University Press, New York
26. Cave, A.C. and Apstein, C.S. (1996) Polymyxin B, a protein kinase C inhibitor, abolishes preconditioning-induced protection against contractile dysfunction in the isolated blood perfused rat heart. J. Mol. Cell. Cardiol. 28, 977-987
27. Pugazhenthi, S., Mantha, S.V., and Khandelwal, R.L. (1990) Inhibitory effect of H-7, H-8 and polymyxin B on liver protein kinase C-induced phosphorylation of endogenous substrates. Biochem. Int. 20, 941-948
28. Xie, Q-W., Whisnant, R., and Nathan, C. (1993) Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by Interferon-γ and bacterial lipopolysaccharide. J. Exp. Med. 177, 1779-1784
29. Xie, Q-W., Kashiwabara, Y., and Nathan, C. (1994) Roles of transcriptional factor NF-(Greek small letter script kappa)B/c-rel in induction of nitric oxide synthesis. J. Biol. Chem. 269, 4705-4708
30. Helyar, L., Bundschuh, D.S., Iaskin, J.L., and Iaskin, D.L. (1994) Induction of hepatic Ito cell nitric oxide production after acute endotoxemia. Hepatology 20, 1509-1515
31. Spiter, J.A. (1994) Cytokine stimulation of nitric oxide formation and differential regulation in hepatocytes and nonparenchymal cells of endotoxemic rats. Hepatology 19, 217-218
32. Mittal, M.K., Gupta, T.K., Lee, F.Y., Sieber, C.C., and Groszmann, R.J. (1994) Nitric oxide modulates hepatic vascular tone in normal rat liver. Am. J. Physiol. 267, G416-G422
33. Curran, R.D., Ferrari, F.K., Kispert, P.H., Stadler, J., Stuehr, D., Simmons, R.L., and Billiar, T.R. (1991) Nitric oxide and nitric oxide-generating compounds inhibit hepatocyte protein synthesis. FASEB. J. 5, 2085-2092
34. Horton, R.A., Ceppi, E.D., Knowles, R.G., and Titheradge, M.A. (1994) Inhibition of hepatic gluconeogenesis by nitric oxide: a comparison with endotoxic shock. Biochem. J. 299, 735-739
35. Stadler, J., Billiar, T.R., Curran, R.D., Stuehr, D.J., Ochao, J.B., and Simmons, R.L. (1991) Effect of exogenous and endogenous nitric oxide on mitochondrial respiration of hepatocytes. Am. J. Physiol. 260, C910-C916
36. Stadler, J., Trockfeld, J., Schmalix, W.A., Brill, T., Seiwert, J.R., Greim, H., and Doehmer, J. (1994) Inhibition of cytochromes P450A by nitric oxide. Proc. Natl. Acad. Sci. USA 91, 3559-3563
37. Weisz, A., Oguchi, S., Cicatiello, L., and Esumi, H. (1994) Dual mechanism for the control of inducible-type NO synthase gene expression in macrophages during activation by Interferon-γ and bacterial lipopolysaccharide. J. Biol. Chem. 269, 8324-8333
38. Paul, A., Pendreign, R.H., and Plevin, R. (1995) Protein kinase C and tryosine kinase pathways regulate lipopolysaccharide-induced nitric oxide synthase activity in RAW264.7 murine macrophages. Br. J. Pharmacol. 114, 482-488
39. Baeuerle, P.A. (1991) The inducible transcription factor NF-(Greek small letter script kappa)B: regulation by distinct protein subunits. Biochim. Biophys. Acta. 1072, 63-68
40. Ziegler-Heitbrock, H.W., Sternsdorf, T., Liese, J., Belohradsky, B., Weber, C., Wedel, A., Schreck, R., Baeuerle, P., and Strobel, M. (1993) Pyrrolidine inhibits NF-(Greek small letter script kappa)B mobilization and TNF production in human monocytes. J. Immunol. 151, 6986-6993
41. Schreck, R., Meier, B., Mannel, D.N., Droge, W., and Baeuerle, P. (1992) Dithiocarbamates as potent inhibitors of nuclear factor-(Greek small letter script kappa)B activation in intact cells. J. Exp. Med. 175, 1181-1194