Molecular cloning of kpcA gene encoding a Kex2p-like endoprotease from Aspergillus nidulans

Molecules and Cells

Volumn 12, Issue 1, 2001, Pages 142-147

  Kwon, Bong Kyu ^a   Han, Kap Hoon ^a   Han, Kyu Yong ^a   Ju, Sung Min ^a   Hwang, Sang Gu ^b   Jeon, Byung Hun ^b   Han, Dong Min ^a   Kim, Won Sin ^a  

^a Wonkwang University   (South Korea)

^b Wonkwang University   (South Korea)

Author keywords

KexB; Kexin; Proprotein Convertase; Proprotein Processing; Subtilisin Serine Protease

Indexed keywords

FUNGAL PROTEIN; KEX2 PROTEIN, S CEREVISIAE; PROTEINASE; SACCHAROMYCES CEREVISIAE PROTEIN; SERINE PROTEINASE; SUBTILISIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; ASPERGILLUS NIDULANS; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; HUMAN; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; SEQUENCE ALIGNMENT;

AMINO ACID SEQUENCE; ANIMALS; ASPERGILLUS NIDULANS; BASE SEQUENCE; CLONING, MOLECULAR; ENDOPEPTIDASES; FUNGAL PROTEINS; HUMANS; MOLECULAR SEQUENCE DATA; PROPROTEIN CONVERTASES; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; SUBTILISINS;

EID: 0035980115     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Anderson, E. D., Van Slyke, J. K., Thulin, C. D., Jean, F., and Thomas, G. (1997) Activation of the furin endoprotease is a multiple-step process: requirements for acidification and internal propeptide cleavage. EMBO J. 16, 1508-1518.
2. Andrews, P. C., Brayton, K., and Dixon, J. E. (1987) Related Articles Precursors to regulatory peptides: their proteolytic processing. Experientia 43, 784-790.
3. Archer, D. B., Jeenes, D. J., and Mackenzie, D. A. (1994) Strategies for improving heterologous protein production from filamentous fungi. Antonie Van Leeuwenhoek. 65, 245-250.
4. Basak, A., Schmidt, C., Ismail, A. A., Seidah, N. G., Chretien, M., and Lazure, C. (1995) Peptidyl substrates containing unnatural amino acid at the P'l position are potent inhibitors of prohormone convertases. Int. J. Pept. Protein Res. 46, 228-237.
5. Brenner, C. and Fuller, R. S. (1992) Structural and enzymatic characterization of a purified prohormone-proccssing enzyme: secreted, soluble KEX2 protease. Proc. Natl. Acad. Sci. USA 89, 922-926.
6. Broekhuijsen, M. P., Mattern, I. E., Contreras, R., Kinghorn, J. R., and van den Hondel, C. A. (1993) Secretion of heterologous proteins by Aspergillus niger: production of active human interleukin-6 in a protease-deficient mutant by KEX2-like processing of a glucoamylase-hIL6 fusion protein. J. Biotechnol. 31, 135-145.
7. Cho, K., Won, D. H., Cha, G., and Lee, C. C. (2000) Regulation of Mst57Dc expression in male accessory glands of Drosophila melanogaster. Mol. Cells 10, 180-185.
8. Contreras, R., Carrez, D., Kinghorn, J. R., van den Hondel, C. A., and Fiers, W. (1991) Efficient KEX2-like processing of a glucoamylase-interleukin-6 fusion protein by Aspergillus nidulans and secretion of mature interleukin-6. Biotechnology (NY) 9, 378-381.
9. Creemers, J. W., Siezen, R. J., Roebroek, A. J., Ayoubi, T. A., Huylebroeck, D., and Van de Ven, W. J. (1993) Modulation of furin-mediated proprotein processing activ ty by site-directed mutagenesis. J. Biol. Chem. 268, 21826-21834.
10. Creemers, J. W., Vey, M., Schafer, W., Ayoubi, T. A., Roebroek, A. J., Klenk, H. D., Garten, W., and Van de Yen, W. J. (1995) Endoproteolytic cleavage of its propeptide is a prerequisite for efficient transport of furin out of the endoplasmic reticulum. J. Biol. Chem. 270, 2695-2702.
11. Davey, J., Davis, K., Imai, Y., Yamamoto, M., and Matthews, G. (1994) Isolation and characterization of krp, a dibasic endopeptidase required for cell viability in the fission yeast Schizoxaccharomyces pombe. EMBO J. 13, 5910-5921.
12. Enderlin, C. S. and Ogrydziak, D. M. (1994) Cloning, nucleotide sequence and functions of XPR6, which codes for a dibasic processing endoprotease from the yeast Yarrowia lipolytica. Yeast 10, 67-79.
13. Gouka, R. J., Punt, P. J., and van den Hondel, C. A. (1997) Efficient production of secreted proteins by Aspergillus: progress, limitations and prospects. Appl. Microbiol. Biotechnol. 47, 1-11.
14. Hatsuzawa, K., Nagahama, M., Takahashi, S., Takada, K., Murakami, K., and Nakayama, K. (1992) Purification and characterization of furin, a Kex2-like processing endoprotease, produced in Chinese hamster ovary cells. J. Biol. Chem. 267, 16094-16099.
15. Jalving, R., van de Vondervoort, P. J., Visser, J., and Schaap, P. J. (2000) Characterization of the kexin-like maturase of Aspergillus niger. Appl. Environ. Microbiol. 66, 363-368.
16. Leduc, R., Molloy, S. S., Thorne, B. A., and Thomas, G. (1992) Activation of human furin precursor processing endoprotease occurs by an intramolecular autoproteolytic cleavage. J. Biol. Chem. 267, 14304-14308.
17. Matthews, G., Shennan, K. I., Seal, A. J., Taylor, N. A., Colman, A., and Docherty, K. (1994) Autocatalytic maturation of the prohormone convertase PC2. J. Biol. Chem. 269, 588-592.
18. Mikosch, T., Klemm, P., Gassen, H. G., van den Hondel, C. A., and Kemme, M. (1996) Secretion of active human mucus proteinase inhibitor by Aspergillus niger after KEX2-like processing of a glucoamylase-inhibitor fusion protein. J. Biotechnol. 52, 97-106.
19. Misumi, Y., Oda, K., Fujiwara, T., Takami, N., Tashiro, K., and Ikehara, Y. (1991) Functional expression of furin demonstrating its intracellular localization and endoprotease activity for processing of proalbumin and complement pro-C3. J. Biol. Chem. 266, 16954-16959.
20. Nakayama, K. (1997) Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins. Biochem. J. 327, 625-635.
21. Newport, G. and Agabian, N. (1997) KEX2 influences candida albicans proteinase secretion and hyphal formation. J. Biol. Chem. 272, 28954-28961.
22. Powner, D. and Davey, J. (1997) Autocatalytic removal of the pro-sequence from kexins requires an intact P-domain. Biochem. Soc. Trans. 25, S626.
23. Rouille, Y., Duguay, S. J., Lund, K., Furuta, M., Gong, Q., Lipkind, G., Oliva, A. A., Chan, S. J., and Steiner, D. F. (1995) Proteolytic processing mechanisms in the biosynthesis of neuroendocrine peptides: the subtilisin-like proprotein convertases. Front. Neuroendocrinol. 16, 322-361.
24. Seidah, N. G., Galanopoulou, A. S., and Patel, Y. C. (1995) Heterologous processing of rat prosomatostatin to somatostatin-14 by PC2: requirement for secretory cell but not the secretion granule. Biochem. J. 311, 111-118.
25. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C. (1985) Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76-78.
26. Steiner, D. F., Smeekens, S. P., Ohagi, S., and Chan, S. J. (1992) The new enzymology of precursor processing endoproteases. J. Biol. Chem. 267, 23435-23438.
27. Tanguy-Rougeau, J. and Yeh, J. Z. (1988) Batrachotoxin uncouples gating charge immobilization from fast Na inactivation in squid giant axons. Biophys. J. 54, 719-730.
28. Thomas, G., Thorne, B. A., Thomas, L., Allen, R. G., Hruby, D. E., Fuller, R., and Thorner, J. (1988) Yeast KEX2 endopeptidase correctly cleaves a neuroendocrine prohormone in mammalian cells. Science 241, 226-230.
29. Vey, M., Schafer, W., Berghofer, S., Klenk, H. D., and Garten, W. (1994) Maturation of the trans-Golgi network protease furin: compartmentalization of propeptide removal, substrate cleavage, and COOH-terminal truncation. J. Cell. Biol. 127, 1829-1842.
30. Yu, J.-H., Butchko, R. A., Fernandes, M., Keller, N. P., Leonard, T. J., and Adams, T. H. (1996) Conservation of structure and function of the aflatoxin regulatory gene aflR from Aspergillus nidulans and A. Flavus. Curr. Genet. 29, 549-555.