Detection of clonal T-cell receptor-gamma gene rearrangement by PCR/temporal temperature gradient gel electrophoresis

American Journal of Clinical Pathology

Volumn 116, Issue 4, 2001, Pages 527-534

  Zhu, Dan ^a   Kadin, Marshall E ^b   Samoszuk, Michael ^a  

^a QUEST DIAGNOSTICS NICHOLS INSTITUTE   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

PCR; Polymerase chain reaction; T cell lymphoma; Temporal temperature gradient gel electrophoresis; TTGE

Indexed keywords

FORMALDEHYDE; PARAFFIN; T LYMPHOCYTE RECEPTOR GAMMA CHAIN;

ARTICLE; BASE MISPAIRING; BLOOD SAMPLING; CLINICAL ARTICLE; CORRELATION ANALYSIS; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DNA DENATURATION; DNA DETERMINATION; GEL ELECTROPHORESIS; GENE AMPLIFICATION; GENE REARRANGEMENT; HUMAN; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; T LYMPHOCYTE RECEPTOR GENE; TEMPERATURE MEASUREMENT; TEMPERATURE SENSITIVE CLONE; TEMPORAL TEMPERATURE GRADIENT GEL ELECTROPHORESIS; TISSUE FIXATION;

BASE COMPOSITION; BASE PAIRING; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE REARRANGEMENT, GAMMA-CHAIN T-CELL ANTIGEN RECEPTOR; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TEMPERATURE;

EID: 0035735617     PISSN: 00029173     EISSN: None     Source Type: Journal    
DOI: 10.1309/8KMV-T6BM-G9PC-EK24     Document Type: Article

References (17)

1. Flug F, Pelicci PG, Bonett F, et al. T-cell receptor gene rearrangements as markers of lineage and clonality in T-cell neoplasms. Proc Natl Acad Sci U S A. 1985;82:3460-3464.
2. Yanagi Y, Yoshikai Y, Leggett K, et al. A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. Nature. 1984;308:145-149.
3. Waldmann TA, Davis MM, Bongiovanni K, et al. Rearrangements of genes for the antigen receptor on T cells as markers of lineage and clonality in human lymphoid neoplasms. N Engl J Med. 1985;313:776-783.
4. Raulet DH. The structure, function, and molecular genetics of the gamma/delta T cell receptor. Annu Rev Immunol. 1989;7:175-207.
5. Theodorou I, Delfau MH, Bigorgne C, et al. Cutaneous T-cell infiltrates: analysis of T-cell receptor gamma gene rearrangement by polymerase chain reaction and denaturing gradient gel electrophoresis. Blood. 1995;86:305-310.
6. Spagnolo DV, Taylor J, Carrello S, et al. Southern blot analysis of lymphoproliferative disorders: use and limitations in routine surgical pathology. Pathology. 1994;26:268-275.
7. Murphy M, Signoretti S, Kadin ME, et al. Detection of TCR-gamma gene rearrangements in early mycosis fungoides by non-radioactive PCR-SSCP. J Cutan Pathol. 2000;27:228-234.
8. Anderson WK, Li N, Bhawan J. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR/DGGE)-based detection of clonal T-cell receptor gamma gene rearrangements in paraffin-embedded cutaneous biopsies in cutaneous T-cell lymphoproliferative diseases. J Cutan Pathol. 1999;26:176-182.
9. Wood GS, Tung RM, Haeffner AC, et al. Detection of clonal T-cell receptor gamma gene rearrangements in early mycosis fungoides/Sézary syndrome by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE). J Invest Dermatol. 1994;103:34-41.
10. Menke MAOH, Tiemann M, Vogelsang D, et al. Temperature gradient gel electrophoresis for analysis of a polymerase chain reaction-based diagnostic clonality assay in the early stage of cutaneous T-cell lymphomas. Electrophoresis. 1995;16:733-738.
11. Bourguin A, Tung R, Galili N, et al. Rapid, nonradioactive detection of clonal T-cell receptor gene rearrangements in lymphoid neoplasms. Proc Natl Acad Sci U S A. 1990;87:8536-8540.
12. Orita M, Suzuki Y, Sekiya T, et al. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics. 1989;5:874-879.
13. Yoshino K, Nishigaki K, Husimi Y. Temperature sweep gel electrophoresis: a simple method to detect point mutations. Nuckic Acids Res. 1991;19:3153.
14. Chen TJ, Boles RG, Wong LJ. Detection of mitochondrial DNA mutations by temporal temperature gradient gel electrophoresis. Clin Chem. 1999;45:1162-1167.
15. Alkan S, Cosar E, Ergin M, et al. Detection of T-cell receptor-gamma gene rearrangement in lymphoproliferative disorders by temperature gradient gel electrophoresis. Arch Pathol Lab Med. 2001;125:202-207.
16. Dereure O, Levi E, Kadin ME. T-cell clonality in pityriasis lichenoides et varioliformis acuta: a heteroduplex analysis of 20 cases. Arch Dermatol. 2000;136:1483-1486.
17. Posnett DN, Sinha R, Kabak S, et al. Clonal populations of T cells in normal elderly humans: the T cell equivalent to ́benign monoclonal gammapathy.́ J Exp Med. 1994;179:609-618.