Cultivation of primary porcine hepatocytes in an OXY-HFB for use as a bioartificial liver device

Biotechnology Progress

Volumn 18, Issue 4, 2002, Pages 839-846

  Jasmund, Inka ^a   Langsch, Angelika ^a   Simmoteit, Robert ^a   Bader, Augustinus ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DIAZEPAM; OXYGEN;

ANIMAL; ARTICLE; ARTIFICIAL LIVER; BIOREACTOR; CELL COUNT; CELL CULTURE; CELL SURVIVAL; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOLOGY; EQUIPMENT DESIGN; INSTRUMENTATION; LIVER CELL; METABOLISM; METHODOLOGY; PERFUSION; SWINE; TIME;

ANIMALS; BIOREACTORS; CELL COUNT; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CELLS, CULTURED; CULTURE MEDIA; DIAZEPAM; EQUIPMENT DESIGN; HEPATOCYTES; LIVER, ARTIFICIAL; OXYGEN; PERFUSION; SWINE; TIME FACTORS; ANIMAL; CELL CULTURE; SUPPORT, NON-U.S. GOV'T;

BIOARTIFICIAL DEVICES;

ARTIFICIAL ORGANS; BIOREACTORS; GLUCOSE; HEAT EXCHANGERS; METABOLISM; PH EFFECTS; SYNTHESIS (CHEMICAL);

BIOTECHNOLOGY;

SUIDAE;

EID: 0036022004     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp025501y     Document Type: Article

References (24)

1. In vitro morphological and functional characterization of isolated porcine hepatocytes for extracorporeal liver support: Bile acid uptake and conjugation
2. Bioartificial liver support: Developments in hepatocyte culture and bioreactor design
3. Manipulation of the phenotype of immortalised rat hepatocytes by different culture configurations and by dimethyl sulphoxide
4. Immobilisation and long-term albumin secretion of hepatocyte spheroids rapidly formed by rotational tissue culture methods
5. Matrix-induced liver cell aggregates (MILCA) for bioartificial liver use
6. Mass transfer limitations to the performance of membrane bioartificial liver support devices
7. Metabolism of diazepam in vitro by human liver. Independent variability of N-demethylation and C3-hydroxylation
8. Collagen substrata for studies on cell behavior
9. Hepatocyte function and extracellular matrix geometry: Long-term culture in a sandwich configuration
10. Diffusion in tissue cultures on gas-permeable and impermeable supports
11. Oxygen transfer in a diffusion-limited hollow fiber bioartificial liver
12. Development of a hybrid liver support system: A review
13. Mathematical modeling and analysis of glucose and glutamine utilization and regulation in cultures of continuous mammalian cells
14. Comparative analysis of glucose and glutamine metabolism in transformed mammalian cell lines, insect and primary liver cells
15. Improved hepatocyte in vitro maintenance in a culture model with woven multicompartment capillary systems: Electron microscopy studies
16. High cell-density culture system of hepatocytes entrapped in a three- dimensional hollow fiber module with collagen gel
17. In vivo evaluation of a hollow fiber liver assist device
18. Semipermeable hollow fiber membranes in hepatocyte bioreactors: A prerequisite for a successful bioartificial liver?
19. A novel full-scale fiat membrane bioreactor utilizing porcine hepatocytes: Cell viability and tissue-specific functions
20. In vitro evaluation of a novel bioreactor based on an integral oxygenator and a spirally wound nonwoven polyester matrix for hepatocyte culture as small aggregates
21. Treatment of acute liver failure: Hybrid liver support. A critical overview
22. Transfer of porcine endogenous retrovirus across hollow fiber membranes: Significance to a bioartificial liver
23. Significantly improved survival time in pigs with complete liver ischemia treated with a novel bioartificial liver