Subtle differences in dissociation rates of interactions between destabilized human carbonic anhydrase II mutants and immobilized benzenesulfonamide inhibitors probed by a surface plasmon resonance biosensor

Analytical Biochemistry

Volumn 296, Issue 2, 2001, Pages 188-196

  Svedhem, Sofia ^a   Enander, Karin ^a   Karlsson, Martin ^a   Sjöbom, Hans ^b   Liedberg, Bo ^a   Löfås, Stefan ^b   Mårtensson, Lars Göran ^a   Sjöstrand, Sven Erik ^a   Svensson, Stefan ^a   Carlsson, Uno ^a   Lundström, Ingemar ^a  

^a LINKÖPING UNIVERSITY   (Sweden)

^b BIACORE AB   (Sweden)

Author keywords

Benzenesulfonamide; Biacore; Destabilized mutants; Dissociation rate constants; Human carbonic anhydrase II; Kinetics; Molecular dynamics; Surface plasmon resonance

Indexed keywords

BIOSENSORS; DISSOCIATION; ENTROPY; ENZYME KINETICS; KINETICS; MOLECULAR DYNAMICS; PLASMONS; RATE CONSTANTS; RESONANCE; SURFACE PLASMON RESONANCE;

BENZENESULFONAMIDE; BIACORE; DESTABILIZED MUTANTS; DISSOCIATION RATE CONSTANT; HUMAN CARBONIC ANHYDRASE II;

CARBONIC ANHYDRASE;

BENZENESULFONAMIDE DERIVATIVE; CARBONATE DEHYDRATASE II;

ARTICLE; BINDING AFFINITY; BIOSENSOR; DISSOCIATION CONSTANT; ENTROPY; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; POINT MUTATION; PRIORITY JOURNAL; PROTEIN ANALYSIS;

EID: 0035884183     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5301     Document Type: Article

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