PKC-ζ is required in EGF protection of microtubules and intestinal barrier integrity against oxidant injury

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 282, Issue 5 45-5, 2002, Pages

  Banan, A ^a,c   Fields, J Z ^a   Talmage, D A ^b   Zhang, L ^a   Keshavarzian, A ^a  

^a RUSH UNIVERSITY   (United States)

^b Och Spine at New York Presbyterian Hospitals   (United States)

^c RUSH UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Caco 2 cells; Cytoskeleton; Epidermal growth factor; Growth factors; Gut barrier; Inflammatory bowel disease; Protection; Protein kinase C isoforms; Transfection

Indexed keywords

EPIDERMAL GROWTH FACTOR; ISOENZYME; PROTEIN KINASE C ZETA; TUBULIN;

ARTICLE; CELL STRAIN CACO 2; CELL STRAIN HT29; CONTROLLED STUDY; CYTOSKELETON; ENZYME ACTIVATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MICROTUBULE; MONOLAYER CULTURE; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0036086444     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.00284.2001     Document Type: Article

References (60)

1. Protein kinase C-α modulates growth and differentiation in Caco-2 cells
2. Protein kinase C modulator effects on parathyroid hormone-induced intracellular calcium and morphologic changes in UMR 106-H 5 osteoblastic cells
3. Assembly of the tight junction. The role of diacylglycerol
4. Phosphatidylcholine could be the source of 1,2-diacylglycerol which activates protein kinase C in EGF stimulated colon carcinoma cells (HT-29)
5. Ethanol-induced barrier dysfunction and its prevention by growth factors in human intestinal monolayers: Evidence for oxidative and cytoskeletal mechanisms
6. Role of the microtubule cytoskeleton in protection by epidermal growth factor and transforming growth factor-α against oxidant-induced barrier disruption in a human colonic cell line
7. iNOS upregulation mediates oxidant-induced disruption of F-actin and the permeability barrier of intestinal monolayers
8. Key role of general protein kinase C activation in EGF-induced protection of the microtubule cytoskeleton and intestinal epithelial barrier against oxidant injury
9. Nitric oxide and its metabolites mediate ethanol-induced microtubule disruption and intestinal barrier dysfunction
10. Targeted molecular inhibition of phospholipase C-γ prevents EGF mediated protection of the microtubule cytoskeleton and intestinal epithelial barrier function against oxidant injury
11. The β1-isoform of protein kinase C mediates epidermal growth factor protection of the microtubules and barrier integrity of intestinal monolayers against oxidant damage
12. Polyamines are required for microtubule formation during mucosal ulcer healing
13. Protection against ethanol injury by prostaglandins in a human intestinal cell line: Role of microtubules
14. Relationship between polyamines, cytoskeletal distribution, and gastric mucosal ulcer healing in rats
15. Carbonylation and disassembly of the F-actin in oxidant-induced barrier dysfunction and its prevention by epidermal growth factor and transforming growth factor-α in a human intestinal cell line
16. G protein kinase C-α activity inversely modulates invasion and growth of intestinal cells
17. Role of protein kinase C in modulating epidermal growth factor- and phorbol ester-induced mammary epithelial cell growth in vitro
18. Expression of four protein kinase C isoforms in rat fibroblasts
19. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye binding
20. The role of protein kinase C isozymes in TNF-α-induced cytotoxicity to a rat intestinal epithelial cell line
21. Distinct functions of protein kinase C-α and protein kinase C-β during retinoic acidinduced differentiation of F9 cells
22. 1 integrin
23. Cellular variability in the development of tight junction after activation of protein kinase C
24. Microtubule organization and its involvement in the biogenic pathways of plasma membrane proteins in Caco-2 intestinal epithelial cells
25. Immunocytochemical localization of eight PKC isoenzymes overexpressed in NIH 3T3 fibroblasts
26. Protein kinase C signaling and oxidative stress
27. Critical values for Dunnett's new multiple range test
28. MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin
29. Inflammatory bowel disease and adenomas in mice expressing a dominant negative N-cadherin
30. Crohn's disease - A permeability disorder of the tight junction?
31. The intestinal permeability barrier: A hypothesis as to its regulation and involvement in Crohn's disease
32. Overproduction of protein kinase C causes disordered growth control in rat fibroblasts
33. Permeability enhancement in Caco-2 cell monolayers by sodium salicylate and sodium taurodihydrosulfate: Assessment of effect-reversibility and imaging of transepithelial transport routes by laser confocal microscopy
34. Protein kinase C activation during corneal endothelial wound repair
35. Poly (ADP-ribose) synthetase activation mediates increased permeability induced by peroxynitrite in Caco-2BBe cells
36. Leaky gut in alcoholic cirrhosis: A possible mechanism for alcohol induced liver damage
37. Excessive production of reactive oxygen metabolites by inflamed colon: Analysis by chemiluminescence probe
38. Increased expression of an inducible isoform of nitric oxide synthase and the formation of peroxynitrite in colonic mucosa of patients with active ulcerative colitis
39. Towards an understanding of microtubule function and cell organization: An overview
40. Increased transforming growth factor-α levels in human colon carcinoma cell lines over-expressing protein kinase C
41. Evidence for oxidant-induced injury to epithelial cells during inflammatory bowel disease
42. The alpha isoform of protein kinase C inhibits histamine-stimulated adenylate cyclase activity in a particulate fraction of the human gastric cancer cell line HGT-1
43. Introduction of the β isozyme of protein kinase C accelerates induced differentiation of murine erythroleukemia cells
44. The human intestinal epithelial cell line Caco-2: Pharmacological and pharmacokinetics applications
45. Overexpression of protein kinase C-δ and -ε in NIH 3T3 cells induces opposite effects on growth morphology, anchorage dependence, and tumorigenicity
46. Overexpression of protein kinase C-δ increases tight junction permeability in LLC-PK 1 cells
47. Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C. The role of protein kinase C in cell surface signal transduction and tumor promotion
48. Altered growth regulation and enhanced tumorigenicity of NIH 3T3 fibroblasts transfected with protein kinase C cDNA
49. Protein kinase C isoenzymes in human neuroblasts: Involvement of PKC-α in cell differentiation
50. Differential induction of phosphotidylcholine hydrolysis, diacylglycerol formation and protein kinase C activation by EGF and TGF-α in normal human skin fibroblasts and keratinocytes
51. Assessment of inflammatory events in epithelial permeability: A rapid screening method using fluorescein dextrans
52. Activation of protein kinase C is associated with post-mitotic events in intestinal epithelial cells in situ
53. Helicobacter pylori disrupts epithelial barrier function in a process inhibited by protein kinase C activator
54. PKC-dependent regulation of transepithelial resistance: Roles of MLC and MLC kinase
55. Hyperpermeability of intestinal epithelial monolayers induced by NO: Effect of low extracellular pH
56. Thrombin and phorbol myristate acetate stimulate cytoskeletal polymerization in quiescent cells: A potential link to mitogenesis
57. Epidermal growth factor inhibits carbachol stimulated canine parietal cell function via protein kinase C
58. Mucosal injury and inflammation in a model of chronic granulomatous colitis in rats
59. Stimulation of PLD by EGF requires protein kinase C activation in Swiss 3T3 cells
60. Defective microtubule reorganization in phorbol ester-resistant U937 variant: Reconstitution of the normal cell phenotype with nocodazole treatment