Gel diffusion assays for endo-β-mannanase and pectin methylesterase can underestimate enzyme activity due to proteolytic degradation: A remedy

Analytical Biochemistry

Volumn 300, Issue 1, 2002, Pages 87-93

  Bourgault, Richard ^a   Bewley, J Derek ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Endo mannanase; Gel diffusion assays; Pectin methylesterase; Proteolytic protection

Indexed keywords

DIFFUSION; FRUITS; GELS;

GEL-DIFFUSION; MANNANASE; OPTIMAL CONCENTRATION; PECTIN METHYLESTERASE; PROTEASE INHIBITOR; PROTEIN CONTENTS; PROTEOLYTIC DEGRADATION; PROTEOLYTIC PROTECTION;

ENZYME ACTIVITY;

BETA MANNOSIDASE; PECTINESTERASE;

ARTICLE; CELL WALL; DIAGNOSTIC ACCURACY; ENZYME ACTIVITY; ENZYME ASSAY; ESTERIFICATION; FRUIT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SERODIAGNOSIS; TOMATO;

CUCUMIS MELO; LYCOPERSICON ESCULENTUM; PRUNUS PERSICA; PRUNUS PERSICA NUCIPERSICA;

EID: 0036147574     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5450     Document Type: Article

References (14)

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10. A single-seed assay for endo-β-mannanase activity from tomato endosperm and radicle tissues
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12. Endo-βmannanase is present in an inactive form in ripening tomato fruits of the cultivar Walter
13. A gel diffusion assay for quantification of pectin methylesterase activity
14. A buffer solution for colorimetric comparison