Probing protein-DNA interactions by unzipping a single DNA double helix

Biophysical Journal

Volumn 83, Issue 2, 2002, Pages 1098-1105

  Koch, Steven J ^a   Shundrovsky, Alia ^a   Jantzen, Benjamin C ^a   Wang, Michelle D ^a,b  

^a Department of Molecular Biology and Genetics   (United States)

^b 518 Clark Hall ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RESTRICTION ENDONUCLEASE; DNA;

ARTICLE; CALIBRATION; DNA HELIX; ENZYME ACTIVITY; EQUILIBRIUM CONSTANT; FEEDBACK SYSTEM; MOLECULAR DYNAMICS; PROTEIN DETERMINATION; PROTEIN DNA INTERACTION; BIOPHYSICS; CHEMISTRY; CONFORMATION; DNA DENATURATION; KINETICS; MECHANICAL STRESS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN BINDING; TIME;

BASE SEQUENCE; BIOPHYSICS; CALIBRATION; DNA; DNA RESTRICTION ENZYMES; KINETICS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; NUCLEIC ACID DENATURATION; PROTEIN BINDING; STRESS, MECHANICAL; TIME FACTORS;

EID: 0035997065     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(02)75233-8     Document Type: Article

References (25)

1. Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers
2. Processive translocation and DNA unwinding by individual RecBCD enzyme molecules
3. Molecular stick-slip motion revealed by opening DNA with piconewton forces
4. DNA strand separation studied by single molecule force measurements
5. Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA
6. High resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping
7. Pulling a single chromatin fiber reveals the forces that maintain its higher-order structure
8. Chi-sequence recognition and DNA translocation by single RecBCD helicase/nuclease molecules
9. Mechanical separation of the complementary strands of DNA
10. Role of the hydrophobic effect in stability of site-specific protein-DNA complexes
11. Coordinate ion pair formation between EcoRI endonuclease and DNA
12. Whole-genome shotgun optical mapping of Deinococcus radiodurans
13. Catalytic activity of the yeast SWI/ SNF complex on reconstituted nucleosome arrays
14. Stretching DNA
15. Cloning and sequence comparison of Aval and BsoBI restriction modification systems
16. Transcription by single molecules of RNA polymerase observed by light microscopy
17. Ordered restriction maps of Saccharomyces cerevisiae chromosomes constructed by optical mapping
18. Overstretching B-DNA: The elastic response of individual double-stranded and single-stranded DNA molecules
19. Single-molecule analysis of DNA uncoiling by a type II topoisomerase
20. Thermodynamic parameters governing interaction of EcoRI endonuclease with specific and nonspecific DNA sequences
21. Force and velocity measured for single molecules of RNA polymerase
22. Stretching DNA with optical tweezers
23. Single-molecule studies of the effect of template tension on T7 DNA polymerase activity
24. Transcription against an applied force
25. Restriction enzyme bsobi-DNA complex. A tunnel for recognition of degenerate DNA sequences and potential histidine catalysis