Enhancing the anticoagulant potency of soluble tissue factor mutants by increasing their affinity to factor VIIa

Thrombosis and Haemostasis

Volumn 87, Issue 3, 2002, Pages 450-458

  Yang, Jihong ^a   Lee, Geoffrey F ^a   Riederer, Markus A ^a   Kelley, Robert F ^a  

^a GENENTECH INC   (United States)

Author keywords

Anticoagulant; Coagulation factor VIIa; Phage display; Tissue factor

Indexed keywords

AMINO ACID; ANTICOAGULANT AGENT; BLOOD CLOTTING FACTOR 10A; BLOOD CLOTTING FACTOR 7A; LYSINE; THROMBOPLASTIN; THROMBOPLASTIN ALANINE; THROMBOPLASTIN ALANINE 2; THROMBOPLASTIN ALANINE 3; THROMBOPLASTIN ALANINE 4; UNCLASSIFIED DRUG; FIBRINOLYTIC AGENT;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTICOAGULATION; ARTERY THROMBOSIS; ARTICLE; BINDING AFFINITY; BLOOD CLOTTING; CONTROLLED STUDY; DRUG INHIBITION; DRUG POTENCY; GENE MUTATION; MUTANT; NONHUMAN; PHAGE DISPLAY; PHAGEMID; PRIORITY JOURNAL; PROTEIN DEGRADATION; RABBIT; SOLUBILITY; SURFACE PLASMON RESONANCE; DRUG ANTAGONISM; GENETICS; HUMAN; METABOLISM; MUTATION; PROTEIN BINDING; PROTEIN ENGINEERING;

ANTICOAGULANTS; FACTOR VIIA; FIBRINOLYTIC AGENTS; HUMANS; MUTATION; PROTEIN BINDING; PROTEIN ENGINEERING; SOLUBILITY; SURFACE PLASMON RESONANCE; THROMBOPLASTIN;

EID: 0036123295     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-0037-1613025     Document Type: Article

References (43)

1. The coagulation cascade: Initiation, maintenance, and regulation
2. Structural biology of tissue factor, the initiator of thrombogenesis in vivo
3. Tissue factor and hemostasis
4. Factor VII binding to tissue factor in reconstituted phospholipid vesicles: Induction of cooperativity by phosphatidylserine
5. 2+, phospholipids and tissue-factor apoprotein to the activation of human blood-coagulation factor X by activated factor VII
6. Biochemistry and physiology of blood coagulation
7. The tissue factor pathway: How it has become a "Prima Ballerina"
8. Selective cellular expression of tissue factor in human tissues
9. Localization of tissue factor in the normal vessel wall and in the atherosclerotic plaque
10. Tissue factor accelerates the activation of coagulation factor VII: The role of a bifunctional coagulation cofactor
11. Activation of factor VII bound to tissue factor: A key early step in the tissue factor pathway of blood coagulation
12. Binding of human factors VII and VIIa to a human bladder carcinoma cell line (J82)
13. Activation of factor IX by the reaction product of tissue factor and factor VII: Additional pathway for initiating blood coagulation
14. Factor IX is activated in vivo by the tissue factor mechanism
15. A comparison of human prothrombin, factor IX (Christmas factor), factor X (Stuart factor), and protein S
16. The crystal structure of the complex of blood coagulation factor VIIa with soluble tissue factor
17. Proteolytic activation of human factors IX and X by recombinant human factor VIIa: Effects of calcium, phospholipids, and tissue factor
18. Regulation of tissue factor gene expression in the monocyte procoagulant response to endotoxin
19. Macrophages, smooth muscle cells, and tissue factor in unstable angina
20. Differential expression of tissue factor protein in directional atherectomy specimens from patients with stable and unstable coronary syndromes
21. Identification of active tissue factor in human coronary atheroma
22. Local inhibition of tissue factor reduces the thrombogenicity of disrupted human atherosclerotic plaques
23. The tissue factor pathway of coagulation: Factor VII, tissue factor, and tissue factor pathway inhibitor
24. Molecular and cellular mechanisms of atherogenesis: Studies of human lesions linked with animal modeling
25. Peptide exosite inhibitors of factor VIIa as anticoagulants
26. Thrombosis: New antithrombotic agents
27. The new anticoagulants: New opportunities, new issues
28. Epitope location on tissue factor determines the anticoagulant potency of monoclonal anti-tissue factor antibodies
29. A soluble tissue factor mutant is a selective anticoagulant and antithrombotic agent
30. Inhibition of arterial thrombosis by a soluble tissue factor mutant and active site-blocked factors IXa and Xa in the guinea pig
31. Analysis of the factor VIIa binding site on human tissue factor: Effects of tissue factor mutations on the kinetics and thermodynamics of binding
32. Monovalent phage display: A method for selecting variant proteins from random libraries
33. Phage display of peptide libraries on protein scaffolds
34. A novel soluble tissue factor variant with an altered factor VIIa binding interface
35. DNA sequencing with chain-terminating inhibitors
36. Rapid and efficient site-specific mutagenesis without phenotypic selection
37. Endothelial cells stimulated with tumor necrosis factor-a express varying amounts of tissue factor resulting in inhomogenous fibrin deposition in a native blood flow system
38. Active site-blocked factors VIIa and IXa differentially inhibit fibrin formation in a human ex vivo thrombosis model
39. Immunologic quantification of fibrin deposition in thrombi formed in flowing native human blood
40. Additivity of mutational effects in proteins
41. The tissue factor region that interacts with substrates factor IX and factor X
42. The tissue factor region than interacts with Factor Xa in the activation of Factor VII
43. Structural basis for the anticoagulant activity of the thrombin-thrombomodulin complex