Anti-U1A monoclonal antibodies recognize unique epitope targets of U1A which are involved in the binding of U1 RNA

Journal of Molecular Recognition

Volumn 15, Issue 3, 2002, Pages 163-170

  Lutz, Carol S ^a,e   McClain, Micah T ^b,c   Harley, John B ^b,c,d   James, Judith A ^b,c  

^a RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

^b OKLAHOMA MEDICAL RESEARCH FOUNDATION   (United States)

^c UNIVERSITY OF OKLAHOMA HEALTH SCIENCES CENTER   (United States)

^d VETERANS AFFAIRS MEDICAL CENTER   (United States)

^e UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY   (United States)

Author keywords

Anti U1A; Epitope; Monoclonal antibodies; Spliceosome; U1A

Indexed keywords

AMINO ACID SEQUENCE; ANIMAL; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; BINDING SITES; BLOTTING, WESTERN; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; HELA CELLS; HUMAN; MICE; MICE, INBRED BALB C; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN CONFORMATION; RIBONUCLEOPROTEIN, U1 SMALL NUCLEAR; RNA, SMALL NUCLEAR; RNA-BINDING PROTEINS; SENSITIVITY AND SPECIFICITY; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.; SURFACE PLASMON RESONANCE; ANIMALS; HUMANS;

EUKARYOTA;

EPITOPE; MONOCLONAL ANTIBODY; RNA BINDING PROTEIN; SMALL NUCLEAR RIBONUCLEOPROTEIN; SMALL NUCLEAR RNA; U1 SMALL NUCLEAR RNA; U1A PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ANTIBODY SPECIFICITY; ARTICLE; BAGG ALBINO MOUSE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; DRUG ANTAGONISM; ENZYME LINKED IMMUNOSORBENT ASSAY; HELA CELL; HUMAN; IMMUNOLOGY; METABOLISM; MOUSE; PROTEIN BINDING; PROTEIN CONFORMATION; SENSITIVITY AND SPECIFICITY; SURFACE PLASMON RESONANCE; WESTERN BLOTTING;

EID: 0035994090     PISSN: 09523499     EISSN: None     Source Type: Journal    
DOI: 10.1002/jmr.569     Document Type: Article

References (49)

1. Solution structure of the N-terminal RNP domain of U1A protein: The role of the C-terminal residues in structure stability and RNA binding
2. The diverse world of small nuclear ribonucleoproteins
3. Conserved structures and diversity of functions of RNA-binding proteins
4. Splicing of precursors to mRNA by the spliceosomes
5. Monoclonal antibody characterization of the C proteins of heterogeneous nuclear ribonucleoprotein complexes in vertebrate cells
6. Antibodies to snRNPs in systemic lupus erythematosus
7. Pattern of anti-small nuclear ribonucleoprotein antibodies in MRL/Mp-Ipr/Ipr mice suggests that the intact U1 snRNP particle is their autoimmunogenic target
8. The human U1A snRNP protein regulates polyadenylation via a direct interaction with poly(A) polymerase
9. Structure of the RNA-dependent RNA polymerase of poliovirus
10. A model for disease heterogeneity in systemic lupus erythematosus
11. RNA-binding domain of the A protein component of the U1 small nuclear ribonucleoprotein analyzed by NMR spectroscopy is structurally similar to ribosomal proteins
12. NMR studies of U1 snRNA recognition by the N-terminal RNP domain of the human U1A protein
13. Human anti-Ro autoantibodies bind multiple conformational epitopes of 60-kD Ro autoantigen
14. Human lupus anti-spliceosome A protein autoantibodies bind contiguous surface structures and segregate into two sequential epitope binding patterns
15. Sequential autoantigenic determinants of the small nuclear ribonucleoprotein Sm D shared by human lupus autoantibodies and MRL Ipr/Ipr antibodies
16. Side-chain specificities and molecular modelling of peptide determinants for two anti-Sm B/B′ autoantibodies
17. Global and local dynamics of the human U1A protein determined by tryptophan fluorescence
18. Dictionary of protein secondary structure: Pattern recognition of hydrogen-bonded and geometrical features
19. Crystal structure of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs
20. RNA recognition: Towards determinants of specificity
21. MOLMOL: A program for display and analysis of macromolecular structures
22. RNA binding mediates the local cooperativity between the beta sheet and the C-terminal tail of the human U1A RBD1 protein
23. Visualization of P-selectin glycoprotein ligand-1 as a highly extended molecule and mapping of protein epitopes for monoclonal antibodies
24. An RBD that does not bind RNA: NMR secondary structure determination and biochemical properties of the C-terminal RNA binding domain from the human U1A protein
25. Tertiary structure of RBD2 and backbone dynamics of RBD1 and RBD2 of the human U1A protein determined by NMR spectroscopy
26. Structure of spliceosomal snRNPs and their role in pre-mRNA splicing
27. The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs
28. Quantitative determination that one of two potential RNA-binding domains of the A protein component of the U1 small nuclear ribonucleoprotein complex binds with high affinity to stemloop II of U1 RNA
29. Direct interaction of the U1snRNP-A protein with the upstream efficiency element of the SV40 late polyadenylation signal
30. Interaction between the U1snRNP-A protein and the 160-kD subunit of cleavage-polyadenylation specificity factor increases polyadenylation efficiency in vitro
31. The snRNP-free U1A (SF-A) complex(es): Identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor
32. Anti-Sm autoantibodies in systemic lupus erythematosus target highly basic surface structures of spliceosomal complexes
33. Splicing of precursors to messenger RNA by the spliceosome
34. Crystal structure of the RNA-binding domain of the U1 small nuclear ribonucleoprotein A
35. Identification of a novel, non-snRNP protein complex containing U1A protein
36. Crystal structure at 1.92A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin
37. Anti-DNA antibodies in systemic lupus erythematosus
38. Comparative analysis of epitope recognition of glutamic acid decarboxylase (GAD) by autoantibodies from different autoimmune disorders
39. Antibodies to ribonuclear proteins
40. Identification of the RNA binding segment of human U1A protein and definition of its binding site on U1 snRNA
41. High-resolution autoreactive epitope mapping and structural modeling of the 65 kDa form of human glutamic acid decarboxylase
42. Protein-protein interaction of the Ro-ribonucleoprotein particle using multiple antigenic peptides
43. cDNA cloning of the human U1 snRNA-associated A protein: Extensive homology between U1 and U2-specific proteins
44. Antinuclear antibodies: Diagnostic markers for autoimmune diseases and probes for cell biology
45. Characteristics of a soluble nuclear antigen precipitating with sera of patients with systemic lupus erythematosus
46. Protein functions in pre-mRNA splicing
47. Epitopic regions for antibodies against tumor necrosis factor alpha. Analysis by synthetic peptide mapping
48. The growing world of small nuclear ribonucleoproteins