Different suicide inactivation processes for the peroxidase and cyclooxygenase activities of prostaglandin endoperoxide H synthase-1

Biochemical and Biophysical Research Communications

Volumn 289, Issue 4, 2001, Pages 869-875

  Song, Inseok ^a   Ball, Terry M ^b   Smith, William L ^b  

^a University of Seoul   (South Korea)

^b Michigan State University   (United States)

Author keywords

Arachidonic acid; Aspirin; Compound I; Compound II; Cyclooxygenase; Flurbiprofen; Hydroperoxide; Linoleic acid; Peroxidase

Indexed keywords

PEROXIDASE; PROSTAGLANDIN ENDOPEROXIDE H SYNTHASE 1; PROSTAGLANDIN ENDOPEROXIDE H SYNTHASE 2; PROSTAGLANDIN G2; PROSTAGLANDIN SYNTHASE; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME MECHANISM; PRIORITY JOURNAL; PROSTAGLANDIN SYNTHESIS;

EID: 0035861943     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.6071     Document Type: Article

References (27)

1. Cyclooxygenases: Structural, cellular and molecular biology
2. Arachidonic acid oxygenation by COX-1 and COX-2
3. Why there are two cyclooxygenases?
4. Comparison of hydroper-oxide initiator requirements for the cyclooxygenase activities of prostaglandin H synthase-1 and -2
5. Spectral properties of the higher oxidation states of prostaglandin H synthase
6. Higher oxidation states of prostaglandin H synthase. Rapid electronic spectroscopy detected two spectral intermediates during the peroxidase reaction with prostaglandin G2
7. Higher oxidation states of prostaglandin H synthase. EPR study of a transient tyrosyl radical in the enzyme during the peroxidase reaction
8. Prostaglandin H synthase: Kinetics of tyrosyl radical formation and of cyclooxygenase catalysis
9. Tyrosine 385 of prostaglandin endoperoxide synthase is required for cyclooxygenase catalysis
10. On the mechanism of the biosynthesis of prostaglandins E-1 and F-1-alpha
11. Spectroscopic evidence for reaction of prostaglandin H synthase-1 tyrosyl radical with arachidonic acid
12. Stereospecificity of hydrogen abstraction in the conversion of arachidonic acid to 15R-HETE by aspirin-treated cyclooxygenase-2: Implications for the alignment of substrate in the active site
13. A mechanistic study of self-inactivation of the peroxidase activity in prostaglandin H synthase-1
14. Peroxidase self-inactivation in prostaglandin H synthase-1 pretreated with cyclooxygenase inhibitors or substituted with mangano protoporphyrin IX
15. Oxygenation of polyunsaturated fatty acids during prostaglandin biosynthesis by sheep vesicular glands
16. Interaction between peroxidase and cyclooxygenase activities in prostaglandin-endoperoxide synthase. Interpretation of reaction kinetics
17. Attachment of substrate metabolite to prostaglandin H synthase upon reaction with arachidonic acid
18. Covalent binding of arachidonic acid metabolites to human platelet proteins
19. Different catalytically competent arrangements of arachidonic acid within the cyclooxygenase active site of prostaglandin endoperoxide H synthase-1 lead to the formation of different oxygenated products
20. Comparison of branched-chain and tightly coupled reaction mechanisms for prostaglandin H synthase
21. Prostaglandin G2 levels during reaction of prostaglandin H synthase with arachidonic acid
22. Purification and characterization of the human recombinant histidine-tagged prostaglandin endoperoxide H synthases-1 and -2
23. Arachidonic acid and nonsteroidal anti-inflammatory drugs induce conformational changes in the human prostaglandin endoperoxide H2 synthase-2 (cyclooxygenase-2)
24. Nitric oxide trapping of tyrosyl radicals generated during prostaglandin endoperoxide synthase turnover. Detection of the radical derivative of tyrosine 385
25. Characterization of the tyrosyl radicals in ovine prostaglandin H synthase-1 by isotope replacement and site-directed mutagenesis
26. An examination of the source of the tyrosyl radical in ovine prostaglandin endoperoxide synthase-1
27. Evidence for a peroxide-initiated free radical mechanism of prostaglandin biosynthesis