Enantioselective synthesis of aliphatic (S)-cyanohydrins in organic solvents using hydroxynitrile lyase from Manihot esculenta

Annals of the New York Academy of Sciences

Volumn 799, Issue , 1996, Pages 771-776

  Wajant, Harald ^a   Förster, Siegfried ^a   Sprauer, Achim ^a   Effenberger, Franz ^a   Pfizenmaier, Klaus ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

LYASE; NITRILE; ORGANIC SOLVENT; RECOMBINANT ENZYME;

CASSAVA; CHEMICAL REACTION; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ASSAY; ENZYME PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL FILTRATION; GENE INSERTION; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS;

EID: 0030474957     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1996.tb33290.x     Document Type: Conference Paper

References (7)

1. EFFENBERGER, F., B. HÖRSCH, S. FÖRSTER & T. ZIEGLER. 1990. Enzyme-catalyzed synthesis of (S)-cyanohydrins and subsequent hydrolysis to (S)-α-hydroxy-carboxylic acids. Tetrahedron Lett. 31: 1249-1252.
2. SMITSKAMPS-WILMS, E., J. BRUSSEE, G. J. M. VAN SCHARRENBURG, J. B. SLOOTHAAK & A. VAN DER GEN. 1991. Hydroxynitrile lyases from almond and sorghum as biocatalysts. Reel. Trav. Chim. Pays-Bas 110: 209-215.
3. HUGHES, J., F. J. P. DE C. CARVALHO & M. A. HUGHES. 1994. Purification, characterization, and cloning of α-hydroxynitrile lyase from cassava (Manihot esculenta Crantz). Arch. Biochem. Biophys. 311: 496-502.
4. LAEMMLI, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
5. BLUM, H., H. BEIER & H. J. GROSS. 1987. Improved silver staining of plant proteins, RNA, and DNA in polyacrylamide gels. Electrophoresis 8: 93-99.
6. SELMAR, D., F. J. P. DE C. CARVALHO & E. E. CONN. 1987. A colorimetric assay for α-hydroxynitrile lyase. Anal. Biochem. 166: 208-211.
7. WAJANT, H., S. FÖRSTER, H. BÖTTINGER, F. EFFENBERGER & K. PFIZENMAIER. 1995. Acetone cyanohydrin lyase from Manihot esculenta (cassava) is serologically distinct from other hydroxynitrile lyases. Plant Sci. 108: 1-11.