Use of degenerate primers and touchdown PCR to amplify a halogenase gene fragment from Streptomyces venezuelae ISP5230

Journal of Industrial Microbiology and Biotechnology

Volumn 29, Issue 1, 2002, Pages 1-5

  Piraee, M ^a   Vining, L C ^a  

^a DALHOUSIE UNIVERSITY   (Canada)

Author keywords

FAD binding site; Gene cloning; Halometabolite; PCR

Indexed keywords

DNA FRAGMENT; ENZYME; FLAVINE ADENINE NUCLEOTIDE; HALOGENASE; PRIMER DNA; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE; OXIDOREDUCTASE;

ACTINOBACTERIA; AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CONSENSUS SEQUENCE; ENZYME ACTIVITY; GENE AMPLIFICATION; GENE INSERTION; GENE ISOLATION; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PROTEIN FOLDING; SEQUENCE HOMOLOGY; STREPTOMYCES AUREOFACIENS; STREPTOMYCES COELICOLOR; STREPTOMYCES VENEZUELAE; BACTERIAL GENE; CHEMISTRY; CLASSIFICATION; ENZYMOLOGY; GENETICS; METHODOLOGY; MOLECULAR GENETICS; SENSITIVITY AND SPECIFICITY; SOUTHERN BLOTTING; STREPTOMYCES;

AMINO ACID SEQUENCE; BLOTTING, SOUTHERN; DNA PRIMERS; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY, AMINO ACID; STREPTOMYCES; SUPPORT, NON-U.S. GOV'T;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); INSERTION SEQUENCES; NEGIBACTERIA; STREPTOMYCES; STREPTOMYCES AUREOFACIENS; STREPTOMYCES COELICOLOR; STREPTOMYCES VENEZUELAE; STREPTOMYCINEAE; UNCULTURED ACTINOMYCETE;

EID: 0036023485     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.jim.7000263     Document Type: Article

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