Production of active chimeric pediocin AcH in Escherichia coli in the absence of processing and secretion genes from the Pediococcus pap operon

Applied and Environmental Microbiology

Volumn 64, Issue 1, 1998, Pages 14-20

  Miller, Kurt W ^a   Schamber, Robin ^a   Chen, Yanling ^a   Ray, Bibek ^a  

^a UNIVERSITY OF WYOMING   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BACTERIOCIN; OUTER MEMBRANE PROTEIN; PEDIOCIN;

ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIAL OUTER MEMBRANE; CHIMERA; CYTOPLASM; ESCHERICHIA COLI; NONHUMAN; PEDIOCOCCUS; PEPTIDE ANALYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SECRETION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PEDIOCOCCUS; POSIBACTERIA;

EID: 0031985197     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.1.14-20.1998     Document Type: Article

References (50)

1. Abee, T. 1995. Pore-forming bacteriocins of Gram-positive bacteria and self-protection mechanisms of producer organisms. FEMS Microbiol. Lett. 129:1-10.
2. Amann, E., J. Brosius, and M. Ptashne. 1983. Vectors hearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25:167-178.
3. Bhunia, A., M. C. Johnson, and B. Ray. 1987. Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulphate-polyacrylamide gel electrophoresis. J. Ind. Microhiol. 2:319-322.
4. Bilgin, N., J. I. Lee, H.-Y. Zhu, R. Dalbey, and G. von Heijne. 1990. Mapping of catalytically important domains in Escherichia coli leader peptidase. EMBO J. 9:2717-2722.
5. Braun, V., and K. Rehn. 1969. Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coll cell wall. The specific effect of trypsin on the membrane structure. Eur. J. Biochem. 10:426-438.
6. Bukhtiyarova, M., R. Yang, and B. Ray. 1994. Analysis of the pediocin AcH gene cluster from plasmid pSMB74 and its expression in a pediocin-negative Pediococcus acidilactici strain. Appl. Environ. Microbiol. 60:3405-3408.
7. Chen, Y., R. Shapira, M. Eisenstein, and T. J. Montville. 1997. Functional characterization of pediocin PA-1 binding to liposomes in the absence of a protein receptor and its relationship to a predicted tertiary structure. Appl. Environ. Microbiol. 63:524-531.
8. di Guan, C., P. Li, P. D. Riggs, and H. Inouye. 1988. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene 67:21-30.
9. Eckert, B., and C. F. Beck. 1989. Overproduction of transposon Tn10-encoded tetracycline resistance protein results in cell death and loss of membrane potential. J. Bacteriol. 171:3557-3559.
10. Fath, M. J., and R. Kolter. 1993. ABC-transporters: bacterial exporters. Microhiol. Rev. 57:995-1017.
11. Fimland, G., O. R. Blingsmo, K. Sletten, G. Jung, I. F. Nes, and J. Nissen-Meyer. 1996. New biologically active hybrid bacteriocins constructed by combining regions from various pediocin-like bacteriocins: the C-terminal region is important for determining specificity. Appl. Environ. Microbiol. 62:3313-3318.
12. Haima, P., D. van Sinderen, H. Schotting, S. Bron, and G. Venema. 1990. Development of a β-galactosidase α-complementation system for molecular cloning in Bacillus subtilis. Gene 86:63-69.
13. Hastings, J. W., M. Sailer, K. Johnson, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1991. Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. J. Bacteriol. 173:7491-7500.
14. Havarstein, L. S., H. Holo, and I. F. Nes. 1994. The leader peptide of colicin V shares consensus sequences with leader peptides that are common among peptide bacteriocins produced by Gram-positive bacteria. Microbiology 140: 2383-2389.
15. Hook, V. Y. H., K. Moran, R. Kannan, A. Kohn, M. O. Lively, A. Azaryan, M. Schiller, and K. Miller. High level expression of the prohormones proenkephalin, pro-neuropeptide Y, proopiomelanocortin, and β-protachykinin for in vitro prohormone processing. Protein Express. Purification, in press.
16. Ichihara, S., Y. Matsubara, C. Kato, K. Akasaka, and S. Mizushima. 1993. Molecular cloning, sequencing, and mapping of the gene encoding protease I and characterization of proteinase and proteinase-defective Escherichia coli mutants. J. Bacteriol. 175:1032-1037.
17. Jack, R. W., J. R. Tagg, and B. Ray. 1995. Bacteriocins of gram-positive bacteria. Microbiol. Rev. 59:171-200.
18. Kalchayanand, N., M. B. Hanlin, and B. Ray. 1992. Sublethal injury makes Gram-negative and resistant Gram-positive bacteria sensitive to the bacteriocins, pediocin AcH and nisin. Lett. Appl. Microbiol. 15:239-243.
19. Klaenhammer, T. R. 1993. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12:39-86.
20. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
21. Liu, W., and J. N. Hansen. 1992. Enhancement of the chemical and antimicrobial properties of subtilin by site-directed mutagenesis. J. Biol. Chem. 267:25078-25085.
22. Lopes, J., S. Gottfried, and L. Rothfield. 1972. Leakage of periplasmic enzymes by mutants of Escherichia coli and Salmonella typhimurium: isolation of "periplasmic leaky" mutants. J. Bacteriol. 109:520-525.
23. Marugg, J. D., C. F. Gonzalez, B. S. Kunka, A. M. Ledeboer, M. J. Pucci, M. Y. Toonen, S. A. Walker, L. C. M. Zoetmulder, and P. A. Vandenbergh. 1992. Cloning, expression, and nucleotide sequence of genes involved in production of pediocin PA-1, a bacteriocin from Pediococcus acidilactici PAC1.0. Appl. Environ. Microbiol. 58:2360-2367.
24. Miller, K. W., P. L. Konen, J. Olson, and K. M. Ratanavanich. 1993. Membrane protein topology determination by proteolysis of maltose binding protein fusions. Anal. Biochem. 215:118-128.
25. Moll, G. N., G. C. K. Roberts, W. N. Konings, and A. J. M. Driessen. 1996. Mechanism of lantibiotic-induced pore-formation. Antonie Leeuwenhoek 69:185-191.
26. Moll, G. N., J. Clark, W. C. Chan, B. W. Bycroft, G. C. K. Roberts, W. N. Konings, and A. J. M. Driessen. 1997. Role of transmembrane pH gradient and membrane binding in nisin pore formation. J. Bacteriol. 179:135-140.
27. Motlagh, A. M., M. Bukhtiyarova, and B. Ray. 1994. Complete nucleotide sequence of pSMB74, a plasmid encoding the production of pediocin AcH in Pediococcus acidilactici. Lett. Appl. Microbiol. 18:305-312.
28. Neu, H. C., and L. A. Heppel. 1965. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J. Biol. Chem. 240:3685-3692.
29. Randall, L. L., and S. J. S. Hardy. 1984. Export of protein in bacteria. Microbiol. Rev. 48:290-298.
30. Ray, B. 1992. Pediocin(s) of Pediococcus acidilactici as food biopreservatives, p. 265-322. In B. Ray and M. A. Daeschul (ed.), Food biopreservatives of microbial origin. CRC Press, Inc., Boca Raton, Fla.
31. Ray, B. 1993. Sublethal injury, bacteriocins, and food microbiology. ASM News 59:285-291.
32. Ray, B. 1996. Characteristics and application of pediocin(s) of Pediococcus acidilactici: pediocin PA-1/AcH, p. 155-203. In T. F. Bozuglu and B. Ray (ed.), Lactic acid bacteria: current advances in metabolism, genetics, and applications. Springer, New York, N.Y.
33. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
34. Schagger, H., and G. von Jagow. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368-379.
35. Schatz, P. J., and J. Beckwith. 1990. Genetic analysis of protein export in Escherichia coli. Annu. Rev. Genet. 24:215-248.
36. Siegers, K., A. Heinzmann, and K.-D. Entian. 1996. Biosynthesis of lantibiotic nisin. J. Biol. Chem. 271:12294-12301.
37. Simonen, M., and I. Palva. 1993. Protein secretion in Bacillus species. Microbiol. Rev. 57:109-137.
38. Strauch, K. L., K. Johnson, and J. Beckwith. 1989. Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature. J. Bacteriol. 171:2689-2696.
39. Studier, F. S., A. H. Rosenberg, J. J. Dunn, and J. W. Dubendorff. 1990. Use of T7 RNA polymerase to direct the expression of cloned genes. Methods Enzymol. 185:60-89.
40. Tichaczek, P. S., R. F. Vogel, and W. Hammes. 1994. Cloning and sequencing sakP encoding sakacin P, the bacteriocin produced by Lactobacillus sake LTH673. Microbiology 140:361-367.
41. Valenta, C., A. Bernkop-Schnurch, and H. P. Rigler. 1996. The antistaphy-lococcal effect of nisin in a suitable vehicle: a potential therapy for atopic dermatitis in man. J. Pharm. Pharmacol. 48:988-991.
42. Venema, K., J. Kok, J. D. Marugg, M. Y. Toonen, A. M. Ledeboer, and G. Venema. 1995. Functional analysis of the pediocin operon of Pediococcus acidilactici PAC 1.0: PedB is the immunity protein and PedD is the precursor processing enzyme. Mol. Microbiol. 17:515-522.
43. von Heijne, G. 1985. Signal sequences. The limits of variation. J. Mol. Biol. 184:99-105.
44. Weiss, J. B., and P. J. Bassford, Jr. 1990. The folding properties of the Escherichia coli maltose-binding protein influence its interaction with SccB in vitro. J. Bacteriol. 172:3023-3029.
45. Wickner, W., and M. R. Leonard. 1996. Escherichia coli preprotein translocase. J. Biol. Chem. 271:29514-29516.
46. Worobo, R. W., M. J. van Belkum, M. Sailer, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1995. A signal peptide secretion-dependent bacteriocin from Carnobacterium divergens. J. Bacteriol. 177:3143-3149.
47. Yang, R., M. C. Johnson, and B. Ray. 1992. Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl. Environ. Microbiol. 58:3355-3359.
48. Yem, D. W., and H. C. Wu. 1978. Physiological characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein. J. Bacteriol. 133:1419-1426.
49. Zhang, L. H., M. J. Fath, H. K. Mahanty, P. C. Tai, and R. Kolter. 1995. Genetic analysis of the colicin V secretion pathway. Genetics 141:25-32.
50. Zhen, L., and R. T. Swank. 1993. A simple and high yield method for recovering DNA from agarose gels. BioTechniques 14:894-898.