Semireannealing, single-stranded conformational polymorphism: A novel and effective tool for the diagnosis of T-cell clonality

Diagnostic Molecular Pathology

Volumn 11, Issue 2, 2002, Pages 67-74

  Crisi, Giovanna Maria ^a   Emanuel, Janet Rettig ^a   Johnson, Chaline ^a   Crotty, Paul ^a   Costa, Jose' ^a   Tallini, Giovanni ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Gene rearrangement; Polymerase chain reaction; Semi reannealing single stranded conformational polymorphism; Single stranded conformational polymorphism; T cell lymphoma; T cell receptor gene

Indexed keywords

SINGLE STRANDED DNA; T LYMPHOCYTE RECEPTOR GAMMA CHAIN;

ARTICLE; B CELL LYMPHOMA; CLONAL VARIATION; DNA DENATURATION; DNA FINGERPRINTING; GENE REARRANGEMENT; HUMAN; HUMAN TISSUE; LYMPHOPROLIFERATIVE DISEASE; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SINGLE STRAND CONFORMATION POLYMORPHISM; T CELL LYMPHOMA; T LYMPHOCYTE;

BASE SEQUENCE; CLONE CELLS; DNA, NEOPLASM; ELECTROPHORESIS, CAPILLARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE REARRANGEMENT; HUMANS; LYMPH NODES; LYMPHOMA, B-CELL; LYMPHOMA, T-CELL; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; PSEUDOLYMPHOMA; RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

EID: 0036102977     PISSN: 10529551     EISSN: None     Source Type: Journal    
DOI: 10.1097/00019606-200206000-00002     Document Type: Article

References (16)

1. Immunophenotypic and antigen receptor gene rearrangement analysis in T cell neoplasia
2. A simplified method of detection of clonal rearrangements of the T-cell receptor-γ chain gene
3. The clinical significance of antigen receptor gene rearrangements in lymphoid neoplasia
4. Improved polymerase chain reaction detection of clonal T-cell lymphoid neoplasms
5. The rearrangement of immunoglobin and T cell receptor genes in human lymphoproliferative disorders
6. An automated semiquantitative B and T cell clonality assay
7. PCR amplification from paraffin embedded tissues. Effects of fixative and fixation time
8. Detection of T-cell receptor gamma gene rearrangement in lymphoproliferative disorders by temperature gradient gel electrophoresis
9. Rapid, nonradioactive detection of clonal T-cell receptor gene rearrangements in lymphoid neoplasms
10. Detection of clonal rearrangement of the T-cell receptor gamma gene by polymerase chain reaction and single-strand conformation polymorphism (PCRSSCP)
11. Single, reliable detection of T cell clones by PCR-LIS-SSCP analysis of TCR-gamma rearrangement
12. Detection of clonal T-cell receptor gene rearrangements in paraffin-embedded tissue by polymerase chain reaction and nonradioactive single strand conformational polymorphism analysis
13. A rapid polymerase chain reaction-based technique for detecting clonal T-cell receptor gene rearrangements in cutaneous T-cell lymphomas of both varieties
14. Detection of clonal T-cell receptor gene rearrangement by PCR/Temporal Temperature Gradient Gel Electrophoresis
15. Highly sensitive nonradioactive single-strand conformational polymorphism. Detection of Ki-ras mutations