In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I

European Journal of Biochemistry

Volumn 269, Issue 16, 2002, Pages 3969-3977

  Zheng, Feng ^a   Angleton, Eddie L ^a   Lu, Jin ^a   Peng, Sheng Bin ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

Inactivation; Regulation; Self cleavage; Signal peptidase I; Streptococcus pneumoniae

Indexed keywords

ALANINE; BACTERIAL ENZYME; GLYCINE; HISTIDINE; PHENYLALANINE; PHOSPHOLIPID; SIGNAL PEPTIDASE I;

ARTICLE; CELL GROWTH; CHEMICAL ANALYSIS; CONTROLLED STUDY; ENZYME DEGRADATION; ENZYME INACTIVATION; GROWTH CURVE; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; STREPTOCOCCUS PNEUMONIAE;

AMINO ACID SEQUENCE; ANTIBODIES, BACTERIAL; BACTERIAL PROTEINS; CATALYSIS; ENZYME PRECURSORS; KINETICS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PHOSPHOLIPIDS; RECOMBINANT FUSION PROTEINS; SEQUENCE DELETION; SERINE ENDOPEPTIDASES; STREPTOCOCCUS PNEUMONIAE; STREPTOKINASE; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS PNEUMONIAE;

EID: 0036037289     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.03083.x     Document Type: Article

References (41)

1. The enzymology of protein translocation across the Escherichia coli plasma membrane
2. The chemistry and enzymology of type I signal peptidase
3. The structure and mechanism of bacterial type I signal peptidases, a novel antibiotic target
4. Nucleotide sequence of the Escherichia coli prolipoprotein signal peptidase (lsp) gene
5. The Sec system
6. Following the leader: Bacterial protein export through the Sec pathway
7. A small hydrophobic domain anchors leader peptidase to the cytoplasmic membrane of Escherichia coli
8. Use of phoA fusions to study the topology of Escherichia coli inner membrane protein leader peptidase
9. Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in bacterial envelope
10. Leader peptidase catalyzes the release of exported proteins from the outer surface of the Escherichia coli plasma membrane
11. Export of unprocessed precursor maltose-binding protein to the periplasma of Escherichia coli cells
12. Diverse effects of mutations in the signal sequence on the secretion of beta-lactamase in Salmonella typhimurium
13. Conserved residues of the leader peptide are essential for cleavage by leader peptidase
14. Bacterial leader peptidase I
15. Evidence that the catalytic activity of prokaryote leader peptidase depends upon the operation of a serine-lysine catalytic dyad
16. A serine and a lysine residue implicated in the catalytic mechanism of the Escherichia coli leader peptidase
17. Identification of the potential active site of the signal peptidase SipS of Bacillus subtilis. Structural and functional similarities with LexA-like proteases
18. Biochemical characterization of signal peptidase I from Gram-positive Streptococcus pneumoniae
19. On the catalytic mechanism of prokaryotic leader peptidase I
20. Crystal structure of a bacterial signal peptidase in complex with a beta-lactam inhibitor
21. LexA cleavage and other self-processing reactions
22. Structure of UmuD′ protein and its regulation in response to DNA damage
23. Reactions of LexA repressor with diisopropyl fluorophosphate. A test of the serine protease model
24. Intermolecular degradation of signal peptidase I in vitro
25. A truncated soluble Bacillus signal peptidase produced in Escherichia coli is subject to self-cleavage at its active site
26. The streptokinase gene of group A streptococci: Cloning, expression in Escherichia coli, and sequence analysis
27. Development of an internally quenched fluorescent substrate and a continuous fluorimetric assay for Streptococcus pneumoniae signal peptidase I
28. Analysis of a Streptococcus pneumoniae gene encoding signal peptidase I and overproduction of the enzyme
29. Use of T7 RNA polymerase to direct expression of cloned genes
30. Identification of a 14-Kda subunit associated with the catalytic sector of Clathrin-coated vesicle H+-ATPase
31. Patterns of amino acids near signal-sequence cleavage sites
32. How signal sequences maintain cleavage specificity
33. A new method for predicting signal sequence cleavage sites
34. 16S rRNA is bound to Era of the Streptococcus pneumoniae
35. Molecular cloning of the Salmonella typhimurium lep gene in Escherichia coli
36. Whole genome random sequencing and assembly of Haemophilus influenzae
37. Molecular cloning and expression of the spsB gene encoding an essential type I signal peptidase from Staphylococcus aureus
38. The endogenous B. subtilis plasmids pTA1015 and pTA1040 contain signal peptidase-encoding genes. Identification of a new structural module on cryptic plasmids
39. Bacillus subtilis contains four closely related type I signal peptidase with overlapping substrate specificities
40. Signal peptidase I of B. subtilis: Patterns of conserved amino acids in prokaryotic and eukaryotic type I signal peptidases
41. Four genes encoding different type I signal peptidase are organized in a cluster in Streptomyces lividans TK21