Highly sensitive nonradioactive single-strand conformational polymorphism: Detection of Ki-ras mutations

Diagnostic Molecular Pathology

Volumn 5, Issue 4, 1996, Pages 260-264

  Emanuel, Janet Rettig ^a   Damico, Christine ^a   Ahn, Susan ^a   Bautista, Dolores ^a   Costa, Jose ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Ki ras; Nonradioactive; SSCP; SYBR Green II

Indexed keywords

SINGLE STRANDED DNA;

ARTICLE; GENE FREQUENCY; GENE MUTATION; HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM;

DNA; DNA MUTATIONAL ANALYSIS; ELECTROPHORESIS; FLUORESCENT DYES; GENES, RAS; HUMANS; ORGANIC CHEMICALS; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 0029955066     PISSN: 10529551     EISSN: None     Source Type: Journal    
DOI: 10.1097/00019606-199612000-00006     Document Type: Article

References (10)

1. Ainsworth PJ, Surh LC, Coulter-Mackie MB. Diagnostic single strand conformational polymorphism (SSCP): a simplified non-radioisotopic method as applied to Tay-Sachs B1 variant. Nuclei Acids Res 1991;20:1005-9.
2. Chaubert P, Bautista D, Benhattar J. An improved method for rapid screening of DNA mutations by non-radioactive single-strand conformation polymorphism procedure. Biotechniques 1993;15:586.
3. Makino R, Yazyu H, Kishimoto Y, Sekiya T, K. Hayashi. F-SSCP: fluorescence-based polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis. PCR Methods Appl 1992;2:10-3.
4. Mohabeer AJ, Hiti AL, Martin WJ. Non-radioactive single-strand conformation polymorphism (SSCP) using the Pharmacia "PhastSystem." Nucleic Acids Res 1991;19:3154.
5. Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci USA 1989;86:2766-70.
6. Orita M, Suzuki Y, Sekiya T, Hayashi K. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 1989;5: 874-9.
7. Peng H, Du MIJ, Isaacson PG, Pan L. High-resolution SSCP Analysis using polyacrylamide-agarose composite gel and background-free silver-staining method. Biotechniques 1995;19:410-4.
8. Soto D, Sukumar S. Improved detection of mutations in the p53 gene in human tumors as single-stranded conformation polymorphisms and double-stranded heteroduplex DNA. PCR Methods Applic 1992;2:96-8.
9. Spinardi L, Mazars R, Theillet C. Protocols for an improved detection of point mutations by SSCP. Nucleic Acids Res 1991;19:4009.
10. Yap EPH, McGee JOD. Non-isotopic SSCP and competitive PCR for DNA quantification: p53 in breast cancer. Nucleic Acids Res 1992;20:145.