Crystal structure of the D85S mutant of bacteriorhodopsin: Model of an O-like photocycle intermediate

Journal of Molecular Biology

Volumn 313, Issue 3, 2001, Pages 615-628

  Rouhani, Shahab ^a   Cartailler, Jean Philippe ^b   Facciotti, Marc T ^a   Walian, Peter ^a   Needleman, Richard ^c   Lanyi, Janos K ^d   Glaeser, Robert M ^a   Luecke, Hartmut ^b,d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Bacteriorhodopsin; Membrane protein; Photocycle intermediate; X ray crystallography

Indexed keywords

ARGININE; ASPARTIC ACID; BACTERIORHODOPSIN; GLYCEROL OLEATE; LIPID; SCHIFF BASE; TRYPTOPHAN;

ARTICLE; CIS TRANS ISOMERISM; CRYSTAL STRUCTURE; CRYSTALLIZATION; HYDRATION; HYDROGEN BOND; MOLECULAR MODEL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN STRUCTURE; PROTON TRANSPORT;

EID: 0035955561     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.5066     Document Type: Article

References (52)

1. Bacterial rhodopsin: Evidence for a new type of phototrophy in the sea
2. Lipidic cubic phases: A novel concept for the crystallization of membrane proteins
3. Structure of bacteriorhodopsin at 1.55 A resolution
4. Protein, lipid and water organization in bacteriorhodopsin crystals: A molecular view of the purple membrane at 1.9 Å resolution
5. Coupling photoisomerization of retinal to directional transport in bacteriorhodopsin
6. Structural changes in bacteriorhodopsin during ion transport at 2 angstrom resolution
7. Structural alterations for proton translocation in the M state of wild-type bacteriorhodopsin
8. High-resolution X-ray structure of an early intermediate in the bacteriorhodopsin photocycle
9. Helix deformation is coupled to vectorial proton transport in the photocycle of bacteriorhodopsin
10. Trapping and spectroscopic identification of the photointermediates of bacteriorhodopsin at low temperatures
11. Bacteriorhodopsin D85N: Three spectroscopic species in equilibrium
12. Time-resolved X-ray diffraction reveals multiple conformations in the M- N transition of the bacteriorhodopsin photocycle
13. Crystallographic analysis of protein conformational changes in the bacteriorhodopsin photocycle
14. Chromophore structure in bacteriorhodopsin's N intermediate: Implications for the proton-pumping mechanism
15. Partitioning of free energy gain between the photoisomerized retinal and the protein in bacteriorhodopsin
16. Relationship of retinal configuration and internal proton transfer at the end of the bacteriorhodopsin photocycle
17. Resonance Raman spectra of the "blue" and the regenerated "purple" membranes of Halobacterium halobium
18. Resonance Raman spectra of the acidified and deionized forms of bacteriorhodopsin
19. Interaction of proton and chloride transfer pathways in recombinant bacteriorhodopsin with chloride transport activity: Implications for the chloride translocation mechanism
20. Conversion of bacteriorhodopsin into a chloride ion pump
21. Bacteriorhodopsin: A light-driven proton pump in Halobacterium halobium
22. Determination of retinal chromophore structure in bacteriorhodopsin with resonance Raman spectroscopy
23. Structure of the bacteriorhodopsin mutant F219L N intermediate revealed by electron crystallography
24. A novel three-dimensional crystal of bacteriorhodopsin obtained by successive fusion of the vesicular assemblies
25. Lipid patches in membrane protein oligomers: Crystal structure of the bacteriorhodopsin-lipid complex
26. Orthorhombic two-dimensional crystal form of purple membrane
27. Structure of the light-driven chloride pump halorhodopsin at 1.8 Å resolution
28. Electrogenic processes and protein conformational changes accompanying the bacteriorhodopsin photocycle
29. Atomic resolution structures of bacteriorhodopsin photocycle intermediates: The role of discrete water molecules in the function of this light-driven ion pump
30. Chemical and physical evidence for multiple functional steps comprising the M state of the bacteriorhodopsin photocycle
31. Crystal structure of sensory rhodopsin II at 2.4 Å: Insights into color tuning and transducer interaction
32. The two pKa's of aspartate-85 and control of thermal isomerization and proton release in the arginine-82 to lysine mutant of bacteriorhodopsin
33. Titration of aspartate-85 in bacteriorhodopsin: What it says about chromophore isomerization and proton release
34. A linkage of the pKa's of asp-85 and glu-204 forms part of the reprotonation switch of bacteriorhodopsin
35. Evidence for the rate of the final step in the bacteriorhodopsin photocycle being controlled by the proton release group: R134H mutant
36. Bacteriorhodopsin's intramolecular proton-release pathway consists of a hydrogen-bonded network
37. Packing of aromatic rings against tryptophan residues in proteins
38. Kinetic and thermodynamic study of the bacteriorhodopsin photocycle over a wide pH range
39. Time-resolved Fourier transform infrared spectroscopy of the bacteriorhodopsin mutant Tyr185 → Phe: Asp96 reprotonates during O formation; Asp85 and Asp212 deprotonate during O decay
40. Local-access model for proton transfer in bacteriorhodopsin
41. C1-dependent photovoltage responses of bacteriorhodopsin: Comparison of the D85T and D85S mutants and wild-type acid purple form
42. Chloride and proton transport in bacteriorhodopsin mutant D85T: Different modes of ion translocation in a retinal protein
43. Proton transport by halorhodopsin
44. Reconciling crystallography and mutagenesis: A synthetic approach to the creation of a comprehensive model for proton pumping by bacteriorhodopsin
45. Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane
46. Processing of X-ray diffraction data collected in oscillation mode
47. Proton transfer pathways in bacteriorhodopsin at 2.3 angstrom resolution
48. Improved methods for the building of protein models in electron density maps and the location of errors in these models
49. Crystallography & NMR system: A new software suite for macromolecular structure determination
50. SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling