The amiloride-sensitive epithelial Na+ channel: Binding sites and channel densities

American Journal of Physiology - Cell Physiology

Volumn 272, Issue 3 41-3, 1997, Pages

  Blazer Yost, Bonnie L ^a   Helman, Sandy I ^b  

^a INDIANA UNIVERSITY   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

A6 cell line; frog skin; noise analysis; patch clamp; renal collecting duct; toad urinary bladder

Indexed keywords

AMILORIDE; SODIUM CHANNEL;

BINDING SITE; FLUID BALANCE; FROG; NONHUMAN; PRIORITY JOURNAL; REVIEW; SODIUM TRANSPORT; WATER TRANSPORT;

ANURA;

EID: 0030960807     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.1997.272.3.c761     Document Type: Review

References (90)

1. + permeability of tight epithelia. Noise analysis with amiloride and CGS 4270. J. Gen. Physiol. 85: 555-582, 1985.
2. 3H]benzamil. J. Physiol. (Lond.) 295: 477-490, 1979.
3. + channel protein displays stretch activation in planar lipid bilayers. Am. J. Physiol. 268 (Cell Physiol. 37): C1450-C1459, 1995.
4. Barbry, P., M. Champe, O. Chassande, S. Munemitsu, G. Champigny, E. Lingueglia, P. Maes, C. Frelin, A. Tartar, A. Ullrich, and M. Lazdunski. Human kidney amiloride-binding protein: cDNA structure and functional expression. Proc. Natl. Acad. Sci. USA 87: 7347-7351, 1990.
5. + channel. Proc. Natl. Acad. Sci. USA 84: 4836-4840, 1987.
6. Barbry, P., and M. Lazdunski. Structure and regulation of the amiloride-sensitive epithelial sodium channel. In: Ion Channels, edited by T. Narahashi. New York: Plenum, 1996, vol. 4, p. 115-167.
7. Benos, D. J., and L. J. Mandel. Irreversible inhibition of sodium entry sites in frog skin by a photosensitive amiloride analog. Science 199: 1205-1206, 1978.
8. Benos, D. J., G. Saccomani, and S. Sariban-Sohraby. The epithelial sodium channel. Subunit number and location of the amiloride binding site. J. Biol. Chem. 262: 10613-10618, 1987.
9. Bentley, P. J. Amiloride: a potent inhibitor of sodium transport across the toad bladder. J. Physiol. (Lond.) 195: 317-330, 1968.
10. + channels (Abstract). FASEB J. 8: A294, 1994.
11. Breuer, W., H. Glickstein, N. Kartner, J. R. Riordan, D. A. Ausiello, and I. Z. Cabantchik. Protein kinase C mediates down-regulation of cystic fibrosis transmembrane conductance regulator levels in epithelial cells. J. Biol. Chem. 268: 13935-13939, 1993.
12. + channels in rat kidney medulla. Am. J. Physiol. 256 (Renal Fluid Electrolyte Physiol. 25): F366-F369, 1989.
13. + channel is made of three homologous subunits. Nature 367: 463-466, 1994.
14. + channel from the epithelial cell line, A6. J. Biol. Chem. 264: 20867-20870, 1989.
15. 2 and lipoxygenase pathways. J. Biol. Chem. 265: 21624-21628, 1990.
16. Christensen, O., and N. Bindslev. Fluctuation analysis of short-circuit current in a warm-blooded sodium-retaining epithelium: site current, density and interaction with triamterene. J. Membr. Biol. 65: 19-30, 1982.
17. Cuthbert, A. W. An upper limit to the number of sodium channels in frog skin epithelium. J. Physiol. (Lond.) 228: 681-692, 1973.
18. Cuthbert, A. W. Characteristics of sodium channels in transporting epithelia. In: Drugs and Transport Processes, edited by B. A. Callingham. Baltimore, MD: University Park Press, 1974, p. 173-189.
19. Cuthbert, A. W., and J. M. Edwardson. Benzamil binding to kidney cell membranes. Biochem. Pharmacol. 30: 1175-1183, 1981.
20. + in the hen colon and coprodaeum. Pflügers Arch. 392: 347-351, 1982.
21. Cuthbert, A. W., and W. K. Shum. Effects of vasopressin and aldosterone on amiloride binding in toad bladder epithelial cells. Proc. R. Soc. Lond. 189: 543-575, 1975.
22. Cuthbert, A. W., and W. K. Shum. Induction of transporting sites in a sodium transporting epithelium. J. Physiol. (Lond.) 260: 213-235, 1976.
23. Cuthbert, A. W., and W. K. Shum. Characteristics of the entry process for sodium in transporting epithelia as revealed with amiloride. J. Physiol. (Lond.) 255: 587-604, 1976.
24. Cuthbert, A. W., and W. K. Shum. Estimation of the lifespan of amiloride binding sites in the membranes of toad bladder epithelial cells. J. Physiol. (Lond.) 255: 605-618, 1976.
25. Das, S., M. Garepapaghi, and L. G. Palmer. Stimulation by cGMP of apical Na channels and cation channels in toad urinary bladder. Am. J. Physiol. 260 (Cell Physiol. 29): C234-C241, 1991.
26. Duchatelle, P., A. Ohara, B. N. Ling, A. E. Kemendy, K. E. Kokko, P. S. Matsumoto, and D. C. Eaton. Regulation of renal epithelial sodium channels. Mol. Cell. Biochem. 114: 27-34, 1992.
27. Eaton, D. C., A. Becchetti, H. P. Ma, and B. N. Ling. Renal sodium channels: regulation and single channel properties. Kidney Int. 48: 941-949, 1995.
28. Eaton, D. C., and Y. Marunaka. Ion channel fluctuations: "noise" and single-channel measurements. In: Channels and Noise in Epithelial Tissues, edited by S. I. Helman and W. Van Driessche. New York: Academic, 1990, p. 61-113. (Curr. Top. Membr. Transp. vol. 37)
29. Eaton, D. C., and Y. Marunaka. Ion channel fluctuations: "noise" and single-channel measurements. In: Channels and Noise in Epithelial Tissues, edited by S. I. Helman and W. Van Driessche. New York: Academic, 1990, p. 61-113. (Curr. Top. Membr. Transp. vol. 37)
30. Eigler, J., and J. Crabbé. Effect of diuretics on active Na-transport in amphibian membranes. In: Renal Transport and Diuretics, edited by K. Thurau and H. Jahrmarker. Berlin: Springer-Verlag, 1969, p. 195-208.
31. 2, and indomethacin on active Na transport in frog skin: studies with microelectrodes. Am. J. Physiol. 241 (Renal Fluid Electrolyte Physiol. 10): F279-F288, 1981.
32. Els, W. J., and S. I. Helman. Activation of epithelial Na channels by hormonal and autoregulatory mechanisms of action. J. Gen. Physiol. 98: 1197-1220, 1991.
33. + channels in frog skin (R. pipiens). J. Membr. Biol. 155: 75-87, 1997.
34. Fisher, R. S., D. Erlij, and S. I. Helman. Intracellular voltage of isolated epithelia of frog skin. Apical and basolateral cell punctures. J. Gen. Physiol. 76: 447-453, 1980.
35. Frindt, G., R. B. Silver, E. E. Windhager, and L. G. Palmer. Feedback regulation of Na channels in rat CCT. III. Response to cAMP. Am. J. Physiol. 268 (Renal Fluid Electrolyte Physiol. 37): F480-F489, 1995.
36. Frings, S. M., R. D. Purves, and A. D. C. Macknight. Ion channels in urinary bladder. Renal Physiol. Biochem. 13: 112-128, 1990.
37. + permeability by aldosterone. Semin. Nephrol. 12: 24-29, 1992.
38. Garvin, J. L., S. A. Simon, E. J. Cragoe, Jr., and L. J. Mandel. Phenamil: an irreversible inhibitor of sodium channels in the toad urinary bladder. J. Membr. Biol. 87: 45-54, 1985.
39. 3H-phenamil, an irreversible amiloride analog, to toad urinary bladder: effects of aldosterone and vasopressin. J. Membr. Biol. 90: 107-113, 1986.
40. + channel. J. Biol. Chem. 268: 7856-7862, 1993.
41. 2 and G proteins in rat medullary thick ascending limb. Am. J. Physiol. 270 (Renal Fluid Electrolyte Physiol. 39): F711-F717, 1996.
42. Granitzer, M., T. Leal, W. Nagel, and J. Crabbe. Apical and basolateral conductance in cultured A6 cells. Pflügers Arch. 417: 463-468, 1991.
43. Granitzer, M., W. Nagel, and J. Crabbé. Basolateral membrane conductance in A6 cells: effect of high sodium transport rate. Pflügers Arch. 420: 559-565, 1992.
44. Hamilton, K. L., and D. C. Eaton. Single-channel recordings from amiloride-sensitive epithelial sodium channel. Am. J. Physiol. 249 (Cell Physiol. 18): C200-C207, 1985.
45. + channels. Membr. Biochem. 6: 149-171, 1986.
46. Helman, S. I., and L. M. Baxendale. Blocker-related changes of channel density. Analysis of a three-state model for apical Na channels of frog skin. J. Gen. Physiol. 95: 647-678, 1990.
47. Helman, S. I., T. C. Cox, and W. Van Driessche. Hormonal control of apical membrane transport in epithelia. Studies with fluctuation analysis. J. Gen. Physiol. 82: 201-220, 1983.
48. Helman, S. I., and R. S. Fisher. Microelectrode studies of the active Na transport pathway of frog skin. J. Gen. Physiol. 69: 571-604, 1977.
49. Helman, S. I., and N. L. Kizer. Apical sodium ion channels of tight epithelia as viewed from the perspective of noise analysis. In: Channels and Noise in Epithelial Tissues, edited by S. I. Helman and W. Van Driessche. New York: Academic, 1990, p. 117-155. (Curr. Top. Membr. Transp. vol. 37)
50. Helman, S. I., and N. L. Kizer. Apical sodium ion channels of tight epithelia as viewed from the perspective of noise analysis. In: Channels and Noise in Epithelial Tissues, edited by S. I. Helman and W. Van Driessche. New York: Academic, 1990, p. 117-155. (Curr. Top. Membr. Transp. vol. 37)
51. Helman, S. I., B. M. Koeppen, K. W. Beyenbach, and L. M. Baxendale. Patch clamp studies of apical membranes of renal cortical collecting ducts. Pflügers Arch. 405, Suppl. 1: S71-S76, 1985.
52. Helman, S. I., W. Nagel, and R. S. Fisher. Ouabain on active transepithelial sodium transport in frog skin. Studies with microelectrodes. J. Gen. Physiol. 74: 105-127, 1979.
53. Helman, S. I., and S. M. Thompson. Interpretation and use of electrical equivalent circuits in studies of epithelial tissues. Am. J. Physiol. 243 (Renal Fluid Electrolyte Physiol. 12): F519-F531, 1982.
54. Helman, S. I., and W. Van Driessche (Editors). Channels and Noise in Epithelial Tissues. New York: Academic, 1990, p. 1-318. (Curr. Top. Membr. Transp. vol. 37)
55. Hinton, C. F., and D. C. Eaton. Expression of the amiloride-blockable sodium channels in Xenopus oocytes. Am. J. Physiol. 257 (Cell Physiol. 26): C825-C829, 1989.
56. Kemendy, A. E., T. R. Kleyman, and D. C. Eaton. Aldosterone alters the open probability of amiloride-blockable sodium channels in A6 epithelia. Am. J. Physiol. 263 (Cell Physiol. 32): C825-C837, 1992.
57. Kleyman, T. R., and E. J. Cragoe, Jr. Amiloride and its analogs as tools in the study of ion transport. J. Membr. Biol. 105: 1-21, 1988.
58. + channels in the amphibian cell line A6 is not altered by mineralocorticoids. Anaylsis using a new photoactive amiloride analog in combination with anti-amiloride antibodies. J. Biol. Chem. 264: 11995-12000, 1989.
59. + channel antibody raised by an antiidiotypic route. J. Biol. Chem. 266: 3907-3915, 1991.
60. Kleyman, T. R., T. Yulo, C. Ashbaugh, D. Landry, E. J. Cragoe, Jr., A. Karlin, and Q. Al-Awqati. Photoaffinity labeling of the epithelial sodium channel. J. Biol. Chem. 261: 2839-2843, 1986.
61. Koefoed-Johnsen, V., and H. H. Ussing. The nature of the frog skin potential. Acta Physiol. Scand. 42: 298-308, 1958.
62. Lindemann, B., and W. Van Driessche. Sodium-specific channels of frog skin are pores: current fluctuations reveal high turnover. Science 195: 292-294, 1977.
63. Ling, B. N., C. F. Hinton, and D. C. Eaton. Amiloride-sensitive sodium channels in rabbit cortical collecting tubule primary cultures. Am. J. Physiol. 261 (Renal Fluid Electrolyte Physiol. 30): F933-F944, 1991.
64. Ling, B. N., A. E. Kemendy, K. E. Kokko, C. F. Hinton, Y. Marunaka, and D. C. Eaton. Regulation of the amiloride-blockable sodium channel from epithelial tissue. Mol. Cell. Biochem. 99: 141-150, 1990.
65. Lingueglia, E., S. Renard, N. Voilley, R. Waldmann, O. Chassande, M. Lazdunski, and P. Barbry. Molecular cloning and functional expression of different molecular forms of rat amiloride-binding protein. Eur. J. Biochem. 216: 679-687, 1993.
66. + channel. Exp. Physiol. 81: 483-492, 1996.
67. + channel: a new channel type with homologies to Caenorhabditis elegans degenerins. FEBS Lett. 318: 95-99, 1993.
68. Marunaka, Y., and D. C. Eaton. Effects of vasopressin and cAMP on single amiloride-blockable Na channels. Am. J. Physiol. 260 (Cell Physiol. 29): C1071-C1084, 1991.
69. Nagel, W. The dependence of the electrical potentials across the membranes of the frog skin upon concentration of sodium in the mucosal solution. J. Physiol. (Lond.) 269: 777-796, 1977.
70. Novotny, W. F., O. Chassande, M. Baker, M. Lazdunski, and P. Barbry. Diamine oxidase is the amiloride binding protein and is inhibited by amiloride analogues. J. Biol. Chem. 269: 9921-9925, 1994.
71. + channel in planar lipid bilayers. Am. J. Physiol. 264 (Cell Physiol. 33): C1489-C1499, 1993.
72. O'Neil, R. G., and E. L. Boulpaep. Effect of amiloride on the apical cell membrane cation channels of a sodium-absorbing, potassium-secreting renal epithelium. J. Membr. Biol. 50: 365-387, 1979.
73. 69a. Pácha, J., G. Frindt, L. Antonian, R. B. Silver, and L. G. Palmer. Regulation of Na channels of the rat cortical collecting tubule by aldosterone. J. Gen. Physiol. 102: 25-42, 1993.
74. Palmer, L. G. Epithelial Na channels: the nature of the conducting pore. Renal Physiol. Biochem. 13: 51-58, 1990.
75. Palmer, L. G. Epithelial Na channels: function and diversity. Annu. Rev. Physiol. 54: 51-66, 1992.
76. Palmer, L. G. Epithelial Na channels and their kin. News Physiol. Sci. 10: 61-67, 1995.
77. Palmer, L. G., I. Corthesy-Theulaz, H.-P. Gaeggeler, J.-P. Kraehenbuhl, and B. Rossier. Expression of epithelial Na channels in Xenopus oocytes. J. Gen. Physiol. 96: 23-46, 1990.
78. Palmer, L. G., and G. Frindt. Amiloride-sensitive Na channels from the apical membrane of the rat cortical collecting tubule. Proc. Natl. Acad. Sci. USA 83: 2767-2770, 1986.
79. Palmer, L. G., and G. Frindt. Conductance and gating of epithelial Na channels from rat cortical collecting tubule. Effects of luminal Na and Li. J. Gen. Physiol. 92: 121-138, 1988.
80. Palmer, L. G., J. H.-Y. Li, B. Lindemann, and I. S. Edelman. Aldosterone control of the density of sodium channels in the toad urinary bladder. J. Membr. Biol. 64: 91-102, 1982.
81. Perkins, F. M., and J. S. Handler. Transport properties of toad kidney epithelia in culture. Am. J. Physiol. 241 (Cell Physiol. 10): C154-C159, 1981.
82. + channels by protein kinase A requires actin filaments. Am. J. Physiol. 265 (Cell Physiol. 34): C224-C233, 1993.
83. Puoti, A., A. May, C. M. Canessa, J.-D. Horisberger, L. Schild, and B. C. Rossier. The highly selective low-conductance epithelial Na channel of Xenopus laevis A6 kidney cells. Am. J. Physiol. 269 (Cell Physiol. 38): C188-C197, 1995.
84. Sariban-Sohraby, S., and D. J. Benos. Detergent solubilization, functional reconstitution, and partial purification of epithelial amiloride-binding protein. Biochemistry 25: 4639-4646, 1986.
85. + channels reconstituted into planar lipid bilayer membranes. Nature 308: 80-82, 1984.
86. + conductance in maturing rabbit principal cell. Am. J. Physiol. 270 (Renal Fluid Electrolyte Physiol. 39): F391-F397, 1996.
87. Soltoff, S. P., E. J. Cragoe, Jr., and L. J. Mandel. Amiloride analogues inhibit proximal tubule metabolism. Am. J. Physiol. 250 (Cell Physiol. 19): C744-C747, 1986.
88. Sorscher, E. J., M. A. Accavitti, D. Keeton, E. Steadman, R. A. Frizzell, and D. J. Benos. Antibodies against purified epithelial sodium channel protein from bovine renal papilla. Am. J. Physiol. 255 (Cell Physiol. 24): C835-C843, 1988.
89. Tousson, A., C. D. Alley, E. J. Sorscher, B. R. Brinkley, and D. J. Benos. Immunochemical localization of amiloride-sensitive sodium channels in sodium-transporting epithelia. J. Cell Sci. 93: 349-362, 1989.
90. Ussing, H. H., and K. Zerahn. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin. Acta Physiol. Scand. 23: 110-127, 1951.