DegP-coexpression minimizes inclusion-body formation upon overproduction of recombinant penicillin acylase in Escherichia coli

Biotechnology and Bioengineering

Volumn 73, Issue 6, 2001, Pages 484-492

  Lin, Wen Jer ^a   Huang, Shin Wei ^a   Chou, C Perry ^a  

^a FENG CHIA UNIVERSITY   (Taiwan)

Author keywords

DegP; Escherichia coli; Inclusion body; Penicillin acylase; Periplasm; Protease; Proteolysis

Indexed keywords

ENZYMES; ESCHERICHIA COLI; GENES;

INTRACELLULAR CONCENTRATIONS;

BIOTECHNOLOGY;

PENICILLIN ACYLASE; PENICILLIN DERIVATIVE; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM CULTURE; CELL INCLUSION; CYTOPLASM; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SYNTHESIS; ESCHERICHIA COLI; FUNGUS CULTURE; GENE EXPRESSION; NONHUMAN; PROMOTER REGION; PROTEIN AGGREGATION; PROTEIN DEGRADATION; PROTEIN PROCESSING;

BACTERIAL PROTEINS; ENZYME PRECURSORS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HEAT-SHOCK PROTEINS; INCLUSION BODIES; PENICILLIN AMIDASE; PERIPLASMIC PROTEINS; PLASMIDS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES;

ESCHERICHIA COLI;

EID: 0035919127     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1083     Document Type: Article

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