Solution-state NMR investigations of triosephosphate isomerase active site loop motion: Ligand release in relation to active site loop dynamics

Journal of Molecular Biology

Volumn 310, Issue 1, 2001, Pages 271-280

  Rozovsky, Sharon ^a   Jogl, Gerwald ^b   Tong, Liang ^b   McDermott, Ann E ^a  

^a Department of Chemistry ^*  (United States)

^b Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

5 fluorotryptophan; Chemical exchange; Flexible loop; Protein dynamics; Triosephosphate isomerase

Indexed keywords

DIHYDROXYACETONE PHOSPHATE; FLUORINE; GLYCERALDEHYDE 3 PHOSPHATE; GLYCEROPHOSPHATE; LIGAND; PROTEIN; TRIOSEPHOSPHATE ISOMERASE; WATER;

AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CRYSTALLOGRAPHY; ENZYME STRUCTURE; HYDROGEN BOND; ISOMERISM; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; REPORTER GENE;

EID: 0035967856     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4673     Document Type: Article

References (44)

1. On the three-dimensional structure and catalytic mechanism of triose phosphate isomerase
2. Free-energy profile of the reaction catalyzed by triosephosphate isomerase
3. Importance of tryptophan dipoles for protein function: 5-fluorination of tryptophans in gramicidin A channels
4. Critical ionization states in the reaction catalyzed by triosephosphate isomerase
5. Studies of triosephosphate isomerase by hydrogen exchange
6. Studies of histidine residues of triosephosphate isomerase by proton magnetic resonance and X-ray crystallography
7. Crystallography & NMR system: A new software suite for macromolecular structure determination
8. Enzyme-substrate and enzyme-inhibitor complexes of triose phosphate isomerase studied by 31P nuclear magnetic resonance
9. Structure of the triosephosphate isomerase-phosphoglycolo-hydroxamate complex: An analogue of the intermediate on the reaction pathway
10. Chemical modification of proteins by methylglyoxal
11. Fluorine NMR of proteins
12. Solvent-induced deuterium-isotope effects on F-19 chemical- shifts of some substituted fluorobenzenes: Formation of inclusion complexes
13. NMR studies of the role of hydrogen bonding in the mechanism of triosephosphate isomerase
14. a 6.0 in triosephosphate isomerase
15. a of the active-site carboxyl group
16. How good is fluorine as a hydrogen bond acceptor?
17. Spectrophotometric studies on the interaction between triose phosphate isomerase and inhibitors
18. Improved methods for building protein models in electron- density maps and the location of errors in these models
19. Enzyme catalysis: Not different, just better
20. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
21. Kinetic-properties of triose-phosphate isomerase From trypanosoma-brucei-brucei - A comparison with the rabbit muscle and yeast enzymes
22. Neutral imidazole is the electrophile in the reaction catalyzed by triosephosphate isomerase: Structural origins and catalytic implications
23. Crystallographic analysis of the complex between triosephosphate isomerase and 2-phosphoglycolate at 2.5 Å resolution: Implications for catalysis
24. Structure of yeast triosephosphate isomerase at 1.9 Å resolution
25. A TROSY CPMG sequence for characterizing chemical exchange in large proteins
26. Raster3D: Photo-realistic molecular graphics
27. Atomic mutations at the single tryptophan residue of human recombinant annexin V: Effects on structure, stability, and activity
28. Triosephosphate isomerase: Energetics of the reaction catalyzed by the yeast enzyme expressed in Escherichia coli
29. Processing of X-ray diffraction data collected in oscillation mode
30. Enzymes harboring unnatural amino acids: Mechanistic and structural analysis of the enhanced catalytic activity of a glutathione transferase containing 5-fluorotryptophan
31. pH-dependence of the triose phosphate isomerase reaction
32. Stabilization of a reaction intermediate as a catalytic device: Definition of the functional role of the flexible loop in triosephosphate isomerase
33. Specificity and kinetics of triose phosphate isomerase from chicken muscle
34. Kinetic parameters for the elimination reaction catalyzed by triosephosphate isomerase and an estimation of the reaction's physiological significance
35. Proton diffusion in the active-site of triosephosphate isomerase
36. The timescale of the catalytic loop motion in triosephosphate isomerase
37. Segmental motion in catalysis: Investigation of a hydrogen bond critical for loop closure in the reaction of triosephosphate isomerase
38. Segmental movement: Definition of the structural requirements for loop closure in catalysis by triosephosphate isomerase
39. Triosephosphate isomerase deficiency: Historical perspectives and molecular aspects
40. Active conformation of an inactive semi-synthetic ribonuclease-s
41. Phosphorus-31 nuclear magnetic resonance of dihydroxyacetone phosphate in the presence of triosephosphate isomerase. The question of non-productive binding of the substrate hydrate
42. Dynamics of the flexible loop of triosephosphate isomerase: The loop motion is not ligand gated
43. The hinged lid of yeast triose-phosphate isomerase. Determination of the energy barrier between the two conformations