A cystatin-based affinity procedure for the isolation and analysis of papain-like cysteine proteinases from tissue extracts

Analytical Biochemistry

Volumn 289, Issue 2, 2001, Pages 231-238

  Tombaccini, Donatella ^a   Mocali, Alessandra ^a   Weber, Ekkehard ^b   Paoletti, Francesco ^a  

^a UNIVERSITY OF FLORENCE   (Italy)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Affinity adsorption; Cell extracts; Cultured fibroblasts; Cysteine proteinases; Egg white cystatin; Electrophoresis; Immunoblots; Papain like enzymes

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; CELL CULTURE; CELLS; CHEMICAL ANALYSIS; ELECTROPHORESIS; TISSUE;

AFFINITY ADSORPTION; CELL EXTRACTS; CYSTATINS; CYSTEINE PROTEINASE; IMMUNOBLOTS;

PAPAIN;

CYSTATIN; CYSTEINE PROTEINASE; EGG WHITE; PAPAIN; SEPHAROSE; TISSUE EXTRACT;

ADSORPTION; ANIMAL TISSUE; ARTICLE; BODY FLUID; COMPLEX FORMATION; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ANALYSIS; ENZYME DEGRADATION; ENZYME ISOLATION; HUMAN; HUMAN TISSUE; IMMUNOASSAY; IMMUNOBLOTTING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; RAT;

EID: 0035865223     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4937     Document Type: Article

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