The use of molecular beacons combined with NASBA for the sensitive detection of Sugarcane yellow leaf virus

European Journal of Plant Pathology

Volumn 108, Issue 5, 2002, Pages 401-407

  Gonccalves M C ^a   Klerks, M M ^b   Verbeek, M ^b   Vega, J ^c   Van Den Heuvel, J F J M ^d  

^a CEIB   (Brazil)

^b WAGENINGEN UNIVERSITY   (Netherlands)

^c UNIVERSITY OF CAMPINAS   (Brazil)

^d De Ruiter Seeds   (Netherlands)

Author keywords

Fluorescent probes; Isothermal amplification; Melanaphis sacchari; Real time detection; RT PCR; Sugarcane viruses

Indexed keywords

DETECTION METHOD; POLYMERASE CHAIN REACTION; SUGAR CANE; VIRAL DISEASE;

APHIDIDAE; ARUNDINARIA; LUTEOVIRIDAE; MELANAPHIS SACCHARI; SACCHARUM; SACCHARUM HYBRID CULTIVAR; SUGARCANE YELLOW LEAF VIRUS;

EID: 0036073353     PISSN: 09291873     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1016040314260     Document Type: Article

References (19)

1. Double-stranded RNA associated with sugarcane yellow leaf syndrome
2. Incidence of yellow leaf syndrome in CP cultivars based on polymerase chain reaction and serological techniques
3. Molecular beacons: A new approach to plant virus detection
4. NASBA isothermal enzymatic in vitro nucleic acid amplification optimized for HIV-1 diagnosis
5. Development of a multiplex AmpliDet RNA for the simultaneous detection of Potato leafroll virus and Potato virus Y in potato tubers
6. Rapid and sensitive detection of Apple stem pitting virus in apple trees through RNA amplification and probing with fluorescent molecular beacons
7. Detection of nematode-transmitted nepoviruses by the novel, one-tube AmpliDet RNA assay
8. Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA
9. Aphid transmission of a virus associated with sugarcane yellow leaf disease
10. Molecular evidence that Sugarcane yellow leaf virus (ScYLV) is a member of the Luteoviridae family
11. Sugarcane yellow leaf virus: An emerging virus that has evolved by recombination between Luteoviral and Poleroviral ancestors
12. Detection of Brazilian sugarcane yellow leaf luteovirus by DAS-ELISA using polyclonal antibodies
13. Transmission, characterization and serology of sugarcane yellow leaf luteovirus
14. Use of a tissue blot immunoassay to determine the distribution of sugarcane yellow leaf virus in Hawaii
15. Sugarcane yellow leaf virus: A novel member of the Luteoviridae that probably arose by interspecies recombination
16. Development of a multiplex AmpliDet RNA assay for simultaneous detection and typing of Potato virus Y isolates
17. Molecular beacons: Probes that fluoresce upon hybridization
18. Transmission of potato leafroll virus from plants and artificial diets
19. Sugarcane yellow leaf disease in Brazil: Evidence of association with a luteovirus