Multiparameter flow cytometric approach for simultaneous evaluation of T lymphocyte-endothelial cell interactions

Communications in Clinical Cytometry

Volumn 46, Issue 5, 2001, Pages 271-280

  Krupnick, Alexander S ^a   Kreisel, Daniel ^a   Szeto, Wilson Y ^a   Popma, Sicco H ^a   Amin, Kunjlata M ^a   Moore, Jonni S ^a   Rosengard, Bruce R ^a  

^a HOSPITAL OF THE UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

5,6 Carboxyfluorescein diacetate succinimidyl ester; Cytometry; Endothelial cells; Mouse

Indexed keywords

6 CARBOXYFLUORESCEIN; CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; MAJOR HISTOCOMPATIBILITY ANTIGEN; SUCCINIMIDE DERIVATIVE;

ALLOTRANSPLANTATION; ANIMAL CELL; APOPTOSIS; ARTICLE; CELL INTERACTION; CELL PROLIFERATION; COCULTURE; ENDOTHELIUM CELL; FLOW CYTOMETRY; FLUORESCENCE; GRAFT REJECTION; IMMUNOLOGICAL MONITORING; IMMUNOPHENOTYPING; IMMUNOTHERAPY; LYMPHOCYTE DIFFERENTIATION; LYMPHOCYTE PROLIFERATION; MAJOR HISTOCOMPATIBILITY COMPLEX; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE; T LYMPHOCYTE ACTIVATION; T LYMPHOCYTE SUBPOPULATION; XENOTRANSPLANTATION;

ANIMALS; APOPTOSIS; CELL COMMUNICATION; CELL LINE; CELL SIZE; COCULTURE TECHNIQUES; ENDOTHELIUM, VASCULAR; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; HISTOCOMPATIBILITY ANTIGENS CLASS II; HUMANS; MALE; MEMBRANE GLYCOPROTEINS; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; PHENOTYPE; PORE FORMING CYTOTOXIC PROTEINS; SUCCINIMIDES; T-LYMPHOCYTE SUBSETS; T-LYMPHOCYTES, CYTOTOXIC; THYMIDINE; TRANSPLANTATION, HOMOLOGOUS;

EID: 0035888482     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.1168     Document Type: Article

References (40)

1. Antibody-induced transplant arteriosclerosis is prevented by graft expression of antioxidant and anti-apoptotic genes
2. Development of coronary artery disease in cardiac transplant patients receiving immunosuppressive therapy with cyclosporine and prednisone
3. Chronic rejection
4. Interferon-gamma elicits arteriosclerosis in the absence of leukocytes
5. Can graft endothelial cells initiate a host anti-graft immune response?
6. Transplant risks
7. Antigenic specificity of antibody reactive in the antiglobulin-augmented lymphocytotoxicity test
8. One-way stimulation in mixed leukocyte cultures
9. Anti-cd45 monoclonal antibody perfusion of human renal allografts prior to transplantation. A safety and immunohistological study
10. Cardiac allograft vasculopathy. Association with cell-mediated but not humoral alloimmunity to donor-specific vascular endothelium
11. + T cells: A mechanism of immunoregulation?
12. Specific suppression of interleukin 2 biosynthesis by synthetic antisense oligodeoxynucleotides does not influence allograft rejection
13. Cd39 modulates il-1 release from activated endothelial cells
14. Rapid secretion of prestored interleukin 8 from weibel-palade bodies of microvascular endothelial cells
15. The endothelium and cytokine secretion: The role of peroxidases as immunoregulators
16. Radiation induced endothelial cell retraction in vitro: Correlation with acute pulmonary edema
17. Determination of lymphocyte division by flow cytometry
18. Following the fate of individual t cells throughout activation and clonal expansion. Signals from T cell receptor and cd28 differentially regulate the induction and duration of a proliferative response
19. Dynamics and requirements of t cell clonal expansion in vivo at the single-cell level: Effector function is linked to proliferative capacity
20. + T cell precursor frequency
21. A simple method for evaluating the rejection of grafted spleen cells by flow cytometry and tracing adoptively transferred cells by light microscopy
22. Measurement of candida-specific blastogenesis: Comparison of carboxyfluorescein succinimidyl ester labelling of T cells, thymidine incorporation, and CD69 expression
23. Immune monitoring in xenotransplantation: The multiparameter flow cytometric mixed lymphocyte culture assay
24. A simple method to culture mouse vascular endothelium
25. + T cell death
26. A rapid method for the isolation of functional thymus-derived murine lymphocytes
27. Induction of interleukin 4 (IL-4) expression in T helper (th) cells is not dependent on IL-4 from non-th cells
28. + T lymphocytes
29. Novel approach for simultaneous evaluation of cell phenotype, apoptosis, and cell cycle using multiparameter flow cytometry
30. Influence of antigen dose and costimulation on the primary response of cd8+ T cells in vitro
31. Actinobacillus actinomycetemcomitans immunosuppressive protein is a member of the family of cytolethal distending toxins capable of causing a g2 arrest in human T cells
32. Preferential impairment of nitric oxide-mediated endothelium-dependent relaxation in human cervical arteries after irradiation
33. Radiation-induced normal tissue injury: Role of adhesion molecules in leukocyte-endothelial cell interactions
34. Radiation-induced apoptosis of endothelial cells in the murine central nervous system: Protection by fibroblast growth factor and sphingomyelinase deficiency
35. Expression of perforin and cytolytic potential of human peripheral blood lymphocyte subpopulations
36. The cell cycle and induction of apoptosis in a hamster fibrosarcoma cell line treated with anti-cancer drugs: Its importance to solid tumour chemotherapy
37. Tnf-alpha regulation of fas ligand expression on the vascular endothelium modulates leukocyte extravasation
38. Three-color versus four-color multiparameter cell cycle analyses of primary acute myeloid leukemia samples
39. Long-term acceptance of skin and cardiac allografts after blocking CD40 and CD28 pathways