Production of the baculovirus-expressed dengue virus glycoprotein NS1 can be improved dramatically with optimised regimes for fed-batch cultures and the addition of the insect moulting hormone, 20-Hydroxyecdysone

Journal of Virological Methods

Volumn 105, Issue 1, 2002, Pages 87-98

  Chan, Leslie C L ^a   Young, Paul R ^a   Bletchly, Cheryl ^a   Reid, Steven ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

20 Hydroxyecdysone; Baculovirus expression vector system; Dengue NS1 glycoprotein; Fed batch cultures; High yield

Indexed keywords

BETA GALACTOSIDASE; DENGUE VIRUS NON STRUCTURAL GLYCOPROTEIN 1; ECDYSTERONE; INSECT HORMONE; UNCLASSIFIED DRUG; VIRUS GLYCOPROTEIN; NS1 PROTEIN, DENGUE VIRUS TYPE 2; RECOMBINANT PROTEIN; VIRUS PROTEIN;

ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CELL DENSITY; CONTROLLED STUDY; CULTURE OPTIMIZATION; DENGUE VIRUS; FED BATCH CULTURE; GLYCOPROTEIN SYNTHESIS; GLYCOSYLATION; NONHUMAN; PRIORITY JOURNAL; ANIMAL; ARMYWORM; CELL LINE; CULTURE MEDIUM; EVALUATION; GENE VECTOR; GENETICS; METABOLISM; POLYHEDROSIS VIRUS;

ANIMAL; BETA-GALACTOSIDASE; CELL LINE; CULTURE MEDIA; DENGUE VIRUS; ECDYSTERONE; GENETIC VECTORS; NUCLEOPOLYHEDROVIRUS; RECOMBINANT PROTEINS; SPODOPTERA; VIRAL NONSTRUCTURAL PROTEINS; ANIMALS;

DENGUE VIRUS; INSECTA; UNIDENTIFIED BACULOVIRUS; VECTORS;

EID: 0035993309     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(02)00084-8     Document Type: Article

References (32)

1. Growth, nutrient consumption, and end-product accumulation in Sf-9 and BTI-EAA insect cell cultures: Insights into growth limitation and metabolism
2. 7 cells/ml and improves baculovirus-expressed recombinant protein yields
3. Cytogenetic analysis of ecdysteroid action
4. A perfusion process for the high density insect cell cultures
5. In vitro production studies wish a wild-type Helicoverpa baculovirus
6. Optimising fed-batch production of recombinant proteins using the baculovirus expression vector system
7. Physical factors that affect in vitro Autographa californica nuclear polyhedrosis vims infection
8. 7 cells/ml and production of β-Galactosidase at high cell density by fed batch culture
9. Immunoaffinity purification of native dimer forms of the flavivirus non-structural glycoprotein, NS1
10. Production of dimer-specific and dengue virus group cross-reactive mouse monoclonal antibodies to the dengue 2 virus non-structural glycoprotein NS1
11. The XXth century dengue pandemic: Need for surveillance and research
12. Influence of different signal peptides and prosequences on expression and secretion of human tissue plasminogen activator in the baculovirus system
13. Baculovirus expression vectors
14. Dengue: A continuing challenge for molecular biology
15. Maturation of the dengue-2 virus NS1 protein in insect cells: Effects of downstream NS2A sequences on baculovirus-expressed gene constructs
16. Isolation and identification of 20-hydroxyecdysone from a Lepidopteran continuous cell line
17. Effect of ammonium ion and extracellular pH on hybridoma cell metabolism and antibody production
18. Fed-batch culture of insect cells: A method to increase the yield of recombinant human nerve growth factor (rhNGF) in the baculovirus expression system
19. Hemocytometer cell count distributions: Implications of non-poisson behaviour
20. A baculovirus blocks insect molting by producing ecdysteroid UDP-glucosyltransferase
21. Modelling and optimization of the baculovirus expression system in batch suspension culture
22. Production of recombinant proteins in high cell-density insect cell cultures
23. Structure of the flavivirus genome
24. Ecdysteroids increase the yield of recombinant protein produced in baculovirus insect cell expression system
25. Protection of mice against dengue-2 virus enchephalitis by immunization with the dengue-2 virus nonstructural glycoprotein NS-1
26. Comparison of mammalian and insect cell cultures
27. Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system
28. Relationship between oxygen uptake rate and time of infection of Sf9 cells infected with a recombinant baculovirus
29. Low multiplicity infection of insect cells with a recombinant baculovirus: The cell yield concept
30. Rational scale-up of a baculovirus-insect cell batch process based on medium nutritional depth
31. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients
32. Optimization of the physiochemical parameters for the culture of Bombyx mori insect cells used in recombinant protein production