Engineering the pH-optimum of a triglyceride lipase: From predictions based on electrostatic computations to experimental results

Journal of Biotechnology

Volumn 87, Issue 3, 2001, Pages 225-254

  Neves Petersen, Maria Teresa ^a   Petersen, Evamaria I ^a   Fojan, Peter ^a   Noronha, Melinda ^a,b   Madsen, Rune G ^a   Petersen, Steffen B ^a  

^a AALBORG UNIVERSITY   (Denmark)

^b UNIVERSITY OF LISBON   (Portugal)

Author keywords

Electrostatic interactions; Electrostatic potential; Engineering pH optimum; Lipase esterase pH activity profile; pH optimum shift

Indexed keywords

CALCULATIONS; ENZYMES; PH; TITRATION;

ELECTROSTATIC COMPUTATIONS;

BIOTECHNOLOGY;

ALANINE; ALKALINE EARTH METAL; CUTINASE; ESTER; ESTERASE; GLUTAMINE; LYSINE; PROLINE; THREONINE; TRIACYLGLYCEROL LIPASE;

ARTICLE; ELECTRIC POTENTIAL; ELECTRICITY; ENERGY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; FUSARIUM SOLANI; GENE MUTATION; GENETIC ENGINEERING; MELTING POINT; PH MEASUREMENT; PRIORITY JOURNAL; TEMPERATURE DEPENDENCE; TITRIMETRY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BASE SEQUENCE; CARBOXYLIC ESTER HYDROLASES; CIRCULAR DICHROISM; COMPUTER SIMULATION; ELECTROSTATICS; ENZYME STABILITY; FUSARIUM; HYDROGEN-ION CONCENTRATION; LIPASE; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

FUSARIUM; FUSARIUM SOLANI;

EID: 0035907145     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(01)00240-1     Document Type: Article

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