Transcriptional activator of phage Φ29 late promoter: Mapping of residues involved in interaction with RNA polymerase and in DNA bending

Molecular Microbiology

Volumn 20, Issue 2, 1996, Pages 273-282

  Mencía, Mario ^a   Monsalve, María ^a   Salas, Margarita ^a   Rojo, Fernando ^a,b  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b CSIC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; RNA POLYMERASE;

ARTICLE; BACILLUS SUBTILIS; BACTERIOPHAGE; CONTROLLED STUDY; DNA BINDING; GENE EXPRESSION REGULATION; NONHUMAN; PRIORITY JOURNAL; TRANSCRIPTION REGULATION;

BACILLUS PHAGES; BASE SEQUENCE; BINDING SITES; CHROMOSOME MAPPING; DNA, VIRAL; DNA-DIRECTED RNA POLYMERASES; GENE EXPRESSION REGULATION, VIRAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID CONFORMATION; PROMOTER REGIONS (GENETICS); SIGMA FACTOR; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS; VIRAL PROTEINS;

EID: 0029925824     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2958.1996.tb02616.x     Document Type: Article

References (23)

1. Aggarwal, A.K., Rodgers, D.W., Drottar, M., Ptashne, M., and Harrison, S.C. (1988) Recognition of a DNA operator by the repressor of phage 434: a view at high resolution. Science 242: 899-907.
2. Barthelemy, I., and Salas, M. (1989) Characterization of a new prokaryotic transcriptional activator and its DNA recognition site. J Mol Biol 208: 225-232.
3. Barthelemy, I., Lázaro, J.M., Méndez, E., Mellado, R.P., and Salas, M. (1987) Purification in an active form of the phage Φ29 protein p4 that controls the viral late transcription. Nucl Acids Res 15: 7781-7793.
4. Carey, J. (1988) Gel retardation at low pH resolves the trp repressor-DNA complexes for quantitative study. Proc Natl Acad Sci USA 85: 975-979.
5. Escarmís, C., and Salas, M. (1982) Nucleotide sequence of the early genes 3 and 4 of bacteriophage Φ29. Nucl Acids Res 10: 5785-5798.
6. Herlitze, S., and Koenen, M. (1990) A general rapid mutagenesis method using polymerase chain reaction. Gene 91: 143-147.
7. Lowry, O.H., Rosenbrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275.
8. Mencía, M., Salas, M., and Rojo, F. (1993) Residues of the Bacillus subtilis phage Φ29 transcriptional activator required both to interact with RNA polymerase and to activate transcription. J Mol Biol 233: 695-704.
9. Monsalve, M., Mencía, M., Rojo, F., and Salas, M. (1995) Transcription regulation in bacteriophage Φ29: expression of the viral promoters throughout the infection cycle. Virology 207: 23-31.
10. Nuez, B., Rojo, F. , Barthelemy, I., and Salas, M. (1991) Identification of the sequences recognized by phage Φ29 transcriptional activator: possible interaction between the activator and the RNA polymerase. Nucl Acids Res 19: 2337-2342.
11. Nuez, B., Rojo, F., and Salas, M. (1992) Phage Φ29 regulatory protein p4 stabilizes the binding of the RNA polymerase to the late promoter in a process involving direct protein-protein contacts. Proc Natl Acad Sci USA 89: 11401-11405.
12. Orchard, K., and May, G.E. (1993) An EMSA-based method for determining the molecular weight of a protein-DNA complex. Nucl Acids Res 21: 3335-3336.
13. Rojo, F., and Salas, M. (1991) A DNA curvature can substitute phage Φ29 regulatory protein p4 when acting as a transcriptional repressor. EMBO J 10: 3429-3438.
14. Rojo, F., Zaballos, A., and Salas, M. (1990) Bend induced by phage Φ29 transcriptional activator in the viral late promoter is required for activation. J Mol Biol 211: 713-725.
15. Schultz, S.C., Shields, G.C., and Steitz, T.A. (1991) Crystal structure of a CAP-DNA complex: the DNA is bent by 90. Science 253: 1001-1007.
16. Serrano, M., Barthelemy, I., and Salas, M. (1991) Transcription activation at a distance by phage Φ29 protein p4. Effect of bent and non-bent intervening DNA sequences. J Mol Biol 219: 403-414.
17. Sogo, J.M., Inciarte, M.R., Corral, J., Viñuela, E., and Salas, M. (1979) RNA polymerase binding sites and transcription of the DNA of Bacillus subtilis phage Φ29. J Mol Biol 127: 411-436.
18. Strauss, J.K., and Maher, L.J. (1994) DNA bending by asymmetric phosphate neutralization. Science 266: 1829-1834.
19. Thompson, J.F., and Landy, A. (1988) Empirical estimation of protein-induced DNA bending angles: applications to λ site-specific recombination complexes. Nucl Acids Res 16: 9687-9705.
20. Wolberger, C., Dong, Y., Ptashne, M., and Harrison, S. (1988) Structure of a phage 434 Cro/DNA complex. Nature 335: 789-795.
21. Yang, C.C., and Nash, H.A. (1989) The interaction of E. coli IHF protein with its specific binding sites. Cell 57: 869-880.
22. Zaballos, A., Salas, M., and Mellado, R.P. (1987) A set of expression plasmids for the synthesis of fused and unfused polypeptides in Escherichia coli. Gene 58: 67-76.
23. Zaballos, A., Salas, M., and Mellado, R.P. (1988) Initiation of phage Φ29 DNA replication by mutants with deletions at the amino end of the terminal protein. Gene 63: 113-121.