Crystallization and preliminary X-ray diffraction analysis of glutaryl 7-aminocephalosporanic acid acylase from Pseudomonas sp. GK16

Journal of Structural Biology

Volumn 131, Issue 1, 2000, Pages 79-81

  Kwon, Taek H ^a   Rhee, Sangkee ^a   Lee, Young S ^a   Park, Sung S ^a   Kim, Kyung H ^a  

^a Korea University   (South Korea)

Author keywords

Glutaryl 7 aminocephalosporanic acid acylase; Protein crystallization; Pseudomonas sp. GK16; X ray diffraction

Indexed keywords

BACTERIAL ENZYME; CALCIUM CHLORIDE; CEPHALOSPORANIC ACID DERIVATIVE; GLUTARYL 7 AMINOCEPHALOSPORANIC ACID ACYLASE; MACROGOL; UNCLASSIFIED DRUG;

ARTICLE; CRYSTAL STRUCTURE; CRYSTALLIZATION; DRUG SYNTHESIS; ENZYME ANALYSIS; ENZYME SUBUNIT; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS; X RAY DIFFRACTION;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS SP.;

EID: 0033816559     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsbi.2000.4256     Document Type: Article

References (15)

1. A protein catalytic framework with an N-terminal nucleophile is capable of self-activation
2. Two-step immobilized enzyme conversion of cephalosporin C to 7-aminocephalosporanic acid
3. Concerning the preparation of 7-amino-cephalosporanic acid
4. The isolation and properties of Pseudomonas mutants with an enhanced productivity of 7β-(4-carboxybutanamido)-cephalosporanic acid acylase
5. Structure and expression of cDNA for D-amino acid oxidase active against cephalosporin C from Fusarium solani
6. Sparse matrix sampling: A screening method for crystallization of proteins
7. Two-step autocatalytic processing of the glutaryl 7-aminocephalosporanic acid acylase from Pseudomonas sp. strain GK16
8. Cloning and characterization of GL-7ACA acylase gene from Pseudomonas sp. GK16
9. Molecular cloning and structure of the gene for 7β-(4-carboxybutanamido) cephalosporanic acid acylase gene from a Pseudomonas strain
10. Solvent content of protein crystals
11. Chemistry of cephalosporin antibiotics. XIV. The reaction of cephalosporin C with nitrosyl chloride
12. Processing of X-ray diffraction data collected in oscillation mode
13. Oxidative modification of a cephalosporin C acylase from Pseudomonas strain N176 and site-directed mutagenesis of the gene
14. Monocyclic β-lactam antibiotics produced by bacteria