Construction of an Escherichia coli knockout strain for functional analysis of tRNAAsp

Journal of Molecular Biology

Volumn 310, Issue 3, 2001, Pages 537-542

  McClain, William H ^a   Gabriel, Kay ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Aminoacylation; Multiple knockout; Plasmid switch; tRNA; Two plasmid system

Indexed keywords

ASPARTIC ACID; TRANSFER RNA;

AMINOACYLATION; ARTICLE; CHEMICAL STRUCTURE; ESCHERICHIA COLI; KNOCKOUT GENE; NONHUMAN; NORTHERN BLOTTING; PLASMID; PRIORITY JOURNAL; RNA ANALYSIS; STRUCTURE ACTIVITY RELATION;

ESCHERICHIA COLI;

EID: 0035854485     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4785     Document Type: Article

References (13)

1. Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12
2. The complete genome sequence of Escherichia coli K-12
3. Gene replacement and retrieval with recombinant M13mp bacteriophages
4. A set of plasmids constitutively producing different RNA levels in Escherichia coli
5. Expression of synthetic suppressor tRNA genes under the control of a synthetic promoter
6. Plasmid systems to study RNA function in Escherichia coli
7. BAD promoter
8. Curing a plasmid from E. coli using high-voltage electroporation
9. Changing the identity of a tRNA by introducing a G-U wobble pair near the 3′ acceptor end
10. Translational efficiency of transfer RNA's: Uses of an extended anticodon
11. Ala by alanyl-tRNA synthetase
12. Section 25, formulas and recipes, handbook
13. Appendix B: Preparation of reagents and buffers used in molecular cloning