The desymmetrization of bicyclic β-diketones by an enzymatic retro-Claisen reaction. A new reaction of the crotonase superfamily

Journal of Biological Chemistry

Volumn 276, Issue 16, 2001, Pages 12565-12572

  Grogan, Gideon ^a   Roberts, Gareth A ^a   Bougioukou, Despina ^a   Turner, Nicholas J ^a   Flitsch, Sabine L ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIODEGRADATION; CATALYSIS; ENZYME KINETICS; GENETIC ENGINEERING; KETONES; MASS SPECTROMETRY;

DESYMMETRIZATION; HOMOLOGY;

BIOCHEMISTRY;

1,3 DIKETONE; 6 OXOCAMPHOR; 6 OXOCAMPHOR HYDROLASE; ALPHA CAMPHOLINIC ACID; CAMPHOR; DIKETONE; ENOYL COENZYME A HYDRATASE; FERREDOXIN NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE REDUCTASE; HYDROLASE; PROTEIN; UNCLASSIFIED DRUG; 6-OXOCAMPHOR HYDROLASE; PROTEIN SUBUNIT; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GROWTH; BIODEGRADATION; CARBON SOURCE; CLAISEN CONDENSATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME PURIFICATION; GENETIC CODE; MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN STRUCTURE; RETRO CLAISEN REACTION; RHODOCOCCUS; RNA TRANSLATION; SEQUENCE HOMOLOGY; WILD TYPE; BIOREMEDIATION; CHEMISTRY; COMPARATIVE STUDY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN SYNTHESIS; SEQUENCE ALIGNMENT;

BACTERIA (MICROORGANISMS); DRYOBALANOPS; RHODOCOCCUS; RHODOCOCCUS SP.; RHODOCOCCUS SP. NCIMB 9784;

AMINO ACID SEQUENCE; BASE SEQUENCE; BIODEGRADATION, ENVIRONMENTAL; CAMPHOR; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; ENOYL-COA HYDRATASE; FERREDOXIN-NADP REDUCTASE; HYDROLASES; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OPEN READING FRAMES; PROTEIN BIOSYNTHESIS; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; RHODOCOCCUS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 0035918262     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M011538200     Document Type: Article

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