Increased efficacy of antileishmanial antisense phosphorothioate oligonucleotides in Leishmania amazonensis overexpressing ribonuclease H

Biochemical Pharmacology

Volumn 61, Issue 4, 2001, Pages 467-476

  Mishra, Manjari ^a   Bennett, Jabbar R ^a   Chaudhuri, Gautam ^a  

^a MEHARRY MEDICAL COLLEGE   (United States)

Author keywords

Amastigote; Antisense phosphorothioate oligonucleotide; Differentiation; Leishmania; Promastigote; Ribonuclease H

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; COMPLEMENTARY DNA; LUCIFERASE; MESSENGER RNA; PHOSPHOROTHIOIC ACID DERIVATIVE; RIBONUCLEASE H;

ANTIPROTOZOAL ACTIVITY; ARTICLE; CONTROLLED STUDY; DRUG EFFICACY; DRUG MECHANISM; ENZYME ACTIVITY; ENZYME INHIBITION; GENE OVEREXPRESSION; GENETIC TRANSFECTION; LEISHMANIA; LEISHMANIASIS; MACROPHAGE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION;

ANIMALS; ANTIPROTOZOAL AGENTS; BASE SEQUENCE; DNA, PROTOZOAN; DRUG EVALUATION, PRECLINICAL; GENE EXPRESSION; LEISHMANIA; LUCIFERASES; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES, ANTISENSE; RIBONUCLEASE H, CALF THYMUS; RNA, MESSENGER; THIONUCLEOSIDES; TRANSFECTION; TRYPANOSOMA BRUCEI BRUCEI;

EID: 0035865179     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-2952(00)00568-2     Document Type: Article

References (52)

1. Leishmania species: Models of intracellular parasitism
2. Leishmania major Friedlin chromosome 1 has an unusual distribution of protein-coding genes
3. More surprises from Kinetoplastida
4. Test of heterologous promoters and intergenic regions in Leishmania major
5. 5′ Sequences essential for trans-splicing of msp (gp63) RNAs in Leishmania chagasi
6. Antisense phosphorothioate oligonucleotides: Selective killing of the intracellular parasite Leishmania amazonensis
7. Antisense oligonucleotides containing modified bases inhibit in vitro translation of Leishmania amazonensis mRNAs by invading the miniexon hairpin
8. Improved leishmanicidal effect of phosphorothioate antisense oligonucleotides by LDL-mediated delivery
9. Scavenger receptor-mediated delivery of anti-mini-exon antisense phosphorothioate oligonucleotide to Leishmania-infected macrophages. Selective and efficient elimination of the parasite
10. Medicinal chemistry strategies for antisense research
11. Ribonuclease H. An enzyme degrading the RNA moiety of DNA-RNA hybrids
12. Ribonuclease H from K562 human erythroleukemia cells. Purification, characterization, and substrate specificity
13. The subunit structure of calf thymus ribonuclease HI as revealed by immunological analysis
14. Renaturase and ribonuclease H: A novel mechanism that influences transcript displacement by RNA polymerase II in vitro
15. Purification and characterization of human ribonuclease HII
16. Properties of cloned and expressed human RNase H1
17. 2+-dependent RNase HIII from Bacillus subtilis: Classification of RNases H into three families
18. How does RNase H recognize a DNA· RNA hybrid?
19. Three-dimensional structure of ribonuclease H from E. coli
20. Importance of the positive charge cluster in Escherichia coli ribonuclease HI for the effective binding of the substrate
21. Identification of the amino acid residues involved in an active site of Escherichia coli ribonuclease H by site-directed mutagenesis
22. Enzymatic activity and protein stability of E. coli ribonuclease HI
23. Physiological functions of Escherichia coli RNase HI
24. Recombining the structures of HIV integrase, RuvC and RNase H
25. Molecular cloning and expression of cDNA for human RNase H
26. Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI
27. RNase H of retroviral reverse transcriptase
28. Cloning of the cDNA encoding the large subunit of human RNase HI, a homologue of the prokaryotic RNase HII
29. Isolation and characterization of a second RNase H (RNase HII) of Escherichia coli K-12 encoded by the rnhB gene
30. Functional complementation of an Escherichia coli ribonuclease H mutation by a cloned genomic fragment from the trypanosomatid Crithidia fasciculata
31. Molecular cloning and expression of a ribonuclease H from the kinetoplastid, Trypanosoma brucei
32. Disruption of the Crithidia fasciculata RNH1 gene results in the loss of two active forms of ribonuclease H
33. Ribonuclease H of Saccharomyces cerevisae, Schizosaccharomyces pombe, Crithidia fasciculata and Neurospora crassa
34. Trypanosoma brucei RNase HI requires its divergent spacer subdomain for enzymatic function and its conserved RNA binding motif for nuclear localization
35. Surface acid proteinase (gp63) of Leishmania mexicana. A metallo-enzyme capable of protecting liposome-encapsulated proteins from phagolysosomal degradation by macrophages
36. Characterization of developmentally regulated activities in axenic amastigotes of Leishmania donovani
37. Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms
38. Double-targeted gene replacement for creating null mutants
39. Surface Zn-proteinase as a molecule for defense of Leishmania mexicana amazonensis promastigotes from cytolysis inside macrophage phagolysosomes
40. Transfection of Leishmania and Trypanosoma brucei by electroporation
41. Identification and characterization of a transcriptional silencer upstream of the human BRCA2 gene
42. Methods - rnh gene and ribonuclease H activity analysis
43. Selective delivery of drugs to macrophages through a highly specific receptor. An efficient chemotherapeutic approach against leishmaniasis
44. Receptor-mediated drug delivery to macrophages in chemotherapy of leishmaniasis
45. Identification of cis-acting gene regulatory element from the lemdr1 locus of Leishmania enriettii
46. An RNA polymerase II promoter in the hsp70 locus of Trypanosoma brucei
47. Transcription of the Leishmania major Hsp70-I gene locus does not proceed through the non-coding region
48. Initiation of translation in prokaryotes and eukaryotes
49. Transcriptional mapping of the amplified region encoding the dihydrofolate reductase-thymidylate synthase of Leishmania major reveals a high density of transcripts, including overlapping and antisense RNAs
50. Morpholino antisense oligomers: The case for an RNase H-independent structural type