Simplified serum- and steroid-free culture conditions for high-throughput viability analysis of primary cultures of cerebellar granule neurons

Journal of Neuroscience Methods

Volumn 110, Issue 1-2, 2001, Pages 45-55

  Wong, Jeremy K ^a   Kennedy, Patrick R ^a   Belcher, Scott M ^a  

^a UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

Author keywords

Cell culture; Cerebellum; Excitotoxicity; Glutamate; LDH release; MK 801; MTT; Neuron; Neuroprotection; Neurotoxicity; Reactive oxygen

Indexed keywords

3 (4,5 DIMETHYLTHIAZOL 2 YL) 5 (3 CARBOXYMETHOXYPHENYL) 2 (4 SULFOPHENYL) 2H TETRAZOLIUM; DIZOCILPINE; EXCITOTOXIN; GLUTAMATE RECEPTOR; LACTATE DEHYDROGENASE; N METHYL DEXTRO ASPARTIC ACID RECEPTOR BLOCKING AGENT; TETRAZOLIUM; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; ASSAY; AUTOANALYSIS; CELL DEATH; CELL DENSITY; CELL VIABILITY; CEREBELLUM; CONTROLLED STUDY; DENSITOMETRY; FEMALE; GRANULE CELL; MALE; NERVE CELL; NEUROPROTECTION; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; RAT; TISSUE CULTURE;

ANIMALS; ANIMALS, NEWBORN; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CELLS, CULTURED; CEREBELLAR CORTEX; COLORING AGENTS; CULTURE MEDIA, SERUM-FREE; DOSE-RESPONSE RELATIONSHIP, DRUG; EXCITATORY AMINO ACID ANTAGONISTS; FEMALE; GLUTAMIC ACID; L-LACTATE DEHYDROGENASE; MALE; NEURONS; NEUROPHYSIOLOGY; NEUROPROTECTIVE AGENTS; OXIDATIVE STRESS; RATS; RATS, SPRAGUE-DAWLEY; STEROIDS; TETRAZOLIUM SALTS; THIAZOLES;

EID: 0035975671     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(01)00419-8     Document Type: Article

References (27)

1. Properties of neurons from dissociated fetal rat brain in serum-free culture
2. Brain-derived neurotrophic factor mediates the anti-apoptotic effect of NMDA in cerebellar granule neurons: Signal transduction cascades and site of ethanol action
3. Growth of a rat neuroblastoma cell line in serum-free supplemented medium
4. Selective survival of neurons from chick embryo sensory ganglionic dissociates utilizing serum-free supplemented medium
5. Serum-free B27/neurobasal medium supports differentiated growth of neurons from the striatum, substantia nigra, septum, cerebral cortex, cerebellum, and dentate gyrus
6. Survival and growth of hippocampal neurons in defined medium at low density: Advantages of a sandwich culture technique or low oxygen
7. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination
8. Neurotrophic effects of NMDA receptor activation on developing cerebellar granule cells
9. Investigations of non-NMDA receptor-induced toxicity in serum-free antioxidant-rich primary cultures of murine cerebellar granule cells
10. Zinc and brain injury
11. Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture
12. Noise and single channels activated by excitatory amino acids in rat cerebellar granule neurones
13. Hormonal regulation of the mRNA encoding the ketogenic enzyme mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase in neonatal primary cultures of cortical astrocytes and meningeal fibroblasts
14. Rapid action of 17β-estradiol on kainate-induced currents in hippocampal neurons lacking intracellular estrogen receptors
15. 17 beta-Estradiol potentiates kainate-induced currents via activation of the cAMP cascade
16. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill
17. Responses to N-methyl-d-aspartate and kainic acid in cerebellar granule cells of lead-exposed rat pups
18. Cytotoxic amyloid peptides inhibit cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction by enhancing MTT formazan exocytosis
19. Mechanism of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction
20. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
21. High-affinity kainate-type ion channels in rat cerebellar granule cells
22. The mitogen-activated protein kinase pathway mediates estrogen neuroprotection after glutamate toxicity in primary cortical neurons
23. Estrogen-induced activation of mitogen-activated protein kinase in cerebral cortical explants: Convergence of estrogen and neurotrophin signaling pathways
24. Estrogen administration increases neuronal responses to excitatory amino acids as a long-term effect
25. Progesterone enhances inhibitory responses of cerebellar Purkinje cells mediated by the GABAA receptor subtype
26. Progesterone administration attenuates excitatory amino acid responses of cerebellar Purkinje cells
27. Novel mechanisms of estrogen action in the brain: New players in an old story