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Before surgery, rats were trained to self-administer food. A monopolar electrode was implanted in the MFB (AP + 5.4, ML ± 1.6, DV +1.6), VSUB (AP + 2.7, ML ± 5.2, DV + 2.4), or CBL (AP - 2.6, ML ± 2.5, DV + 5.0); coordinates relative to interaural line (65). Electrical stimulations were constant-current injections administered by pulse generator and stimulus isolation unit. Currents were square, cathodal, pulse width 0.4 ms, intensity 400 μA 200 pulses per stimulation. Stimulation patterns were "sham": The rat was connected to the stimulation apparatus, but no actual electrical stimulation was delivered; 2-Hz repetitive; "theta burst": 40 trains of five pulses, frequency of trains 5 Hz, frequency of pulses within train 100 Hz. Means and variances of the number of lever presses of different treatment groups were compared with one-way analysis of variance and post-hoc Tukey tests.
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We thank L. Brown, P. K. Stanton, and M. Makman for helpful suggestions; D. Colon for administrative assistance; and W. Paredes for technical support. Supported by the New York State Office of Mental Health, the New York State Office of Alcoholism and Substance Abuse Services, and the Julia Sullivan Medical Research Fund.
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