Imaging secretory vesicles by fluorescent protein insertion in propeptide rather than mature secreted peptide

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Volumn 3, Issue 7, 2002, Pages 461-471

  Watkins, Simon ^a   Geng, Xuehui ^a   Li, Lehong ^a   Papworth, Glenn ^a   Robbins, Paul D ^a   Drain, Peter ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Diabetes; Exocytosis; Granule; Kinetic imaging; Neuropeptide; Proinsulin; Secretion; Secretory peptide trafficking; Secretory vesicle

Indexed keywords

C PEPTIDE; GLIBENCLAMIDE; GLUCOSE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; INSULIN; INSULIN ANTIBODY; NEUROPEPTIDE; PEPTIDE HORMONE; PROINSULIN; RED FLUORESCENT PROTEIN; SECRETORY PROTEIN; PHOTOPROTEIN; SULFONYLUREA DERIVATIVE;

ACIDIFICATION; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL LABELING; CELL STIMULATION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EXOCYTOSIS; FLUORESCENCE ANALYSIS; GENE FUSION; IMAGING SYSTEM; IMMUNOELECTRON MICROSCOPY; INSULIN RELEASE; INTRACELLULAR TRANSPORT; MOLECULAR MECHANICS; MOUSE; NONHUMAN; PANCREAS ISLET CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROCESSING; PROTEIN SECRETION; PROTEIN TARGETING; PROTEIN TRANSPORT; QUANTITATIVE ANALYSIS; RADIOIMMUNOASSAY; REGULATORY MECHANISM; REPORTER GENE; SECRETORY GRANULE; SECRETORY VESICLE; STIMULUS RESPONSE; ANIMAL; BAGG ALBINO MOUSE; C57BL MOUSE; DRUG EFFECT; IN VITRO STUDY; KINETICS; METABOLISM; PANCREAS ISLET; SECRETION; ULTRASTRUCTURE;

ANIMAL; C-PEPTIDE; EXOCYTOSIS; GLUCOSE; IN VITRO; INSULIN; ISLETS OF LANGERHANS; KINETICS; LUMINESCENT PROTEINS; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; MICROSCOPY, CONFOCAL; MICROSCOPY, IMMUNOELECTRON; RECOMBINANT FUSION PROTEINS; SECRETORY VESICLES; SULFONYLUREA COMPOUNDS; SUPPORT, NON-U.S. GOV'T; ANIMALS; GREEN FLUORESCENT PROTEINS;

EID: 0036017727     PISSN: 13989219     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0854.2002.30703.x     Document Type: Article

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