N-terminal contributions of the γ-subunit of fetal hemoglobin to its tetramer strength: Remote effects at subunit contacts

Protein Science

Volumn 11, Issue 1, 2002, Pages 27-35

  Yagami, Takeshi ^a   Ballard, Barry T ^a   Padovan, Julio Cesar ^b   Chait, Brian T ^b   Popowicz, Anthony M ^b   Manning, James M ^a  

^a NORTHEASTERN UNIVERSITY   (United States)

^b ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Hemoglobin; Long range interaction; Site directed mutagenesis

Indexed keywords

AMINO ACID; ASPARTIC ACID; DIMER; GLUTAMINE; GLYCINE; HEMOGLOBIN A; HEMOGLOBIN F; LEUCINE; OXYGEN; PHENYLALANINE; PROLINE; PROTEIN SUBUNIT; PROTEINASE; TETRAMER; VALINE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; DISSOCIATION CONSTANT; MOLECULAR INTERACTION; OXYGEN AFFINITY; PKA; PLASMODIUM; PRIORITY JOURNAL; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ASPARTIC ACID; CIRCULAR DICHROISM; DIMERIZATION; DOSE-RESPONSE RELATIONSHIP, DRUG; FETAL HEMOGLOBIN; GLUTAMIC ACID; HUMANS; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; ISOLEUCINE; KINETICS; LEUCINE; MASS SPECTROMETRY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; OXYGEN; PHENYLALANINE; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; VALINE;

EID: 0036135327     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.ps.30602     Document Type: Article

References (18)

1. Matrix-assisted laser desorption ionization mass-spectrometry of proteins
2. Transposing sequences between fetal and adult hemoglobins indicates which subunits and regulatory molecule interfaces are functionally related
3. Exchange of subunit interfaces between recombinant adult and fetal hemoglobins. Evidence for a functional inter-relationship among regions of the tetramer
4. The N-terminal sequence affects distant helix interactions in hemoglobin. Implications for mutant proteins from studies on recombinant hemoglobin Felix
5. The crystal structure of human deoxy- haemoglobin at 1.74 A resolution
6. Structure of human fetal deoxyhaemoglobin
7. Mutational analysis of sickle haemoglobin (Hb) gelation
8. 2-terminal residues
9. The acetylation state of human fetal hemoglobin modulates the strength of its subunit interactions: Long-range effects and implications for histone interactions in the nucleosome
10. Subunit dissociations in natural and recombinant hemoglobins
11. Determining subunit dissociation constants in natural and recombinant proteins
12. Properties of a recombinant human hemoglobin double mutant: Sickle hemoglobin with Leu-88(β) at the primary aggregation site substituted by Ala
13. Recombinant human sickle hemoglobin expressed in yeast
14. Purification and characterization of recombinant human sickle hemoglobin expressed in yeast
15. Fetal haemoglobin and malaria
16. Mechanisms of cooperativity and allosteric regulation in proteins
17. Transgenic mice expressing human fetal globin are protected from malaria by a novel mechanism
18. Probing the importance of the amino-terminal sequence of the β- and γ-chains to the properties of normal adult and fetal hemoglobins