Protein secretion biotechnology using Streptomyces lividans: Large-scale production of functional trimeric tumor necrosis factor α

Biotechnology and Bioengineering

Volumn 72, Issue 6, 2001, Pages 611-619

  Pozidis, Charalambos ^a   Lammertyn, Elke ^b   Politou, Anastasia S ^c   Anné, Jozef ^b   Tsiftsoglou, Asterios S ^d   Sianidis, Giorgos ^a   Economou, Anastassios ^a  

^a INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY   (Greece)

^b REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

^c UNIVERSITY OF CRETE   (Greece)

^d ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

Author keywords

Protein translocase; Secretion; Signal peptide; Streptomyces; Tumor necrosis factor alpha

Indexed keywords

CARRIER PROTEIN; GLUCOSE; HYBRID PROTEIN; OLIGOMER; SIGNAL PEPTIDE; TUMOR NECROSIS FACTOR ALPHA;

APOPTOSIS; ARTICLE; BIOPROCESS; BIOTECHNOLOGY; CIRCULAR DICHROISM; CULTURE MEDIUM; CYTOKINE PRODUCTION; NONHUMAN; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEIN SECRETION; STREPTOMYCES LIVIDANS; TECHNIQUE; THERMOSTABILITY;

BIOREACTORS; CELL CULTURE TECHNIQUES; CULTURE MEDIA; FEASIBILITY STUDIES; FERMENTATION; GLUCOSE; PILOT PROJECTS; POLYMERS; QUALITY CONTROL; RECOMBINANT PROTEINS; STREPTOMYCES; TUMOR NECROSIS FACTOR-ALPHA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MURINAE; POSIBACTERIA; STREPTOMYCES; STREPTOMYCES LIVIDANS; STREPTOMYCES VENEZUELAE;

EID: 0035916869     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20010320)72:6<611::AID-BIT1026>3.0.CO;2-0     Document Type: Article

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