Accurate determination of rate constants of very slow, tight-binding competitive inhibitors by numerical solution of differential equations, independently of precise knowledge of the enzyme concentration

Analytical Biochemistry

Volumn 295, Issue 2, 2001, Pages 186-193

  Plesner, Igor W ^a   Bülow, Anne ^a   Bols, Mikael ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

Competitive inhibitors; Differential equations; Enzymes; Rate constant determination; Slow binding; Thermodynamic functions of binding; Transition state kinetics; glucosidase

Indexed keywords

DIFFERENTIAL EQUATIONS; ENZYMES;

GLUCOSIDASE; RATE CONSTANT DETERMINATION; SLOW BINDING; THERMODYNAMIC FUNCTIONS; TRANSITION STATE;

RATE CONSTANTS;

2 PHENETHYLGLUCOIMIDAZOLE 1; 4 NITROPHENYLGALACTOSIDE; BETA GLUCOSIDASE; ENZYME INHIBITOR; FAGOMINE; GLUCOSE DERIVATIVE; IMIDAZOLE DERIVATIVE; ISOFAGOMINE; UNCLASSIFIED DRUG;

ACCURACY; ANALYTIC METHOD; ARTICLE; BINDING COMPETITION; CALCULATION; CONCENTRATION (PARAMETERS); ENZYME BINDING; ENZYME INHIBITOR COMPLEX; ENZYME SUBSTRATE; KINETICS; PARAMETER; PRIORITY JOURNAL; REACTION ANALYSIS; REACTION TIME; RELIABILITY; TEMPERATURE DEPENDENCE; THERMODYNAMICS;

PRUNUS DULCIS;

EID: 0035881787     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5221     Document Type: Article

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