Use of both 16S rRNA and engineered functional genes with real-time PCR to quantify an engineered, PCB-degrading Rhodococcus in soil

Journal of Microbiological Methods

Volumn 51, Issue 2, 2002, Pages 181-189

  Rodrigues, Jorge L M ^a   Aiello, Michael R ^a,b   Urbance, John W ^a   Tsoi, Tamara V ^a   Tiedje, James M ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

^b NONE   (United States)

Author keywords

PCB degradation; Real time PCR; Rhodococcus strain RHA1(fcb); Soil

Indexed keywords

4 CHLOROBENZOIC ACID; OLIGONUCLEOTIDE; POLYCHLORINATED BIPHENYL; RNA 16S;

ARTICLE; BIOENGINEERING; COLONY FORMING UNIT; CONTROLLED STUDY; DNA PROBE; FLUORESCENCE; MOLECULAR DYNAMICS; NONHUMAN; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RHODOCOCCUS; SOIL DEGRADATION;

BIODEGRADATION, ENVIRONMENTAL; COLONY COUNT, MICROBIAL; DNA PRIMERS; DNA PROBES; GENETIC ENGINEERING; POLYMERASE CHAIN REACTION; RHODOCOCCUS; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SOIL MICROBIOLOGY;

RHODOCOCCUS; RHODOCOCCUS SP.;

EID: 0036067454     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(02)00067-2     Document Type: Article

References (30)

1. The oligonucleotide probe database
2. Basic local alignment search tool
3. Use of fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes
4. Contamination and sensitivity issues with a real time universal 16S rRNA PCR
5. Comparison of the ABI 7700 system (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of tuberculosis
6. Pseudomonas stutzeri nitrite reductase gene abundance in environmental samples measured by real-time PCR
7. Collection of small subunit (16S- and 16S-like) ribosomal RNA structures
8. Rhodococcus marinonascens sp. nov., an actinomycete from the sea
9. 5′ Nuclease PCR assay to detect Yersinia pestis
10. Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions
11. RRNA operon copy number reflects ecological strategies of bacteria
12. Proviral load determination of different feline immunodeficiency virus using real-time polymerase chain reaction: Influence of mismatches on quantification
13. 16S rRNA gene-based detection of tetrachloroethene-dechlorinating Desulforomonas and Dehalococcoides species
14. A new version of the RDP (Ribosomal Database Project)
15. Degradation of anaerobic reductive dechlorination products of Arochlor 1242 by four aerobic bacteria
16. The extraction and purification of microbial DNA from sediments
17. Quantitative detection of Borrelia burgdorferi by real-time PCR
18. Development of a Rhodococcus recombinant strain for degradation of products from anaerobic dechlorination of PCBs
19. The neighbor-joining method: A new method for reconstructing phylogenetic trees
20. Partial and complete 16S rDNA sequences, their use in generation of 16S rDNA phylogenetic trees and their implications in molecular ecological studies
21. Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5′-nuclease assays
22. Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast
23. Cloning, expression, and nucleotide sequence of the Pseudomonas aeruginosa 142 ohb genes coding for oxygenolytic ortho dehalogenation of halobenzoates
24. Genome conformation and genetic diversity: A closer look at several species of Rhodococcus isolated from contaminated soils
25. Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification
26. Direct DNA extraction for PCR-mediated assays of soil organisms
27. 16S ribosomal DNA amplification for phylogenetic study
28. Genetic control of degradation of chlorinated benzoic acids in Arthrobacter globiformis, Corynebacterium sepedonicum, and Pseudomonas cepacia strains