Identification of the Enzymatic Active Site of Tobacco Caffeoyl-coenzyme A O-Methyltransferase by Site-directed Mutagenesis

Journal of Biological Chemistry

Volumn 276, Issue 39, 2001, Pages 36831-36838

  Hoffmann, Laurent ^a   Maury, Stéphane ^a,c   Bergdoll, Marc ^a   Thion, Laurent ^b   Erard, Monique ^b   Legrand, Michel ^a  

^a INST DE BIOL MOLEC DES PLANTES   (France)

^b INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

^c UNIVERSITÉ D'ORLÉANS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTALLOGRAPHY; ESCHERICHIA COLI; MUTAGENESIS; SUBSTRATES;

CIRCULAR DICHROISM;

COENZYMES;

ALANINE; ARGININE; ASPARTIC ACID; CAFFEOYL COENZYME A O METHYLTRANSFERASE; COENZYME A; GLUTAMINE; METHYLTRANSFERASE; SERINE; THREONINE; UNCLASSIFIED DRUG; ADENINE; CAFFEOYL COA O METHYLTRANSFERASE; CAFFEOYL-COA O-METHYLTRANSFERASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CIRCULAR DICHROISM; CONTROLLED STUDY; CRYSTALLOGRAPHY; DELETION MUTANT; ELECTRICITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; TOBACCO; ANIMAL; BINDING SITE; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; IMMUNOLOGY; KINETICS; LIVER; METABOLISM; MOLECULAR GENETICS; MUTATION; PLANT; PLASMID; PROTEIN BINDING; RAT; SWINE;

ANIMALIA; ESCHERICHIA COLI; NICOTIANA TABACUM; PUFFINUS AURICULARIS;

ADENINE; ALLERGY AND IMMUNOLOGY; AMINO ACID SEQUENCE; ANIMALS; ARGININE; BINDING SITES; CIRCULAR DICHROISM; ESCHERICHIA COLI; GLUTAMINE; KINETICS; LIVER; METHYLTRANSFERASES; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLANTS, TOXIC; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN BINDING; RATS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; SWINE; THREONINE; TOBACCO;

EID: 0035965202     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M104977200     Document Type: Article

References (40)

1. Dixon, R. A., and Paiva, N. L. (1995) Plant Cell 7, 1085-1097
2. Douglas, C. J. (1996) Trends Plant. Sci. 1, 171-178
3. Baucher, M., Monties, B., Van Montagu, M., and Boerjan, W. (1998) Crit. Rev. Plant Sci. 17, 125-197
4. Boudet, A. M., Lapierre, C., and Grima-Pettenati, J. (1995) New Phytol. 129, 203-236
5. Burns, J., Gardner, P., O'Neil, J., Crawford, S., Morecroft, I., McPhail, D., Lister, C., Matthews, D., MacLean, M., Lean, M., Duthie, G., and Crozier, A. (2000) J. Agric. Food Chem. 48, 220-230
6. Jang, M., Cai, L., Udeani, G., Slowing, K., Thomas, C., Beecher, C., Fong, H., Farnsworth, N., Kinghorn, A., Mehta, R., Moon, R., and Pezzuto, J. (1997) Science 275, 218-220
7. Kahkonen, M., Hopia, A., Vuorela, H., Rauha, J., Pihlaja, K., Kujala, T., and Heinonen, M. (1999) J. Agric. Food Chem. 47, 3954-3962
8. Bugos, R. C., Chiang, V. L., and Campbell, W. H. (1991) Plant Mol. Biol. 17, 1203-1215
9. Ibrahim, R. K., Bruneau, A., and Bantignies, B. (1998) Plant Mol. Biol. 36, 1-10
10. Jaeck, E., Dumas, B., Geoffroy, P., Favet, N., Inze, D., Van Montagu, M., Fritig, B., and Legrand, M. (1992) Mol. Plant-Microbe Interact. 5, 294-300
11. Joshi, C. P., and Chiang, V. L. (1998) Plant Mol. Biol. 37, 663-674
12. Pellegrini, L. O. G., Geoffroy, P., Fritig, B., and Legrand, M. (1993) Plant Physiol. 103, 509-517
13. Schmitt, D., Pakush, A.-E., and Matern, U. (1991) J. Biol. Chem. 266, 17416-17423
14. Ye, Z.-H., Kneusel, R. E., Matern, U., and Varner, J. E. (1994) Plant Cell 6, 1427-1439
15. Chen, C., Meyermans, H., Burggraeve, B., De Rycke, R., Inoue, K., De Vlesschauwer, V., Steenackers, M., Van Montagu, M., Engler, G., and Boerjan, W. (2000) Plant Physiol. 123, 853-867
16. Inoue, K., Sewalt, V. J. H., Ballance, G. M., Ni, W., Stürtzer, C., and Dixon, R. A. (1998) Plant Physiol. 117, 761-770
17. Martz, F., Maury, S., Pinçon, G., and Legrand, M. (1998) Plant Mol. Biol. 36, 427-437
18. Maury, S., Geoffroy, P., and Legrand, M. (1999) Plant Physiol. 121, 215-224
19. Zubieta, C., He, X.-Z., Dixon, R. A., and Noel, J. P. (2001) Nat. Struct. Biol. 8, 271-279
20. Assicot, M., and Bohuon, C. (1970) Eur. J. Biochem. 12, 490-495
21. Axelrod, J., and Tomchick, R. (1958) J. Biol. Chem. 233, 702-705
22. Bonifati, V., and Meco, G. (1999) Pharmacol. Ther. 81, 1-36
23. Kaakkola, S. (2000) Drugs 59, 1223-1250
24. Ruottinen, H., and Rinne, U. (1998) J. Neurol. 245, 25-34
25. Berman, H., Westbrook, J., Feng, Z., Gilliland, G., Bhat, T., Weissig, H., Shindyalov, I., and Bourne, P. (2000) Nucleic Acids Res. 28, 235-242
26. Vidgren, J., Svensson, L. A., and Liljas, A. (1994) Nature 368, 354-357
27. Bugl, H., Fauman, E., Stacker, B., Zheng, F., Kushner, S., Saper, M., Bardwell, J., and Jakob, U. (2000) Mol. Cell 6, 349-360
28. Schluckebier, G., Kozak, M., Bleimling, N., Weinhold, E., and Saenger, W. (1997) J. Mol. Biol. 265, 56-67
29. Skinner, M., Puvathingal, J., Wajter, R., and Friedman, A. (2000) Structure 8, 1189-1201
30. Zhang, X., Zhou, L., and Cheng, X. (2000) EMBO J. 19, 3509-3519
31. Sänger, F., Nicklen, S., and Coulson, A. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 5463-5467
32. Geoffroy, P., Legrand, M., and Fritig, B. (1990) Mol. Plant-Microbe Interact 3, 327-333
33. Stöckigt, J., and Zenk, M. H. (1975) Z. Naturforsch 30c, 352-358
34. Negrel, J., and Smith, T. A. (1984) Phytochemistry 23, 31-34
35. Lüderitz, T., Schatz, G., and Grisebach, H. (1982) Eur. J. Biochem. 123, 583-586
36. Sali, A., and Blundell, T. L. (1993) J. Mol. Biol. 234, 779-815
37. Jones, T. A., Bergdoll, M., and Kjeldgaard, M. (1989) in Crystallographic Computing and Modelling Methods in Macromolecular Design (Buggs, C., and Ealick, S., eds) pp. 189-195, Springer, NY
38. Collazo, P., Montoliu, L., Puigdomenech, P., and Rigau, J. (1992) Plant Mol. Biol. 20, 857-867
39. Legrand, M., Fritig, B., and Hirth, L. (1978) Planta 144, 101-108
40. Frick, S., and Kutcan, T. M. (1999) Plant J. 17, 329-339