Effect of the co-expression of methyltransferase activity on extracellular production of Taq I restriction endonuclease in recombinant E. coli cells

Process Biochemistry

Volumn 37, Issue 5, 2001, Pages 527-534

  Toksoy, Ebru ^a   Krdar, Betül ^a  

^a BOGAZICI UNIVERSITY   (Turkey)

Author keywords

Escherichia coli; Fusion protein; MBP; Methyltransferase; Secretion; Taq I

Indexed keywords

HYBRID PROTEIN; METHYLTRANSFERASE; RESTRICTION ENDONUCLEASE; TAQ POLYMERASE; TETRACYCLINE;

ANIMAL CELL; ANTIBIOTIC RESISTANCE; ARTICLE; BIOREACTOR; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXTRACELLULAR SPACE; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PLASMID; PROMOTER REGION;

EID: 0035924193     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0032-9592(01)00231-X     Document Type: Article

References (21)

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3. The corrected nucleotide sequences of the Taq I restriction and modification enzymes reveal a thirteen-codon overlap
4. Overproduction, purification and crystallization of Taq I restriction endonuclease
5. Influence of stringent and relaxed response on excretion of recombinant proteins and fatty acid composition in Escherichia coli
6. Use of bacteriocin release protein in E. coli for excretion of human growth hormone into the culture medium
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11. Production of active chimeric pediocin AcH in Escherichia coli in the absence of processing and secretion genes from the Pediococcus pap operon
12. High level secretion of recombinant staphylokinase into periplasm of Escherichia coli
13. TolAIII co-overexpression facilitates the recovery of periplasmic recombinant proteins into the growth medium of Escherichia coli
14. High level secretion of Taq I restriction endonuclease by recombinant Escherichia coli
15. High efficiency transformation of E. coli by high voltage electroporation
16. Factors affecting the fermentative production of a lysozyme binding antibody fragment in Escherichia coli
17. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
18. A genetic system for isolation and characterization of Taq I restriction endonuclease mutants
19. Overproduction of EcoR I and SpA::EcoR I fusion protein by Escherichia coli employing the combinations of three different types of multicopy plasmids: Comparison of plasmid stabilities and product concentrations
20. Recombinant protein expression in Escherichia coli