Modifications of Streptomyces signal peptides and their effects on protein production and secretion

FEMS Microbiology Letters

Volumn 160, Issue 1, 1998, Pages 1-10

  Lammertyn, Elke ^a   Anné, Jozef ^a  

^a REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

Author keywords

Protein secretion; Signal peptide; Streptomyces

Indexed keywords

BACTERIAL PROTEIN; MUTANT PROTEIN; PROTEIN PRECURSOR; SIGNAL PEPTIDASE; SIGNAL PEPTIDE; TENDAMISTAT;

AMINO TERMINAL SEQUENCE; CARBOXY TERMINAL SEQUENCE; DNA TRANSCRIPTION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROCESSING; PROTEIN SECRETION; PROTEIN SYNTHESIS; REVIEW; RNA TRANSLATION; STREPTOMYCES; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROTEIN SORTING SIGNALS; STREPTOMYCES;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); STREPTOMYCES; UNCULTURED ACTINOMYCETE;

EID: 0032032192     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00583-1     Document Type: Review

References (27)

1. Akita, M., Sasaki, S., Matsuyama, S.-i. and Mizushima, S. (1990) SecA interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli. J. Biol. Chem. 265, 8164-8169.
2. Borchert, T.V. and Nagarajan, V. (1991) Effect of signal sequence alterations on export of levansucrase in Bacillus subtilis. J. Bacteriol. 173, 276-282.
3. Chang, S.-C., Su, M.-H. and Lee, Y.-H.W. (1997) Roles of the signal peptide and mature domains in the secretion and maturation of the neutral metalloprotease from Streptomyces cacaoi. Biochem. J. 321, 29-37.
4. Chen, M. and Nagarajan, V. (1994) Effect of alteration of charged residues at the N-termini of signal peptides on protein export in Bacillus subtilis. J. Bacteriol. 176, 5796-5801.
5. De Gier, J.-W.L., Valent, G.A., Von Heijne, G. and Luirink, J. (1997) The E. coli SRP: preferences of a targetting factor. FEBS Lett. 408, 1-4.
6. Fass, S.H. and Engels, J.W. (1996) Influence of specific signal peptide mutations on the expression and secretion of the alpha-amylase inhibitor tendamistat in Streptomyces lividans. J. Biol. Chem. 271, 15244-15252.
7. Gennity, J., Goldstein, J. and Inouye, M. (1990) Signal peptide mutants of Escherichia coli. J. Bioenerg. Biomembr. 22, 233-269.
8. Gilbert, M., Morosoli, R., Shareck, F. and Kluepfel, D. (1995) Production and secretion of proteins of Streptomycetes. Crit. Rev. Biotechnol. 15, 13-39.
9. Hale, V.A. and Schottel, J.L. (1996) Mutational analysis of the Streptomyces scabies esterase signal peptide. Appl. Microbiol. Biotechnol. 45, 189-198.
10. Izard, J.W. and Kendall, D.A. (1994) Signal peptides: exquisitely designed transport promoters. Mol. Microbiol. 13, 765-773.
11. Izard, J.W., Rush, S.L. and Kendall, D.A. (1996) The amino-terminal charge and core region hydrophobicity interdependently contribute to the function of the signal sequences. J. Biol. Chem. 271, 21579-21582.
12. Lammertyn, E., Van Mellaert, L., Schacht, S., Dillen, C., Sablon, E., Van Broekhoven, A. and Anné, J. (1997) Evaluation of a novel subtilisin inhibitor gene and mutant derivatives for the expression and secretion of mouse tumour necrosis factor alpha by Streptomyces lividans. Appl. Environ. Microbiol. 63, 1808-1813.
13. Lammertyn, E., Desmyter, S., Schacht, S., Van Mellaert, L. and Anné, J. (1998) Effect of charge variation in the Streptomyces venezuelae α-amylase signal peptide on heterologous protein production by Streptomyces lividans. Appl. Microbiol. Biotechnol., in press.
14. Leu, W.-M., Chen, L.-Y., Liaw, L.-L. and Wu Lee, Y.-H. (1992) Secretion of the Streptomyces tyrosinase is mediated through its trans-activator protein MelC1. J. Biol. Chem. 267, 20108-20113.
15. Mori, H., Araki, M., Hikita, C., Tagaya, M. and Mizushima, S. (1997) The hydrophobic region of signal peptides is involved in the interaction with membrane-bound SecA. Biochim. Biophys. Acta 1326, 23-36.
16. Morosoli, R., Shareck, F. and Kluepfel, D. (1997) Protein secretion in streptomycetes. FEMS Microbiol. Lett. 146, 167-174.
17. Nakamura, K., Fujita, Y., Itoh, Y. and Yamane, K. (1989) Modification of length, hydrophobic properties and electric charge of Bacillus subtilis α-amylase signal peptide and their different effects on the production of secretory proteins in B. subtilis and Escherichia coli cells. Mol. Gen. Genet. 216, 1-9.
18. Nesmeyanova, M.A., Karamyshev, A.L., Karamysheva, Z.N., Kalinin, A.E., Ksenzenko, V.N. and Kajva, A.V. (1997) Positively charged lysine at the N-terminus of the signal peptide of the Escherichia coli alkaline phosphatase provides the secretion efficiency and is involved in the interaction with anionic phospholipids. FEBS Lett. 403, 203-207.
19. Pagé, N., Kluepfel, D., Shareck, F. and Morosoli, R. (1996) Effect of signal peptide alterations and replacement on export of xylanase A in Streptomyces lividans. Appl. Environ. Microbiol. 62, 109-114.
20. Park, J.-S., Horinouchi, S. and Beppu, T. (1991) Characterization of the leader peptide of an Endo-type cellulase produced by an alkalophilic Streptomyces strain. Agric. Biol. Chem. 55, 1745-1750.
21. Phoenix, D.A., Kusters, R., Hikita, C., Mizushima, S., and de Kruijff, B. (1993) OmpF-Lpp signal sequence mutants with varying charge hydrophobicity ratios provide evidence for a phosphatidylglycerol-signal sequence interaction during protein translocation across the Escherichia coli inner membrane. J. Biol. Chem. 268, 17069-17073.
22. Saunders, C.W., Pedroni, J.A. and Monahan, P.M. (1991) Optimization of the signal sequence cleavage site for secretion from Bacillus subtilis of a 34-amino acid fragment of human parathyroid hormone. Gene 102, 177-181.
23. Van Mellaert, L., Lammertyn, E., Schacht, S., Proost, P., Van Damme, J., Wroblowski, B., Anné, J., Scarcez, T., Sablon, E., Raeymaeckers, J. and Van Broekhoven, A. (1998) Molecular characterization of a novel subtilisin inhibitor protein produced by Streptomyces venezuelae CBS 762.70. DNA Sequence, in press.
24. Vigal, T., Gil, J.A., Daza, A., Garcia-Gonzalez, M.D., Villadas, P. and Martin, J.F. (1991) Effects of replacements of promoters and modification of the leader peptide region of the amy gene of Streptomyces griseus on synthesis and secretion of alpha-amylase by Streptomyces lividans. Mol. Gen. Genet. 231, 88-96.
25. Virolle, M.-J., Long, C.M., Chang, S. and Bibb, M.J. (1988) Cloning, characterization and regulation of an alpha-amylase gene from Streptomyces venezuelae. Gene 74, 321-334.
26. von Heijne, G. (1986) The N-C charge imbalance may be important for signal sequence function in bacteria. J. Mol. Biol. 192, 287-290.
27. von Heijne, G. and Abrahmsén, L. (1989) Species-specific variation in signal peptide design: implications for secretion in foreign hosts. FEBS Lett. 244, 439-446.