The Bacillus thuringiensis Cry1Ac toxin-induced permeability change in Manduca sexta midgut brush border membrane vesicles proceeds by more than one mechanism

Journal of Cell Science

Volumn 110, Issue 24, 1997, Pages 3099-3104

  Carroll, Joe ^a   Wolfersberger, Michael G ^b   Ellar, David J ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b Temple University   (United States)

Author keywords

Aminopeptidase N; Bacillus thuringiensis; Brush border membrane vesicle; Cry1Ac; Manduca sexta; Membrane; N acetylgalactosamine; Permeabilisation

Indexed keywords

BACILLUS THURINGIENSIS TOXIN; BINDING PROTEIN; MICROSOMAL AMINOPEPTIDASE; N ACETYLGALACTOSAMINE;

ARTICLE; BRUSH BORDER; CELL MEMBRANE PERMEABILITY; LEPIDOPTERA; MEMBRANE VESICLE; MIDGUT; PRIORITY JOURNAL;

ANIMALS; ANTIGENS, CD13; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; CELL MEMBRANE PERMEABILITY; ENDOTOXINS; HEMOLYSIN PROTEINS; MANDUCA; MICROVILLI; PROTEIN BINDING;

EID: 0031469739     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Belfiore, C. J., Vadlamudi, R. K., Osman, Y. A. and Bulla, L. A., Jr. (1994). A specific binding protein from Tenebrio molitor for the insecticidal toxin of Bacillus thuringiensis subsp. tenebrionis. Biochem. Biophys. Res. Comm. 200, 359-364.
2. Bell, R. A. and Joachim, F. G. (1976). Techniques for rearing laboratory colonies of tobacco hornworms and pink bollworms. Ann. Entomol. Soc. Am. 69, 365-373.
3. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
4. Bravo, A., Hendrickx, K., Jansens, S. and Peferoen, M. (1992a). Immunocytochemical analysis of specific binding of Bacillus thuringiensis insecticidal crystal proteins to lepidopteran and coleopteran midgut membranes. J. Invertebr. Pathol. 60, 247-253.
5. Bravo, A., Jansens, S. and Peferoen, M. (1992b). Immunocytochemical localization of Bacillus thuringiensis insecticidal crystal proteins in intoxicated insects. J. Invertebr. Pathol. 60, 237-246.
6. Carroll, J. and Ellar, D. J. (1993). An analysis of Bacillus thuringiensis δ-endotoxin action on insect-midgut-membrane permeability using a light-scattering assay. Eur. J. Biochem. 214, 771-778.
7. Carroll, J. and Ellar, D. J. (1997). Analysis of the large aqueous pores produced by a Bacillus thuringiensis protein insecticide in Manduca sexta midgut-brush-border-membrane vesicles. Eur. J. Biochem. 245, 797-804.
8. Dennis, R. D., Wiegandt, H., Haustein, D., Knowles, B. H. and Ellar, D. J. (1986). Thin layer chromatography overlay technique in the analysis of the binding of the solubilized protoxin of Bacillus thuringiensis var. kurstaki to an insect glycosphingolipid of known structure. Biomed. Chromatography 1, 31-37.
9. English, L., Robbins, H. L., Von Tersch, M. A., Kulesza, C. A., Ave, D., Coyle, D., Jany, C. S. and Slatin S. L. (1994). Mode of action of CryIIA: a Bacillus thuringiensis delta-endotoxin. Insect Biochem. Molec. Biol. 24, 1025-1035.
10. Feitelson, J. S., Payne J. and Kim L. (1992). Bacillus thuringiensis: Insects and beyond. Bio/Technology 10, 271-275.
11. Garczynski, S. F. and Adang M. J. (1996). Interactions of Bacillus thuringiensis CryI toxins with lipids isolated from the midgut of Manduca sexta larvae. In Abstracts of the 29th Society for Invertebrate Pathology Annual Meeting and IIIrd International Colloquium on Bacillus thuringiensis, p. 28. Society for Invertebrate Pathology, Cordoba, Spain.
12. Garczynski, S. F., Crim J. W. and Adang M. J. (1991). Identification of putative insect brush border membrane-binding molecules specific to Bacillus thuringiensis δ-endotoxin by protein blot analysis. Appl. Environ. Microbiol. 57, 2816-2820.
13. Gill, S. S., Cowles, E. A. and Francis V. (1995). Identification, isolation, and cloning of a Bacillus thuringiensis CryIAc toxin-binding protein from the midgut of the lepidopteran insect Heliothis virescens. J. Biol. Chem. 270, 27277-27282.
14. Grochulski, P., Masson, L., Borisova, S., Pusztai-Carey, M., Schwartz, J. L., Brousseau, R. and Cygler M. (1995). Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation. J. Mol. Biol. 254, 447-464.
15. Hafkensheid, J. C. M. (1984). Aminopeptidases and amino acid arylamidases. In Methods of Enzyme Analysis, Vol. V, Enzymes 3; Peptidases, Proteinases and their Inhibitors (ed. H. U. Bergmeyer), pp. 2-34. Verlag Chemie, Weinheim.
16. Hawkes, R., Niday, E. and Gordon, J. (1982). A dot-immunoblotting assay for monoclonal and other antibodies. Anal. Biochem. 119, 142-147.
17. Hofmann, C., Vanderbruggen, H., Höfte, H., Van Rie, J., Jansens, S. and Van Mellaert, H. (1988). Specificity of Bacillus thuringiensis δ-endotoxins is correlated with the presence of high-affinity binding sites in the brush border membrane of target insect midguts. Proc. Nat. Acad. Sci. USA 85, 7844-7848.
18. Höfte, H. and Whiteley, H. R. (1989). Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53, 242-255.
19. Knight, P. J. K., Crickmore, N. and Ellar, D. J. (1994). The receptor for Bacillus thuringiensis CryIA(c) delta-endotoxin in the brush border membrane of the lepidopteran Manduca sexta is aminopeptidase N. Mol. Microbiol. 11, 429-436.
20. Knowles, B. H. (1994). Mechanism of action of Bacillus thuringiensis δ-endotoxins. Adv. Insect Physiol. 24, 275-308.
21. Knowles, B. H. and Dow, J. A. T. (1993). The crystal δ-endotoxins of Bacillus thuringiensis: models for their mechanism of action on the insect gut. BioEssays 15, 469-476.
22. Knowles, B. H. and Ellar, D. J. (1987). Colloid-osmotic lysis is a general feature of the mechanism of action of Bacillus thuringiensis δ-endotoxins with different insect specificity. Biochim. Biophys. Acta 924, 509-518.
23. Knowles, B. H., Knight, P. J. K. and Ellar, D. J. (1991). N-acetyl galactosamine is part of the receptor in insect gut epithelia that recognizes an insecticidal protein from Bacillus thuringiensis. Proc. R. Soc. Land. B 245, 31-35.
24. Laemmli, U. K. and Favre, M. (1973). Maturation of the head of bacteriophage T4. I. DNA packaging events. J. Mol. Biol. 80, 575-599.
25. Lorence, A., Darszon, A., Díaz, C., Liévano, A., Quintero, R. and Bravo, A. (1995). δ-Endotoxins induce cation channels in Spodoptera frugiperda brush border membranes in suspension and in planar bilayers. FEBS Lett. 360, 217-222.