Autologous lymphocyte-monocyte co-culture increases NMR-visible and cytoplasmic lipids in the absence of increased markers of lymphocyte activation

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

Volumn 1533, Issue 3, 2001, Pages 243-254

  Delikatny, Edward J ^a,b   Van Holst Pellekaan, Caitlin J ^a   King, Nicholas J C ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

^b HOSPITAL OF THE UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

1H Nuclear magnetic resonance; Lipid accumulations; Lymphocyte activation; Lymphocyte monocyte interaction; Neutral lipid; NMR visible lipid

Indexed keywords

ANTIBODY; CD69 ANTIGEN; CD71 ANTIGEN; FAT DROPLET; INTERLEUKIN 2 RECEPTOR; IONOMYCIN; LIPID; PHORBOL 13 ACETATE 12 MYRISTATE; T LYMPHOCYTE RECEPTOR;

ANTIGEN EXPRESSION; ARTICLE; BLOOD CULTURE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; HUMAN; LYMPHOCYTE PROLIFERATION; MONOCYTE; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; T LYMPHOCYTE ACTIVATION;

ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, B-LYMPHOCYTE; ANTIGENS, DIFFERENTIATION, T-LYMPHOCYTE; AZO COMPOUNDS; COCULTURE TECHNIQUES; FLOW CYTOMETRY; HEMATOXYLIN; HUMANS; IONOMYCIN; LIPIDS; LYMPHOCYTE ACTIVATION; LYMPHOCYTES; MAGNETIC RESONANCE SPECTROSCOPY; MONOCYTES; PHOTOMICROGRAPHY; RECEPTORS, INTERLEUKIN-2; RECEPTORS, TRANSFERRIN; STAINING AND LABELING; TETRADECANOYLPHORBOL ACETATE;

EID: 0035980730     PISSN: 13881981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1388-1981(01)00157-3     Document Type: Article

References (29)

1. Magnetic resonance study of lymphocytes stimulated with concanavalin A
2. 1H MR spectrum in murine T-cells and B-cells occurs in G1 phase of cell cycle
3. An NMR investigation of the changes in plasma membrane triglyceride and phospholipid precursors during the activation of T-lymphocytes
4. 1H NMR studies of membrane changes during the activation of primary T-lymphocytes
5. 1H-NMR visible neutral lipids in activated T lymphocytes: Relationship to phosphatidylcholine cycling
6. Magnetic resonance studies of murine macrophages: Proliferation is not a prerequisite for acquisition of an 'activated' high resolution spectrum
7. 1H Magnetic resonance spectroscopy of primary human and murine cells of the myeloid lineage
8. 1H MR-visible lipid in human glioma cells is independent of the cell cycle
9. NMR methods for characterising the state of the surfaces of complex mammalian cells
10. 1H-NMR-detectable mobile lipid in stimulated lymphocytes: Relationship to cellular activation, the cell cycle, and phosphatidyl-choline specific phospholipase C
11. Assignment of methylene proton resonances in NMR spectra of embryonic and transformed cells to plasma membrane triglyceride
12. Identification of triglyceride in malignant cell membranes
13. Effect of viscosity on the width of the methylene proton magnetic resonance line in sonicated bilayer vesicles
14. 31P-NMR lineshapes
15. 1H NMR spectra of a myeloma cell line correlates with the induced formation of cytoplasmic lipid droplets
16. The relationship between nuclear magnetic resonance-visible lipids, lipid droplets, and cell proliferation in cultured C6 cells
17. Evidence that mobile lipids detected in rat brain glioma by H1 nuclear magnetic resonance correspond to lipid droplets
18. 1H MR spectra of neutrophils
19. 1H magnetic resonance signals from malignant cells do not arise from intracellular lipid droplets
20. 1H-NMR-detectable mobile lipid domains in NIH-3T3 fibroblasts
21. Lipid signals detected by NMR proton spectroscopy of whole cells are not correlated to lipid droplets evidenced by the nile red staining
22. 1H NMR-visible triacylglycerol in human neutrophils: High fatty acid ennvironments result in preferential sequestration of palmitic acid into plasma membrane triacylglycerol
23. Tetraphenylphosphonium chloride induced MR-visible lipid accumulation in a malignant human breast cell line
24. Modulation of MR-visible mobile lipid levels by cell culture conditions and correlations with chemotactic response
25. Induction of magnetic resonance-visible lipid in a transformed human breast cell line by tetraphenylphosphonium chloride
26. Biosynthesis of triacylglycerols
27. Lipoproteins may provide fatty acids necessary for human lymphocyte proliferation by both low density lipoprotein receptor-dependent and -independent mechanisms
28. Mitogenic stimulation alters the regulation of LDL receptor gene expression in human lymphocytes