Development of a multiplex AmpliDet RNA assay for simultaneous detection and typing of potato virus Y isolates

Journal of Virological Methods

Volumn 100, Issue 1-2, 2002, Pages 83-96

  Szemes, M ^a,b   Klerks, M M ^a   Van Den Heuvel, J F J M ^c   Schoen, C D ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b Institute for Biotechnology Szeged   (Hungary)

^c De Ruiter Vegetable Seeds CV   (Netherlands)

Author keywords

AmpliDet RNA; Molecular beacons; NASBA; Potato virus Y; Strain differentiation

Indexed keywords

COAT PROTEIN;

ARTICLE; ASSAY; DIAGNOSTIC PROCEDURE; GENE AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POTATO; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; STRAIN DIFFERENCE; VALIDATION PROCESS; VIRUS DETECTION; VIRUS ISOLATION; VIRUS STRAIN; VIRUS TYPING;

BASE SEQUENCE; DNA, VIRAL; MOLECULAR SEQUENCE DATA; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POTYVIRUS; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY, NUCLEIC ACID;

POTATO VIRUS Y; SOLANUM TUBEROSUM;

EID: 0036136266     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(01)00402-5     Document Type: Article

References (27)

1. Studies on the etiology of tuber necrotic ringspot disease in potato
2. Potato virus Y from petunia can cause symptoms of potato tuber necrotic ringspot disease (PTNRD)
3. Tobacco veinal necrosis determinants are unlikely to be located within the 5′ and 3′ terminal sequences of the potato virus Y genome
4. Nucleic acid sequence-based amplification
5. Potato virus Y
6. Polymerase recognition of synthetic oligodeoxyribonucleotides incorporating degenerate pyrimidine and purine bases
7. NASBA isothermal enzymatic in vitro nucleic acid amplification optimized for HIV-1 diagnosis
8. A molecular beacon probe combined with amplification by NASBA enables homogeneous, real-time detection of RNA
9. Biological characterization of various geographical isolates of potato virus Y inducing superficial necrosis on potato tubers
10. Synthesis and duplex stability of oligonucleotides containing cytosine-thymine analogues
11. Comparative evaluation of hepatitis C virus RNA quantitation by branched DNA, NASBA, and monitor assays
12. Predicting oligonucleotide affinity to nucleic acid targets
13. NTN
14. The reactions of certain solanaceous species to strains of potato virus Y
15. TREEVIEW: An application to display phylogenetic trees on personal computers
16. Frequent occurrence of recombinant potyvirus isolates
17. A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics
18. O)
19. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
20. Molecular beacons: Probes that fluoresce upon hybridization
21. Multicolor molecular beacons for allele discrimination
22. Characteristics of a resistance-breaking isolate of potato virus Y causing tuber necrotic disease
23. Detection of different strains of potato virus Y and their mixed infections using competitive fluorescent RT-PCR
24. NTN) by reverse transcription and immunocapture polymerase chain reaction
25. N by a 3-primer PCR
26. A burgonya Y vírus (Potato Y Potyvirus, PVY) törzseinek elofordulása burgonya termoterületeken magyarországon