cAMP increases density of ENaC subunits in the apical membrane of MDCK cells in direct proportion to amiloride-sensitive Na+ transport

Journal of General Physiology

Volumn 120, Issue 1, 2002, Pages 71-85

  Morris, Ryan G ^a   Schafer, James A ^a,b  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b Department of Physiology and Biophysics ^*  (United States)

Author keywords

Channel number; FLAG epitope; Membrane trafficking; Retroviral transfection; Short circuit current

Indexed keywords

8 (4 CHLOROPHENYLTHIO) CYCLIC AMP; AMILORIDE; ANTIBODY; CYCLIC AMP; EPITOPE; IODINE 125; ISOBUTYLMETHYLXANTHINE; PROTEIN ENAC; PROTEIN SUBUNIT; SODIUM ION; UNCLASSIFIED DRUG; VASOPRESSIN; EPITHELIAL SODIUM CHANNEL; FLAG PEPTIDE; ISOPROTEIN; PEPTIDE; SODIUM; SODIUM CHANNEL;

ALPHA CHAIN; ANIMAL CELL; ANIMAL TISSUE; ANTIBODY LABELING; APICAL MEMBRANE; ARTICLE; BETA CHAIN; CELL STRAIN; CHANNEL GATING; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DENSITY; EPITHELIUM; GENETIC TRANSFECTION; KIDNEY COLLECTING TUBULE; MEMBRANE TRANSPORT; NONHUMAN; RAT; RETROVIRUS; SHORT CIRCUIT CURRENT; SODIUM TRANSPORT; ANIMAL; CELL LINE; CELL MEMBRANE; CYTOLOGY; DOG; DRUG EFFECT; ELECTRIC CONDUCTIVITY; KIDNEY; METABOLISM; PHYSIOLOGY; TRANSPORT AT THE CELLULAR LEVEL;

AMILORIDE; ANIMAL; BIOLOGICAL TRANSPORT; CELL LINE; CELL MEMBRANE; CYCLIC AMP; DOGS; ELECTRIC CONDUCTIVITY; KIDNEY; PEPTIDES; PROTEIN ISOFORMS; RATS; SODIUM; SODIUM CHANNELS; SUPPORT, U.S. GOV'T, P.H.S.; ANIMALS; EPITHELIAL SODIUM CHANNEL;

EID: 0036020208     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.20018547     Document Type: Article

References (45)

1. Regulation of epithelial sodium channels by short actin filaments
2. Stimulation of sodium transport by aldosterone and arginine vasotocin in A6 cells
3. + channel: Binding sites and channel densities
4. + channel complex from rat lymphocytes
5. Effects of vasopressin on electrolyte transport across isolated colon from normal and dexamethasone-treated rats
6. Expression and regulation of normal and polymorphic epithelial sodium channel by human lymphocytes
7. cAMP-sensitive endocytic trafficking in A6 epithelia
8. + channel is made of three homologous subunits
9. + reabsorption and C1-secretion by arginine vasopressin in the amphibian cell line A6
10. Design of retro-viral expression vectors
11. The heterotetrameric architecture of the epithelial sodium channel (ENaC)
12. Cell surface expression of the epithelial Na channel and a mutant causing Liddle syndrome: A quantitative approach
13. + channel
14. Epithelial sodium channels: Function, structure, and regulation
15. Immunocytochemical and immunoelectron microscopic localization of α-, β-, and γ-ENaC in rat kidney
16. 2+
17. Effects of phosphorylation on ion channel function
18. + channels by actin in planar lipid bilayers and in the Xenopus oo-cyte expression system
19. Defining topological similarities among ion transport proteins with anti-amiloride antibodies
20. + channels in A6 cells
21. Subunit stoichiometry of the epithelial sodium channel
22. + channels in mouse CCD cells
23. Effects of vasopressin and cAMP on single amiloride-blockable Na channels
24. Aldosterone-mediated regulation of ENaC α, β, and γ subunit proteins in rat kidney
25. Micro-tubule disruption inhibits an AVT-stimulated chloride secretion but not sodium reabsorption in A6 cells
26. + channels in planar lipid bilayers
27. + channels
28. Sodium transport by rat cortical collecting tubule. Effects of vasopressin and desoxycorticosterone
29. Trafficking and cell surface stability of ENaC
30. Salt and water homeostasis - Is it just a matter of good bookkeeping?
31. Abnormal regulation of ENaC: Syndromes of salt retention and salt wasting by the collecting duct
32. + channels in the cortical collecting duct by AVP and mineralocorticoids
33. + conductance produced in the rat CCD by vasopressin
34. + channel in oocyte expression system
35. CFTR regulates outwardly rectifying chloride channels through an autocrine mechanism involving ATP
36. + channel protein into planar lipid bilayers
37. In vivo phosphorylation of the epithelial sodium channel
38. + channel to the cell surface
39. Electrophysiological and biochemical evidence that DEG/ ENaC cation channels are composed of nine subunits
40. CFTR as a cAMP-dependent regulator of sodium channels
41. Control of sodium and potassium transport in the cortical collecting duct of the rat. Effects of bradykinin, vasopressin, and deoxycorticosterone
42. Membrane structural studies of the action of vasopressin
43. Non-coordinate regulation of endogenous epithelial sodium channel (ENaC) subunit expression at the apical membrane of A6 cells in response to various transporting conditions
44. The endocytotic rate constant. A cellular parameter for quantitating receptor-mediated endocytosis
45. Nongenomic regulation of ENaC by aldosterone