Immune monitoring in xenotransplantation: The multiparameter flow cytometric mixed lymphocyte culture assay

Communications in Clinical Cytometry

Volumn 42, Issue 5, 2000, Pages 277-283

  Popma, Sicco H ^a   Krasinskas, Alyssa M ^a   McLean, Andrew D ^a   Szeto, Wilson Y ^a   Kreisel, Daniel ^a   Moore, Jonni S ^a   Rosengard, Bruce R ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Carboxyfluorescein succinimidyl ester; Multiparameter mixed lymphocyte cultures; Proliferation

Indexed keywords

CELL MARKER; THYMIDINE;

ARTICLE; CELL DIVISION; CELL INTERACTION; CELL PROLIFERATION; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; IMMUNE STATUS; IMMUNOASSAY; IMMUNOLOGICAL MONITORING; LYMPHOCYTE CULTURE; NORMAL HUMAN; PHENOTYPE; PRIORITY JOURNAL; XENOTRANSPLANTATION;

CELL DIVISION; CELLS, CULTURED; COMPARATIVE STUDY; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; HUMAN; LYMPHOCYTE CULTURE TEST, MIXED; LYMPHOCYTES; MITOGENS; MONITORING, IMMUNOLOGIC; REPRODUCIBILITY OF RESULTS; SUCCINIMIDES; TRANSPLANTATION, HETEROLOGOUS;

SUIDAE;

EID: 0034667870     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20001015)42:5<277::AID-CYTO4>3.0.CO;2-A     Document Type: Article

References (31)

1. Xenotransplantation of hDAF-transgenic swine hearts
2. Strategies to prevent thrombosis in xenotransplants
3. Literature update 1998, part 4
4. Stimulation of the primary mixed leukocyte reaction
5. Human anti-porcine xenogeneic T cell response: Evidence for allelic specificity of mixed leukocyte reaction and for both direct and indirect pathways of recognition
6. Stable cell membrane labelling
7. Fluorescent in vivo tracking of hematopoietic cells. Part I. Technical considerations
8. A flow cytometric method to assess antigen-specific proliferative responses of different subpopulations of fresh and cryopreserved human peripheral blood mononuclear cells
9. Cell-mediated cytotoxicity. A highly sensitive and informative flow cytometric assay
10. Homing of fluorescently labeled murine hematopoietic stem cells
11. Mechanisms of adoptive immunotherapy: Improved methods for in vivo tracking of tumor-infiltrating lymphocytes and lymphokine-activated killer cells
12. Determination of lymphocyte division by flow cytometry
13. Tracking alloreactive cell division in vivo
14. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy
15. A simple method for evaluating the rejection of grafted spleen cells by flow cytometry and tracing adoptively transferred cells by light microscopy
16. - cell production in vitro
17. Measurement of Candida-specific blastogenesis: Comparison of carboxyfluorescein succinimidyl ester labelling of T cells, thymidine incorporation, and CD69 expression
18. Following the fate of individual T cells throughout activation and clonal expansion. Signals from T cell receptor and CD28 differentially regulate the induction and duration of a proliferative response
19. Single cell analysis of human allo and xenorecognition pathways mirrors the mixed lymphocyte response
20. Effects of supravital fluorochromes used to analyze the in vivo homing of murine lymphocytes on cellular function
21. Identification of growth inhibited cells by retention of a lipophilic fluorescent dye
22. A novel single-cell proliferation assay shows that long-term culture-initiating cell (LTC-IC) maintenance over time results from the extensive proliferation of a small fraction of LTC-IC
23. A new flow cytometric method for quantitative assessment of lymphocyte mitogenic potentials
24. A novel approach to the measurement of different in vitro leukaemic cell growth parameters: The use of PKH GL fluorescent probes
25. Measurement of the growth parameters of precursor B-acute lymphoblastic leukaemic cells in co-culture with bone marrow stromal cells; detection of two cd10 positive populations with different proliferative capacities and survival
26. Proliferation-induced decline of primitive hematopoietic progenitor cell activity is coupled with an increase in apoptosis of ex vivo expanded CD34+ cells
27. Alloantigen-induced unresponsiveness in cord blood T lymphocytes is associated with defective activation of Ras
28. Use of fluorescent dyes in the determination of adherence of human leucocytes to endothelial cells and the effect of fluorochromes on cellular function
29. The use of 7-amino actinomycin D in identifying apoptosis: Simplicity of use and broad spectrum of application compared with other techniques
30. Studies on the down-regulation of major histocompatibility complex class I gene expression in adenovirus-transformed cells