Gene function analysis by amber stop codon suppression: CMBF is a nuclear protein that supports growth and development of Dictyostelium amoebae

Journal of Molecular Biology

Volumn 305, Issue 4, 2001, Pages 703-714

  Winckler, Thomas ^a   Trautwein, Christine ^b   Tschepke, Christina ^a   Neuhäuser, Christin ^a   Zündorf, Ilse ^a   Beck, Peter ^a   Vogel, Günter ^b   Dingermann, Theodor ^a  

^a GOETHE UNIVERSITY   (Germany)

^b UNIVERSITY OF WUPPERTAL   (Germany)

Author keywords

Dictyostelium; Gene expression; Retrotransposon; Suppression; tRNA

Indexed keywords

DNA BINDING PROTEIN; NUCLEAR PROTEIN; PROTEIN CMBF; TRANSFER RNA; UNCLASSIFIED DRUG;

ARTICLE; CELL GROWTH; CELL NUCLEUS; CELL PROLIFERATION; CONTROLLED STUDY; DICTYOSTELIUM DISCOIDEUM; GENE FUNCTION; GENE REPRESSION; NONHUMAN; PHAGOCYTOSIS; PHENOTYPE; PINOCYTOSIS; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RETROPOSON; STOP CODON; UNTRANSLATED REGION;

DICTYOSTELIUM; DICTYOSTELIUM AMOEBAE; DICTYOSTELIUM DISCOIDEUM;

EID: 0035951309     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4341     Document Type: Article

References (26)

1. The age and evolution of non-LTR retrotransposable elements
2. Non-LTR retrotransposons with unique integration preferences downstream of Dictyostelium discoideum transfer RNA genes
3. Internally located and oppositely oriented polymerase II promoters direct convergent transcription of a LINE-like retroelement, the Dictyostelium repetitive element, from Dictyostelium discoideum
4. Nuclear protein factor binds specifically to the 3′-regulatory module of the long-interspersed-nuclear-element-like Dictyostelium repetitive element
5. A Dictyostelium protein binds to distinct oligo(dA)-oligo(dT) DNA sequences in the C-module of the retrotransposable element DRE
6. Analysis of gene function in Dictyostelium
7. Nonsense suppression in Dictyostelium discoideum
8. Establishment of a system for conditional gene expression using an inducible tRNA suppressor gene
9. Nuclear targeting sequences: A consensus?
10. Nuclear protein localization
11. Isolation of transcription factor IIIC from Dictyostelium discoideum
12. Mechanism of phagocytosis in Dictyostelium discoideum: Phagocytosis is mediated by different recognition sites as disclosed by mutants with altered phagocytotic properties
13. Cytokinesis is defective in Dictyostelium mutants with altered phagocytic recognition, adhesion, and vegetative cell cohesion properties
14. The G protein β subunit is essential for multiple responses to chemoattractants in Dictyostelium
15. G protein β subunit-null mutants are impaired in phagocytosis and chemotaxis due to inappropriate regulation of the actin cytoskeleton
16. A Dictyostelium mutant with defective aggregate size determination
17. A cell-counting factor regulating structure size in Dictyostelium
18. The Dictyostelium MAP kinase kinase DdMEK1 regulates chemotaxis and is essential for chemoattractant-mediated activation of guanylyl cyclase
19. Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture
20. DNA-mediated transformation in Dictyostelium discoideum: Regulated expression of an actin gene fusion
21. A simple cytotoxicity assay using the eucaryotic microorganism Dictyostelium discoideum
22. Optimization and in situ detection of Escherichia coli β-galactosidase gene expression in Dictyostelium discoideum
23. pISAR, a tool for cloning genomic sequences adjacent to the site of vector integration
24. Generation and characterization of monoclonal antibodies against the pyrrolizidine alkaloid retrorsine
25. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
26. Electroblotting of multiple gels: A simple apparatus without buffer tank or rapid transfer of proteins from polyacrylamide to nitrocellulose