SIN-1-induced DNA damage in isolated human peripheral blood lymphocytes as assessed by single cell gel electrophoresis (comet assay)

Free Radical Biology and Medicine

Volumn 30, Issue 6, 2001, Pages 679-685

  Doulias, Paschalis Thomas ^a   Barbouti, Alexandra ^a   Galaris, Dimitrios ^a   Ischiropoulos, Harry ^b  

^a UNIVERSITY OF IOANNINA   (Greece)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

BAPTA AM; Dehydroascorbic acid; Free radicals; Human lymphocytes; Hydrogen peroxide; SIN 1; Single cell gel electrophoresis (comet assay)

Indexed keywords

ASCORBIC ACID; CALCIUM CHELATING AGENT; CALCIUM ION; CATALASE; DEHYDROASCORBIC ACID; HYDROGEN PEROXIDE; LINSIDOMINE; NITRIC OXIDE; PEROXYNITRITE; SUPEROXIDE; SUPEROXIDE DISMUTASE;

ANALYTIC METHOD; ARTICLE; CALCIUM CELL LEVEL; CALCIUM HOMEOSTASIS; COMET ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA STRAND BREAKAGE; DRUG EFFECT; DRUG EXPOSURE; HUMAN; HUMAN CELL; MONONUCLEAR CELL; NORMAL HUMAN; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL;

ASCORBIC ACID; CALCIUM; CATALASE; CHELATING AGENTS; COMET ASSAY; DATA INTERPRETATION, STATISTICAL; DNA DAMAGE; DNA, SINGLE-STRANDED; DOSE-RESPONSE RELATIONSHIP, DRUG; EGTAZIC ACID; FLOW CYTOMETRY; HUMANS; KINETICS; LYMPHOCYTES; MICROSCOPY, ULTRAVIOLET; MOLSIDOMINE; NITRATES; NITRIC OXIDE DONORS; SUPEROXIDE DISMUTASE;

EID: 0035868469     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(00)00511-6     Document Type: Article

References (44)

1. The reaction rate of nitric oxide with superoxide
2. Biological tyrosine nitration: A pathophysiological function of nitric oxide and reactive oxygen species
3. Oxidative chemistry of nitric oxide: The roles of superoxide, peroxynitrite, and carbon dioxide
4. Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and the ugly
5. Peroxynitrite oxidation of sulfhydryls: The cytotoxic potential of superoxide and nitric oxide
6. Peroxynitrite-induced membrane lipid peroxidation: The cytotoxic potential of superoxide and nitric oxide
7. Formation of 8-nitroguanine by the reaction of guanine with peroxynitrite in vitro
8. Base modification and strand breakage in isolated calf thymus DNA and in DNA from human skin epidermal keratinocytes exposed to peroxynitrite or 3-morpholinosydnonimine
9. Peroxinitrite-induced apoptosis in T84 and RAW264.7 cells: Attenuation by L-ascorbic acid
10. Peroxynitrite-induced cardiac myocyte injury
11. DNA strand breakage, activation of poly(ADP-ribose) synthetase, and cellular energy depletion are involved in the cytotoxicity in macrophages and smooth muscle cells exposed to peroxynitrite
12. Effects of carbon dioxide/bicarbonate on induction of DNA single-strand breaks and formation of 8-oxoguanine and base-propenal mediated by peroxynitrite
13. Oxidative DNA damage induced by simultaneous generation of nitric oxide and superoxide
14. DNA damage by peroxynitrite characterized with DNA repair enzymes
15. Damage to DNA by reactive oxygen and nitrogen species: Role in inflammatory disease and progression to cancer
16. Peroxynitrite causes DNA nicks in plasmid pBR322
17. DNA damage and mutation in human cells exposed to nitric oxide in vitro
18. DNA strand breakage and activation of poly-ADP ribosyltransferase: A cytotoxic pathway triggered by peroxynitrite
19. Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide
20. DNA damage induced by peroxynitrite: Subsequent biological effects
21. Peroxynitrite-induced DNA damage in the supF gene: Correlation with the mutational spectrum
22. Comet assay for nuclear DNA damage
23. 2+-dependent DNA damage in human peripheral blood lymphocytes
24. Endogenous nitric oxide induced by interleukin-1b in rat islets of Langerhans and HIT-T 15 cells causes significant DNA damage as measured by the 'comet' assay
25. Comet assay to detect nitric oxide-dependent DNA damage in mammalian cells
26. Use of the comet assay to investigate possible interactions of nitric oxide and reactive oxygen species in the induction of DNA damage and inhibition of function in an insulin-secreting cell line
27. Superoxide anion generation by human peripheral blood mononuclear cells in response to prothymosin α
28. DNA instability (strand breakage, uracil misincorporation, and defective repair) is increased by folic acid depletion in human lymphocytes in vitro
29. The kinetics of repair of oxidative DNA damage (strand breaks and oxidized pyrimidines) in human cells
30. The comparative toxicity of nitric oxide and peroxynitrite to Escherichia coli
31. Calcium chelator Quin 2 prevents hydrogen peroxide-induced DNA breakage and cytotoxicity
32. Dietary carcinogens and anticarcinogens: Oxygen radicals and degenerative diseases
33. Effective inhibition by b-carotene of cellular DNA breaking induced by peroxynitrous acid
34. Mammalian facilitative hexose transporters mediate the transport of dehydroascorbic acid
35. Efficient transport and accumulation of vitamin C in HL-60 cells depleted of glutathione
36. Accumulation of vitamin C (ascorbate) and its oxidized metabolite dehydroascorbic acid occurs by separate mechanisms
37. Effect of various nitric-donor agents on peroxide mediated toxicity. A direct correlation between nitric oxide formation and protection
38. The chemistry and tumoricidal activity of nitric-oxide, hydrogen peroxide and the implications to cell resistance susceptibility
39. Apoptosis and necrosis: Two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate ot nitric oxide/superoxide in cortical cell cultures
40. Increased cytotoxicity of 3-morpholinosyndonimine to HepG2 cells in the presence of superoxide dismutase
41. Cytotoxicity of nitric oxide in Fu5 rat hepatoma cells: Evidence for co-operative action with hydrogen peroxide
42. Conventional cell culture media do not adequately supply cells with antioxidants and thus facilitate peroxide-induced genotoxicity
43. Aconitase is readily inactivated by peroxynitrite, but not by its precursor nitric oxide
44. Superoxide and peroxynitrite inactivate aconitases, but nitric oxide does not